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8hsw

CRYSTAL STRUCTURE OF BAT MHC CLASS I MYLU-B-67 FOR 2.1 ANGSTROM

Method: X-RAY DIFFRACTION Dmax: 74.1 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ig-like domain-containing protein

Myotis lucifugus

UniProt G1PNR4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain A； UniProt 22–301 Not recorded Beta-2-microglobulin × 1 （L5K3Y9） THR-PRO-GLN-SER-ALA-PRO-HIS-GLY-VAL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 8；300 K；1.2 M Potassium sodium tartrate tetrahydrate 0.1 M Tris Resolution 2.03 Å R-free 0.262

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8HSW Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
8HSM CRYSTAL STRUCTURE OF BAT MHC CLASS I MYLU-B-67 Deposited 2022-12-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 22–301（280 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.3；300 K；0.2M Sodium fluoride,20%w/v Polyethylene glycol 3350,
Resolution 2.20 Å R-free 0.258
8HSO CRYSTAL STRUCTURE OF A MUTANT MYLU-B-67 FOR 2.2 ANGSTROM, 52M 53Q 54Q 55P 56W DELETED Deposited 2022-12-20 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 22–301（280 aa）
Mutation：52M,53Q,54Q,55P,56W DELETED No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；300 K；1.0 M Ammonium sulfate 0.1 M Sodium acetate pH 5.0
Resolution 2.10 Å R-free 0.272
8HT1 CRYSTAL STRUCTURE OF A MUTANT MYLU-B-67 FOR 2.4 ANGSTROM, 52M 53Q 54Q 55P 56W DELETED Deposited 2022-12-20 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 22–301（280 aa）
Mutation：52M,53Q,54Q,55P,56W DELETED No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；300 K；0.1 M Calcium acetate hydrate 0.1 M Sodium cacodylate 12 % w/v PEG 8000
Resolution 2.40 Å R-free 0.277
8HT9 CRYSTAL STRUCTURE OF BAT MHC CLASS I MYLU-B-67 FOR 2.2 ANGSTROM Deposited 2022-12-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 22–301（280 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；300 K；0.2 M Ammonium sulfate 0.1 M Tris 12 % w/v PEG 8000
Resolution 2.20 Å R-free 0.253

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1PNR4_MYOLU
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–280； UniProt 22–301

Beta-2-microglobulin

Pteropus alecto

UniProt L5K3Y9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count Chain B； UniProt 187–278 Not recorded Ig-like domain-containing protein × 1 （G1PNR4） THR-PRO-GLN-SER-ALA-PRO-HIS-GLY-VAL × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 8；300 K；1.2 M Potassium sodium tartrate tetrahydrate 0.1 M Tris Resolution 2.03 Å R-free 0.262

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8HSW Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6J2D Crystal structure of bat (Pteropus Alecto) MHC class I Ptal-N*01:01 in complex with Hendra virus-derived peptide HeV1 Deposited 2019-01-01 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 187–278（92 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.2 M NaCl, 0.1 M Bis-Tris (pH 5.5), and 25% (w/v) polyethylene glycol 3,350 at a concentration of 7.5 mg/mL.
Resolution 2.31 Å R-free 0.282
6J2F Crystal structure of bat (Pteropus Alecto) MHC class I Ptal-N*01:01 in complex with Hendra virus-derived peptide HeV2 Deposited 2019-01-01 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 187–278（92 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.075 M HEPES (pH 7.5), 15% (w/v) polyethylene glycol 10,000, and 25% (v/v) glycerol at a protein concentration of 10 mg/mL. Single crystals of Ptal-N*01:01/ EBOV-NP1 were grown in 0.1 M succinic acid pH 7.0, 15% w/v polyethylene glycol 3,350.
Resolution 1.90 Å R-free 0.231
6J2F Crystal structure of bat (Pteropus Alecto) MHC class I Ptal-N*01:01 in complex with Hendra virus-derived peptide HeV2 Deposited 2019-01-01 Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 187–278（92 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.075 M HEPES (pH 7.5), 15% (w/v) polyethylene glycol 10,000, and 25% (v/v) glycerol at a protein concentration of 10 mg/mL. Single crystals of Ptal-N*01:01/ EBOV-NP1 were grown in 0.1 M succinic acid pH 7.0, 15% w/v polyethylene glycol 3,350.
Resolution 1.90 Å R-free 0.231
8HSM CRYSTAL STRUCTURE OF BAT MHC CLASS I MYLU-B-67 Deposited 2022-12-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 187–278（92 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.3；300 K；0.2M Sodium fluoride,20%w/v Polyethylene glycol 3350,
Resolution 2.20 Å R-free 0.258
8HSO CRYSTAL STRUCTURE OF A MUTANT MYLU-B-67 FOR 2.2 ANGSTROM, 52M 53Q 54Q 55P 56W DELETED Deposited 2022-12-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 187–278（92 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；300 K；1.0 M Ammonium sulfate 0.1 M Sodium acetate pH 5.0
Resolution 2.10 Å R-free 0.272
8HT1 CRYSTAL STRUCTURE OF A MUTANT MYLU-B-67 FOR 2.4 ANGSTROM, 52M 53Q 54Q 55P 56W DELETED Deposited 2022-12-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 187–278（92 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；300 K；0.1 M Calcium acetate hydrate 0.1 M Sodium cacodylate 12 % w/v PEG 8000
Resolution 2.40 Å R-free 0.277
8HT9 CRYSTAL STRUCTURE OF BAT MHC CLASS I MYLU-B-67 FOR 2.2 ANGSTROM Deposited 2022-12-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 187–278（92 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；300 K；0.2 M Ammonium sulfate 0.1 M Tris 12 % w/v PEG 8000
Resolution 2.20 Å R-free 0.253

6 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name L5K3Y9_PTEAL
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 4–95； UniProt 187–278

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8hsw

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8hsw
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8hsw
Deposition date deposition_date2022-12-20
Structure title titleCRYSTAL STRUCTURE OF BAT MHC CLASS I MYLU-B-67 FOR 2.1 ANGSTROM
Keywords keywordsMHC, IMMUNOLIGY, IMMUNE SYSTEM TRANSFERASE COMPLEX., IMMUNE SYSTEM; IMMUNE SYSTEM
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier24.18 Å
Radius of gyration Rg (electron density) rg_electron23.06 Å
Forward intensity I(0) i035213300.00
Molecular weight molecular_weight44249.0 kDa
Excluded volume excluded_volume54688 ų
Envelope volume envelope_volume67164 ų
Hydration-shell volume shell_volume24465 ų
Envelope diameter envelope_diameter75.7 Å
Shell Rg shell_rg29.80 Å
Envelope Rg envelope_rg23.13 Å
Shape Rg shape_rg23.03 Å
Total Rg total_rg23.95 Å
Total atoms total_atoms3129
Residues n_residues386
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax74.1 Å
Rg (real space) rg_real24.11 Å
Rg uncertainty (real space) rg_real_error0.45 Å
I(0) (real space) i0_real3.5210e+07
I(0) uncertainty (real space) i0_real_error5.3440e+05
Rg (reciprocal space) rg_reciprocal24.13 Å
I(0) (reciprocal space) i0_reciprocal35210000.0000
Solution quality estimate total_estimate0.9142
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary30.1 Å
Skewness Skewness skewness0.232
Kurtosis Kurtosis kurtosis-0.493
Angular range angular_range— – 0.3300 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha7715000.0000
Real-space data points n_real_points65
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.965; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.987
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (4)

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8. Citations (1)

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9. Files and Curves (10)