8oh4

Subtomogram averaging structure of cofilactin filament inside microtubule lumen of Drosophila S2 cell protrusion.

Method: ELECTRON MICROSCOPY D_max: 219.7 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8oh4
沉积日期 deposition_date	2023-03-20
结构标题 title	Subtomogram averaging structure of cofilactin filament inside microtubule lumen of Drosophila S2 cell protrusion.
关键词 keywords	Cytoskeleton, Filament, Actin, Cofilactin, CONTRACTILE PROTEIN; CONTRACTILE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	65.21 Å
回转半径 Rg (电子) rg_electron	66.79 Å
零角强度 I(0) i0	1688590000.00
分子量 molecular_weight	211710.0 kDa
排除体积 excluded_volume	208130 Å³
包络体积 envelope_volume	597180 Å³
水化壳体积 shell_volume	84095 Å³
包络直径 envelope_diameter	251.6 Å
壳层 Rg shell_rg	55.39 Å
包络 Rg envelope_rg	65.19 Å
形状 Rg shape_rg	66.80 Å
总 Rg total_rg	66.53 Å
总原子数 total_atoms	15104
残基数 n_residues	3776
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	219.7 Å
Rg (实空间) rg_real	66.23 Å
Rg 误差 (实空间) rg_real_error	2.04 Å
I(0) (实空间) i0_real	1.6870e+09
I(0) 误差 (实空间) i0_real_error	3.5080e+07
Rg (倒空间) rg_reciprocal	64.09 Å
I(0) (倒空间) i0_reciprocal	1682000000.0000
解质量估计 total_estimate	0.7878
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	54.9 Å
偏度 Skewness skewness	0.647
峰度 Kurtosis kurtosis	-0.221
角度范围 angular_range	— – 0.1200 Å⁻¹
当前正则化参数 α current_alpha	0.0075
最高正则化参数 α highest_alpha	126600000.0000
实空间数据点数 n_real_points	25
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.724; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.886; Smooth: 0.180

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Actin-5C
分子量 formula_weight	41161.0 kDa
EC 编号 ec_number	3.6.4.-
突变 mutation	6 N-terminal residues (MCDEEV) were removed. Residues 42-50 (QGVMVGMGC) were removed
来源方法 src_method	nat
拷贝数 entity_count	8

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H
原子数 atom_count	15104

AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Entity 2 polymer

描述 description	Cofilin/actin-depolymerizing factor homolog
分子量 formula_weight	17180.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	6

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	I,J,K,L,M,N

MASGVTVSDVCKTTYEEIKKDKKHRYVIFYIRDEKQIDVETVADRNAEYDQFLEDIQKCGPGECRYGLFDFEYMHQCQGTSESSKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASREAVEEKLRATDRQ

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7. 引用文献 (1)

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分辨率 resolution	16.50 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	3801

fit	/app/data/saxs_computation/oh/8oh4/8oh4.fit
pddf	/app/data/pr_computation/oh/8oh4/8oh4.pr.out
profile	/app/data/saxs_computation/oh/8oh4/8oh4.dat

Subtomogram averaging structure of cofilactin filament inside microtubule lumen of Drosophila S2 cell protrusion.

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)