8ruy

Structure of IFTA and IFTB in Retrograde Intraflagellar transport trains

Method: ELECTRON MICROSCOPY D_max: 433.8 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8ruy
沉积日期 deposition_date	2024-01-31
结构标题 title	Structure of IFTA and IFTB in Retrograde Intraflagellar transport trains
关键词 keywords	Cilia, IFT, train, retrograde, PROTEIN TRANSPORT; PROTEIN TRANSPORT
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	154.00 Å
零角强度 I(0) i0	54477200000.00
分子量 molecular_weight	1983600.0 kDa
排除体积 excluded_volume	2476800 Å³
包络体积 envelope_volume	5519000 Å³
水化壳体积 shell_volume	328900 Å³
包络直径 envelope_diameter	649.7 Å
壳层 Rg shell_rg	109.40 Å
包络 Rg envelope_rg	149.60 Å
形状 Rg shape_rg	154.00 Å
总 Rg total_rg	153.80 Å
总原子数 total_atoms	139391
残基数 n_residues	17586
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	433.8 Å
Rg (实空间) rg_real	146.20 Å
Rg 误差 (实空间) rg_real_error	2.42 Å
I(0) (实空间) i0_real	5.2350e+10
I(0) 误差 (实空间) i0_real_error	1.3440e+09
Rg (倒空间) rg_reciprocal	124.80 Å
I(0) (倒空间) i0_reciprocal	49120000000.0000
解质量估计 total_estimate	0.9036
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	173.5 Å
偏度 Skewness skewness	0.357
峰度 Kurtosis kurtosis	-0.642
角度范围 angular_range	— – 0.0500 Å⁻¹
当前正则化参数 α current_alpha	1.7230
最高正则化参数 α highest_alpha	1406000000.0000
实空间数据点数 n_real_points	11
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.071; Oscil: 0.998; Stabil: 0.957; Sysdev: 1.000; Positv: 1.000; Valcen: 0.894; Smooth: 0.000

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (20)

Entity 1 polymer

描述 description	Intraflagellar transport protein 144
分子量 formula_weight	150972.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	139391

MKKLFGVGPDLLGDGKVLFEWSPKGNFLAAAGSKRKVNIFDRNGRLYDEVHFPPAEYPNPDGRACAAAQMQWDPAGEQLAILPAGNTHVFIWLAGNKEVQKIESEFKTQEFSCMAWSRNGMYLGVATVKGNVMMYNARERKKTPLVGKHTKKIVAAAWNKDNIMALAGQDKTVTLTDGVTGDTIKTFHLKDVPMDLCVSDKKEDGYSRREENTYSLNINRKTLYIMQCTAEGDRPLELAFLDTYGPIMKHSWFGDGYILLGYKNGYVAVVSSHSREISEEVHSGKYLDTLTDVTYCASLGRVAMAGANCVRVLDANADYNEIKGDAVDLDANQAIEKVGWTKDGQVLTVGTHNGYMHSFLASLPMVYDFHGTRVLYLTSLLEMTLLDVSRRQTVARIELENEPAFCGLGPSHAAVGMNNQAAFYSLGEKVGKVVQRREYLGTITAIKLNETQAAVLTGGHVVVHPISVEAGHAPDELDVVIPGPGQPANITCVALTPTFVITGSRTGTLSYYLSPDVTPVNEFRHDDGGIVRLFPQATGARLVFEDDKGALHLFNPVNDHVVAVPYTGRAETVMWDTSDTNVMVIGDGTALHSFLYVPVSLTGPQVQDLGKQAVPATHTPLTVCNGVVGCRLKSGAMDNVTLESHKMLQPGDAVARAAPAKRFAAALKLYKLRDAVECAKQLRQVESWRTLALAALDVLDIDTAINAYREIGDASMVLSLERVRQHEDRNLLSAHIMVLLEKDYGQAQELFLRSSVPRAALEMRMDLKHWTDALKLAEQLDPDAIATICKEHGAMLEMTGEYSNAKSHYQQALDALAVSVGPAQPDLEAACKAGIARTTLQLGDLRQGRQLAMQLNSQTLFKECALILEGLQQLTEAAEMYERAGQFERAASIYIQTKNFAAAAPLMARISSSKLQLQFAKAKEAEGRWQEAAAAYEAAGDMDAVVRLCLERLSQPQRAYAIVRKTQSVEAANQLSRFCLQSQDFGGAVEFLLMAGQMDQAFDIAMGHNEMDTFARIVAASAKPVDYQRIAQYYESRGEYDKAADMWSKCDQAPRAVQLYLKVGTNPALEKAVQVVEQTRSHQLGVLVLDYVNEEKDGTTRDEFRFKLNIAMGQFAEAARDALEMARFEQEEGNYRVAHDKLFGTVKQLEALNTKPPGELLRALMLLHSYTLVKSLIAINDHTTAARMLVRVARNISKFPKHIVPILTSTVIECHRANLKKTAFEYASMLMRPEYRDQVAVKYKKKIELMVRKPEKDPEELEEPLADCPFCNMPGPETELQCISCQNILPFDLATGKRMVLSDWAECPGCKFPASASQFIRIISAEGRCPMCNDPVDLAHVRKVQDPLTKIKQQQAQQTNASGGGAS

Entity 2 polymer

描述 description	Intraflagellar transport particle protein 140
分子量 formula_weight	157426.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

MEIYYTTKISGRAGVIAGWSKAPGYPSLLAVALSPNAVGIYNEEGRPLDPNNEANIKNVRGTECAKIAWHPLLPLLAIGWKDGAISFWNAEERKLEEDSKIHRNTISSMTWTASGDRLITGDENGRISMWKTDRLMRPIHVVAYDEPGAVIRHTVVGLPEEMPDTNSQVVVAFYVADAQERAVVKWCNDQGYSGVVVEMSEGVHTLVHYPERDQLLVVGSSCTLNVLTRDEQLGTWVTASKMKFATGTGEAATGLQVAWAGNHTLASASEKDNVVRMYNFDTEDNYVLNLEQDSGLVSRVVCLAYDDRYNLLAVGTTDGRVMMYKFNQPNLNLEPVLDFAKCWEMQPAFFVGNRALAMEWGPFPRLMVVACNDAINVCRKTMLSYKFRDGVAIMQVAVDRVVLENLEVEPQRPPGRLQLQDMQLLGLDLSKGLLLVWDGERAEVYKVTETNDIMPASQFETTSRCMAINNDSVYRAAENKVEVVNMAGTVKQTLMFDDNHGSPTSMDVARDYLAVVTSANIVRILKVAGREAKPHAGPAPLCPPELKDWKVMAMRANGNGNLVAALVVGPEPARAVRLVVWCGETNTHHVHDFTPEGRQPVQLMFDTVEPKLLVVQCTPVFTEANVDERPHGDAAHVVDCAIIFVDPDKGILLQEYQPIHTGGATACIGSCAPHLLTNKKSMVQPAPGSGAFQPFTSNVSKAIMTSFQGMQDSDDKTRRALLDFSFNLATGNMDEAFRSVKAIKNPAVWENMAHMCIRNKRLDVAEHCLSNMEHARGARALREAKSIEEADARVATVAVHLGMIEDAKKLYIACERYDLLNQLYRACGQWDKALEVAEKNDRIHLKSTHYAYGQFMERQGDMEGARKHYEAAGCGLVEVPRMLFEAEKFTELQNYIQANDSRELILWWGKYLESLGEYAKALDCYRKAGDSLSMVRIHCFQRDWKAAEDEVTNSADNAASFHLARQYEASGRIPEAIRYYTLAKRYSHGVRLAKTHELDSDLMNLALKSTPAVMIDTADYLFAKGQHEKAATLYMKGGKLSKAVEMCFQAQLFDVLQHITDDMTPEKSDPNLYNKCAEFFMGFGHNDKAVKMLIAAQQYGRALELCVEHDVSITEEMADSMTPDKNAAVSADERNNVICRIAKVAKRQGNFQLAAKKYTQAGDKVKAMKALLRGGDAEKIIFFAGVSRQKDIYLMAANYLQTLNWHSDPELMKHIISFYTKAAAWESLASFYEACAQIEVDEYRDYEKALQAMREAAKYVAKSKNDDRDARVGVINDRIAVAEQFVAARQLIGSNPQEALRVCDELLRAIPPNSQDLEAGIRIGDVYALMVEYWYEARNPNEAYKAIEAMRRRGIILSPYLDTRMVEDIYRSLGVALDMAEERRGPANLGLRESDAGAFVEEEVADEDD

Entity 3 polymer

描述 description	Osm-6-like protein
分子量 formula_weight	50453.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	H,h

MEEPGAEEVRILFSTAKGESHTHKAGFKQLFRRLRSTYRPDKVDKDDFTLDTLRSAHILVLGGPKEKFTAPEVDMLKKFVKNGGSILILMSEGGEEKAGTNINYFLEQFGMSVNNDAVVRTTHYKYLHPKEVLISDGILNRAVITGAGKSLNSNDDDEFRVSRGPQAFDGTGLEYVFPFGATLSVQKPAVPVLSSGKIAYPMNRPVGAVWAQPGYGRIAVLGSCAMFDDKWLDKEENSKIMDFFFKFLKPHSKIQLNDIDAEEPDVSDLKLLPDTASLADKLKGCLQEIDDVPRDWTSLFDDSLFKFDTGLIPEAVSLYEKLGVKKGQLNLIPPSFETPLPPLQPAVFPPTIREPPPPALELFDLDESFASETNRLASLTNKCHGEEDLEYYIMEAGHILGLKLQENANAKHVLSEVFRRIAQYKMGSLGLGQTLDSMGQTLPAANQFGDQFEL

Entity 4 polymer

描述 description	Intraflagellar transport protein 80
分子量 formula_weight	85782.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	J,j

MRLKVKQSSANVHSELTAAVGWNVWNELFTCSDDQTIHKWNMLGEPEQKVSTLDAYFTDMHWYPVSSKKTQAGGTDVFAVACTDGSVKILSRTGRVEKSIEGHKGACISLRWSYDGTALATAGEDGSVKIWSRNGMLRSTLAQADSPVYSIVWAYDCDQLCYCTGSNVVIKSLSSNAKQNAWKAHDGVVLKVDWSPINHLIITGGEDCKYKVWDSFGRLLFQSGLFDYPVTSVAWAPSGELFAVGGFNTLQLCDRMGWAYSKIHLNDTGSIMTLSWTADSTQLAGGGGSGGVVFGQVVDLALEDGKMQVTVVDDMRIVVNDILNENADELPEFRDRVIKVSLGYGYLIVATATQCHVYNTTNLGTPHIFDLKDTVTLLLQAERHFLLLDNSAGIQIYTYEGRQICNPRFQGLRTELLNAQMITLSNDTIAVLDQQASGTTVRFFDTAQGRPVGEPWQHTLEVKEIALSQAGTINDRQLIVIDRNRDLYLLPVMKRHVAKLAAMCDSARWHDSTAMLSAMVDQRLCVWYYPSEVYVDKDLLAKTRYTKSDSDFGKSAQIQLFAGNRCLVRRSDGVLVSAATSPYPAVLYDMIRKQQWDKATRLCRFIKDPTMWATLAAMAMAAKELNTAEVAFAAIDEVDKTHFVRKVKQIPTEEGRNAELAVYRRKPEEGESILLQAGLVFRAIKLNIKLFNWERALDLATQHKQHQDTVLWYRQQFLKNAKLAESITRFMQMNESVVVDQAAVKKKIEEERIKESQRPGAKRYV

Entity 5 polymer

描述 description	IFT54
分子量 formula_weight	54437.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	K,k

MCDNWQATIDTLQGASPVFDKPKLSQKLLEKPPFRFLHDVVTAVQQATGFAPGLYQGDELDGKAIQEKDAKVAYLKKIIEVVSMVLGEQCPARPNKIVAGLEPENTNIFLQMLGRACQKGNGAKAVQKVLGGGGAEPAPAKEEAPPPEKKPEKKEKKEEKPAEKSRAEASPARKKAAEPEAEKKSSSKSSSRTKEEPPAKAPAKKKEEPAPEKPSKSKAAPAAEEAPPPPPPPAAEPPARSASPGGEDPLNKSGSAAPKFQRPTSARKAPPRVPQPQQPTMLAGTGIRPGTATRRPNEPKPTDSKVTKPVAVFTDNAKDNSDDEVEVVHEQTPVLSGGANMTGEQGVLVKDILAAEKGLKKAGVDATADNADTSDQGSTGIILKRLGGKAAGAGAAAAGPRAHDPSSVRELVQKLCHSSTPLAKSMDYLQEDIENMRKEYKFWLTEKRMYQDELARELRLQGEAANVDAQLADLDGQIKQARDRIIGMKGQILRNDETLGKLLAMATAGR

Entity 6 polymer

描述 description	Intraflagellar transport particle protein IFT20
分子量 formula_weight	15658.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	L,l

MDAVDRGVYFDEDFHVRILDVDKYNASKSLQDNTNVFINNIQNMQGLVDKYVSAIDQQVERLEAEKLKAIGLRNRVAALSEERKRKQKEQERMLAEKQEELERLQMEEQSLIKVKGEQELMIQKLSDSSSGAAYV

Entity 7 polymer

描述 description	Clusterin-associated protein 1
分子量 formula_weight	49733.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	N,n

MSFRELRSFTEVMKALGYPRLISMENFRVPNFELVADCLYWLVHRYYPGVEIADDISTESDRVKFLQSVAQVMLTKARMKLNIKRLYAADGNAVKELLKLASLLYKATSKAGDMDDDAAEAIDVANSLKGFNPKEIKGLAAEIIKAGAGLYDALGQEPELREHRARAVAGHVDTDFVERSIREAIAQVEDNIRSLENQMEDLERNEKTLDNKIEKKKQELERQEKRLSTLQSVRPAYMDEYERLQGELNGLYQSYLDRFRNLEFLENELEAYYQAEQEKMEAQERRLKKMQKRLKEEELRILRGEQEVDEANMNFDDDDMDLSEDGEHEEAEEGEDEDYGAGGQKATMRRDQFGGQGGGGGGGRQQGGGRNVMGNLDGDFDDEASIDEDDNTDGGEVSRQGSQAGGMGGGGGYGGGGGYMNGGGMDDDENNLIQDDDEDDNDF

Entity 8 polymer

描述 description	Intraflagellar transport protein 74
分子量 formula_weight	71648.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	O

MDRPSSRGALALGAGGLGKAPTGGAVQQPDRPMTGQRGAAPAGPMRAPAGASIIGAGPPGTAMRGGPGPAGGPPGTAYKRMGTASQRPGTGQQAAAAAAAARAGQQLQVENRPITNHGVSGMKTAAAGVGRQVLDKNYFMNELRQKRMEIAQVTSNMKSDLEALERKQAQYNSMDKRASDLSKEVKILQEALADYNTVLDKVGSQAPVYVIQQEFAALKDRNEQQRKRVDEVLTERLNLESKAKQAESKMSEIQASMDQRLNSMPPSQRNEYTTLVAEQQQLQADSKRFEEVLDELDKALQASEGELARNPFKQRSLQLQEQIRALTEKKYELTEEERQSKRSPEELRADLMAKIKRDNTEVEQMTQQIRELQDQIKKMEERVKSLGGATSGAAAAEEKANREKFEELLAKERDLNNFMDGFPSRKAAKMQEKQQKEDGIVGVLEKMVKMQGIIGSNLPSQKKYKEMQDELEYKKMQLENTQTTQERLKEELTMRRTELEKIDTLEDKIKLELTQLAERQEAMEKEMGEFGSVEDIQRKANAARERMEGLRSVLLKRKDLLRSIVAERGLKFQAKRAQLQDHNLQVQLEKMEAKLKNLSAGVFEMDEFIKAKESETNYRQLASNIAALVDDLNVHVKKAVV

Entity 9 polymer

描述 description	Intraflagellar transport protein 81
分子量 formula_weight	77186.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	P

MGDVSYIVDSLGLPPFSYQMSLLSFTEKGPQELLQLLSDVFSTISPKHQKVDVAKEVPDQTADRLIGFLKIIKYRPNVQDPLLFRQLVAAGDRETLYQILRWVVPQAQLLEKRAFVGYYLSFPDMPEEFNFDPDIMELKEEIKAMQQEFIELHKSSDAIKTLSKDTQALKNKIKSLEEEKERLGEKVERAKGAVDKLPDRSSYMEVCTNLRKQQDEEVNLSTAIQTQRALQEKAEASYHRAAARLRELQTSYQEGSAGKLLETLNEDVKNLRAQVNERYPKEVEKRQKRQAALSEALASGISTEMDLQRLQHQATALHNQITEIQERKVAQDKARQGDKAYLQLRQAQQMATVSARKKEELGAKQERLQEKKTALTAQLDKLNAEGGGSGAVFSEEEWRTKYESMKSKLPIYKKMKKELGDLEAEVFVLAHTEELLASQEGGLLEKVKRLEKQQGISGFTETAQHLEKVSEAKSQMDEEKGMTLIEISRTVEEINNAINQRKQQLAPQIKKLRSVRQDFAEFEAKYLEKKTAYDNVVATFEARTSALEGEVSGLKAEVSENETKYHMLHCQLHITDQNIKKVTSGPAAERLRDKYEAKVKEAEDSTKALRDRQREIKDTHSTGLSQIDIMNDMLRLLQLKLNLARGIAVDMSQYGGGGGAAGGANGGMAGQTYDTGSANVLQL

Entity 10 polymer

描述 description	Intraflagellar transport protein 27
分子量 formula_weight	22784.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Q

MVKKEVKPIDITATLRCKVAVVGEATVGKSALISMFTSKGSKFLKDYAMTSGVEVVVAPVTIPDTTVSVELFLLDTAGSDLYKEQISQYWNGVYYAILVFDVSSMESFESCKAWFELLKSARPDRERPLRAVLVANKTDLPPQRHQVRLDMAQDWATTNTLDFFDVSANPPGKDADAPFLSIATTFYRNYEDKVAAFQDACRNY

Entity 11 polymer

描述 description	Intraflagellar transport protein 25
分子量 formula_weight	20380.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	R

MKDYAREENGGLVVMASCSDERFPPENMLDGKDNTFWVTTGMFPQEFVLRLESCIRVSKITTLSLNVRKLAVEKCDQDKPDQFEKVFEVELANRGDRLQTEVHQVNIRAKYLKFILLQGHGEFATVNRVSVVGGDDDGGGYDEPGGGYGSMQRQPSMGYGGGGGSAATGFAADPGNAAAGGGGGFEDEF

Entity 12 polymer

描述 description	Intraflagellar transport protein 46
分子量 formula_weight	37976.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	S,s

MDDSMDYPDRDGDDLDQFQGTARSQVVQNQPHDEEVNLSESESFAGADEPPAAPRDASLIESHDMDEGPAAPARTLSPTGYEAGKHAPGGIANSDEAPPGAYNAQEYKHLNVGEDVRELFSYIGRYKPQTVELDTRIKPFIPDYIPAVGGIDEFIKVPRPDTKPDYLGLKVLDEPAAKQSDPTVLTLQLRQLSKEAPGAKADMVGRLEHTDENKAKKIQQWIASINDIHKAKPAATVNYSKRMPEIEALMQEWPPEVETFLKTMHMPSGDVELDIKTYARLVCTLLDIPVYDDPVESLHVLFTLYLEFKNNPIFRQHMEMENKLDGMSGGGGGMMGGGADVLGL

Entity 13 polymer

描述 description	Intraflagellar transport protein 22
分子量 formula_weight	21363.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	T

MAEHGLKIAVVGPQRTGKTLLCRALAEQPILLGEMSYQPTAAVRIQEISRVLGIDRVKVQFWDVSGSVQYQSYWPVLAKEVDGLLMVIDPNRPEQERDLETFYRNFAEPNNLYTRQCMVMAIQVQKEGGGLGGWQGLQGGLKKLSQSYVAINPANPAAGVQEAYSHLDVLFQGALQSKKEALESSYMNQEDQ

Entity 14 polymer

描述 description	Intraflagellar transport protein 172
分子量 formula_weight	197851.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	i,I

MQLRYFKSILPPADQYQKITSLTWAPNNSRLAAVSTDKVVYLFDENGEKRDKFKTKAAEANNPNTYIIRAMAFSPDSTKLAIAQSDNIVFIYRLVDPDTGAEKKSICNKFPQACAVTSLVWPKDRPNEVVFGLADGKVRLGMLKNNKSYTCYAHPENSYVVALASSLNGQNVISGHMDGAIWKFNFPAEEGGTPTSSQLVVHSCVPYSLGWGSCIAAAGNDNRVVFYDLNGREIRSFDYSNNDEVREFTTCAFNPSGDTVVFGTYNRFYMYTFNIQRNDWEEAGHKQIDNFYAVSAASWKPDGSKMTVGSMTGAVDMYDACVKRHMYKGKFEFTYVSKSAVIVKTLKTGMRIVLKSVYGYEIEKINIYHDRYLIARTTYTLLMGDLDTCKLSEIPWDSDGSEKFHFENERVCMVHYAGELHIVEYGRNDVLGTCRTEHMNPYLISAVVQEARGIASESKKLAYLIDLQTVRIQDLMAPVGSTLATVNHDTKVDWLELNQRGTHLLFRDKKRHLHLFSLSGQERTTLLNYCQYVQWVPGSDVIVAQSRNNLCVWYSVNKPDNVTMFPIKGEVVDIERHNHRTEVIVDEGINTVSYALDEALIYFGAALEDQDYERAVQTLEPLELTPETEAQWMQLAEQALATNQLVIAERCYAALGDIAKSRFLHKVVKKAQQAAKEFGGDGTDAWSVRAMMAQLNKQWPVSESLLLAQGKVDDAITLYQDNHRWEDAIRVADSTHHANAAALKQQYLTWLLETGQEEQAGAVKEREGDYLAAIGLYLKGGLPGRAAQVVMSVHNVNWDPALLDSILASLAKAGLYERAGELYEHMSRSSEAMQSYRRGHAYRKAIDLARREFPAEVIIIEEEWGDWLVTQKQMDAAINHFIESGATLKAIKAAIDCRQFAKAAGIIEVLDPREAMPYFRRIAQHYETTGALEEAERYYIRADMARDAVEMYSRAGKWEAAQRVARGYLTESEMRAFYRAKAAEFEAAHKLKEAEKAYLAAGGDDVDKAIAMYKRNKMYDQMIRLVTQYRKEKVPEAHTLIAQQLEVEGNLREAEKHFVEAKDWKSAVQMYRQVNQWEDALRVAKVYGGVNASKQVAYAWALTLGGDDGAQLLKKMGLLDHAIEYAVESGAFAQAFEMTRAGAKHKLPEVHLKYAMFLEDEGRFAEAEAEFISAGKPKEACDMYMHNQDWDAAMRIAERYDPTMVSEILVSQARVAVERKQWLPAEGLFIKAKRPEAALKMYRDARMWNDALRVAEQYLPTKVAEVQMELLSGQGAGGGSGGASADAVINKARGFERNNDYARAIETYLSLTAQDTSNQDQLEHCWGQAAQLAINYQRHRMKDVVNTVSERLQEIGRHQAAGELHESIDDAQGAIRAYCAGRLWDKARTLAGTNPTFSRYIEDQYNNYLLQNQQADELASRGGQHAQQAIEMYVARDEWAKVHELAAQQGPEVASNYALKHAERRFKQGDYAQAAQVFAQHGITAQPQYFELYKSIAQGVLHASQGDRNPVAEKSLRDMMYRLVNVLRSGGGAGKYKVDTDAFQNYYLAAHYLTCAAAAKEQGLKDIAAMNLTSVLRYVGPTIPADRAFYEAGLAWYEAGRKNMAFVMLNRFLDLSDAMDEPDSSAAVIENADFSDTDIPYDFTIPERAYCTESQREDVRNLVLEISMDRSSDQSLALKACEHCGKPTYEANLTCHFCKKKYDPCVVTGYPIQSYDRVVFKNNGPELNAIRDMWNKWVEAFGTDPVTGMQAAPMY

Entity 15 polymer

描述 description	Intraflagellar transport protein 122 homolog
分子量 formula_weight	139382.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MRTVVAWQETPPEKDGVRNVCYDLCFKPDGSQVVAGIGNRVLVYDAADGDLLHALKGHKDSIYCVAYAQNGKRFASGGADKTVIIWTSKAEGILKYTHNDAIQCLSYNPVTQQLASATGSDVGLWSPEQKSVAKHKVASRICSISWTADGAYLALGCFDGVISIRDKGGSEKHRIETGPSPVWSICWNPVETNVLCAGCFDGVLKFYMMSGQQKSKDRELAFDPLCVSYFSSGEYITVSGTDKTVHLYTRDGTYLTKIADRDSWVWAVRPRPKHNFVAVGTEGGGIAMFQLIFSTVHGLYQDRYAYRDQMTDVIIQHLITEQKVRIKCKDYVKKIAVYKDKLAVQLQNKVVIYELANADDFDMHYQSATKIQQKLDCNLLVVTSHHVILCQEKKLQLYNFDGVKEREWVLDSVIRYIKVVGGPPRREGLLVGLKSGAILKIFVDNPFPIPLIKHTASVRCLDLSASRNKLAVVDENAKVLVYNLITKELVFEESNANSVAWNSEFEDMFCYSGNGMLSIKTGDFPLHQQKLQGFVVGFKGSKIFCLHYVSMQTIDVPQSASMYRYLERKDFESAYRVACLGVTEADWKQLALEALQALNLEVARKAFIRIRDVRFVELVNRTEAGRKAGTSEQLLLAEIMAFQGRYQEAARLFTQAGAVDRAMEMFSDLRQFDEAKKWAEEFAASGRGDQRSVQELINRQAEWSEEVKNYDAAAEMYIKAKKYDRAIAILAKHQWWDKLIGVVRQLDKTDARCLGMCAGHFRRAPHFAYAKETLLKMDDTKGLITLYVEAEKWDDAFLLLHAHPECRQDVYLPYAKWLSNQDRFDEARLAYQEGGFPSLATRILEQLCANAVVETRYADAAFYYYQLAMEALKSIKNPPSNMAPSDRSALERFTELYDRAEVYYAYEVVHKSVHSPFRTTHPDTLFNASRFLLMRLLPPREVPLGVSVVNVVYVLAKQAVEAGAFKLARFAYNKLQTLVLPAAWQAEVDLASVVIRSKPFSDKEDLLPVCWRCSTTNPLLNTQGDYCINCGAPFIRSFVTFEHLPVVEFELEPGVDDEEAGRLLGEDAGMEAARRERKAERQAKAAEVGGNMLRLDQNEIDRMDDAFAAQMMVPNTTIRVDRAMLRRLKTAEVMVRTWPNPVIPKQYFRVMDQEVPLCCGPCGHFFEQDEFEMAALERGTAPFSRTTVRGEGLAPGEDAEDEGAGGNKLGGPLGSARGPIGGASKARMSVPFQQGRPLV

Entity 16 polymer

描述 description	Intraflagellar transport protein 121
分子量 formula_weight	136267.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	D

MFIYLSKKIAIPNNVKLRCISWNTDQGWIACGGESGLLKVLRLDGTTSKDVKGKKEGGAPGGNLSLNQTLEGHNGAVICVCWNNAFQKLTTSDEFGLIIVWMLHKGMWFEEMINNRNKSVVKDMKWTSNGEKICIVYEDGAVIVGSVDGNRLWGKELGMDLALVEWSPDGRLLLFATPGGECHVYDGNGNGVAKVPLYCNEGYAGASKIIGVEWYDGVEGYAEPNCPVLAICLDNGRMQLMRYESDDNAVCIDTGIKPVKVKWNMNGTILAVAGFQMSSAVADGRELWMVQFYNHNGEHLRTLRVPGGGISGISWEGNGLRLALSVDSYVYFANVRPDYKWGYFCNTLVYAFNRPDRSEHCVMFWDTKNNDKYPKYVRKLVAIQAFGDFCVLATKGENPGEHILILCNAIGSPVDSKYIEVEPKYLAITNYHVIAANDEVVYVWQFRTSFSKVLSTDINAVKRKDVREKMFSVDDSNPALSDRSPESFRNMTTQTTDPIACVTACDSVLMIGRASGVVNRYSLPHLTLDAQHVLRCRPQMLALNCNLSKMSIIDINGVLTFFDLTAKAPGGGANTMGEHLSFERKDAWDMRWADDNPELFAMMEKTRMYIFRGLDPEEPVTSSAYLCSFHDLEITAAFLDDIMQQPDQPDLEFMVMYETRSLRDTRELLKSASVDDAYAFVDSNSHPRLWRNLAEHALEQLDFTTADKAFVRCADYQGIQFVKHLGKLDDKAKQRAEVAVYFKRFDEAEQLYMRMDRPDLAIDMRMRLGDWFKVERLIRESSGDDAQLLNAHNKIGQYYSDRHKWGKAAQYYAQAKNSEMLVECFYALEDFVALGRLMDALPDGSPLLANIGEKFQSVGLCNEGVTAFLKAGDTKRAIDCCVLLNQWDQAVQLAQAHNFPQIEQLLAKYANHLLEKEKVMDAIELYRKANHSMEAARLLQDLAKKCADQKVHPLRVKKLYVLAALEIEKFKKRTLDMAGPDKTAATLLGTGGTATMAATAAQTLAGLMTLESAVSGEAGVDSAWRGAEAYHFWLLAHRQLYAGNVDLAMRTALHLREYEDLLDPVEIYSFLALAAFYNQFFGQCSKAFIKLESMPSIPNDKRESFADLAMSIFLKHPPADPRALRETREKKAPGGGAMAGNSLDALLEDLGGGREQVCVASGRIVRDGNVVRCKVCKHLSITHELHGAAVCPLCHAALPVSGGGAPGRGMSAKGGAMAQMYGAY

Entity 17 polymer

描述 description	Intraflagellar transport protein 139
分子量 formula_weight	152227.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	E

MADRVLALVHYYAREGYFRHVQTVCNEVLKKRPGDGVLTFWRAYGLLMEGNTADAMRDLSSIQGNSDLELAVAAAQLLGHESAKVPDHDAIIDLQAKLEIEERTASDQPCLHLASFYLYTKSKERARGLVERVLRNQPDMVPAQVLLGWIIISQQQDDEYDMLFDESELDDALSHFEQAVEHDHNDLQALLGKAKIMELKKQLGPCLDVLTEINVRFGWFVPALVEKTRMLMMLGDWEQVTETLQRVLAADQQNIMAQAWNCMISLTREGNNKQAAKQLQDLFSSMNRQEPKNAELFFRVARPFGRLACSDPTLLGITYLMADRAAQLRPEMAAYVVEAAAQKLMMDETTNATERFTQALQLDELNLEANAGALEAQIMAGELEEAAGQIMFLEDMFTNAAAAGGGKRKGRGTGDMDDDPDMADPSLGTSSDNPTLLYLKGLLAWKQGMPSEGLGLLERSIAALFSAAADFHGPSLELYAALNPARITAVVRLLLQSIGGEPRAPTEAPSPLISKVTRALDLLNKQAPALQESALLHARALYLNGNLDGALRKAGEILRMNPEESSAHLLICSVYVAQDKPELAVSALDQAVSSNFAIRETPLYHVVQAKVLVANNKLDDAKRVLESAMNLPGVRTALTVQQRARLGRKVVEPTLHERATVYLLLADVLARQSKIPDAPEAKKYIQDAIREFEGTSEEVRVTVADCELAIARGDVEGALKKLRRIPKESPHYVKARMAMADIYLRHRKDKAAYIKCYMDLVDHTPDYDSYCMLGEAFMQIQEPEKAVRAFESALEFSPKDVDLITRCARALVTSHDYQRAIDYYTKAIANARGGAQHALQLELGNLLVRLRQYPAATAAINKALERNRDGLPATENLQLDVEAWSMLAKVHKGKLEMEGYSQAQSRALELQKQLLLKLRGELPEAVALQRERTAAICFDLAEQSKRARQFDRAMELYMEALRHHDTHVPSMLAVAKLHLANGDTDACQAQCVTLLKHDPDNEEASIMLAELMFHKEHYDTAIYHFQQLLERSPNHYGALAQLILLLRRAGRLEDVPRYFALAEAGSPKAVMDPGYHYCKGIYNRYINNPREALKELNLARKDTRWGSQAILHMVEIYLNPDNDAVWEEKENADTPESRQAVATARSLLKQVRGADTTTQRYRVLESYAIMAGKDKNEIEASLNVLLDLANQDPNNVPVLLAMATGFMMLKQTPKARNQLKRVQKIQYKPDEAEEFERSWLLLADIHIQGGKYDLAQDLCQKCLKYNKSCAKAWEIMGQIMEREQAYKDAADHYENAWKHENQASAQVGFKLAFNYLKARRYVEAVDVCHKVIKAFPDYPKIRKEILEKARMGLKP

Entity 18 polymer

描述 description	IFT88
分子量 formula_weight	86418.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	F,f

MSYGGTEEDDLYGGYDEQSNPLAGSGGAAFKALGADGAPPGTAMMGPPGTAMKSFVPGTAMRGGTAMQQDPSLARPMTSNRGAGFTSAPNKKFDPLNRSMGSTLGSSGGGAMLVARKGDTSPEEQARGMEKTVHELLEKSAADAAKNDINSALENAMEAKKNERKLCRFREQNNMADQINLELMYAVDFNLAHMYHMNKNYSEALNLYTAIVRNKNFPQSGWLRVNMGNIHFEQKKYPSAIKMYRMALDQISATAKEVRFKIMRNIGLSFVRMGQYPDALQSFATVMDNVPDHQTGYNLVMCNYALSDREGMKNAFIKLLKVSPPSEMDDDDDDDPMGDDDMQVMTMDDGLKDEMRKRNTIITRLIVKAAQLISEKVDRANGFEGGFMWCCEQLRDAGYTKLANEVELAKATRFMGQKQFDKAVGVFKDFEKKEPRVKARAATNLAFLYFLEGETDQADKYSEMALKSDRYNARAYVNKGCVLVERGDLEGARSLFNEAAGIDPYCVEAIYNLGLVSQRLNELPYALAAFKKLHNMVPDNVEVIHQIATTYDMMGDFKNAVKWFELLTSLVSNDPGVLARLGAIHARFDDEAKALHYYQESHRVYPVNMDVISWLGAYHVKSEVYEKAMPFFDLASKIQPQEVKWALMVASCYRRTNNLPAALGKYKQIHTQHPDNVECLRYLVHLCSELGRRAEAAEYMTKLKKAEKAAVPEATTAAAPAAAAAGSGMGGMGGLDDDIGSSAVSAQNRGKKMLVKEHMGGGGGKDNDDWGNEQLGDDLLPM

Entity 19 polymer

描述 description	IFT70
分子量 formula_weight	74248.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	G,g

MAFFQQPARPIAEGQYTQTIYTLIKEQKFAEAIQHLQYQLQNVPESRAALSLLGYCYYYTGQYDMASQMYEQLVTLYPSNEDYKLYYAQSLYKGGMYPEASKAVVKVEGHQKAVTTLLVACSYEQDDLTGCRRQLDKCAPEDPDTMVNTGCIMFKEGKFEAARQKFNDAVQALGYQPELLYNIALCYYKTKQFGPALKHLAEIIEKAVREHPELSVGSNTDGMEVRSVGNSQTLKETALIEAFNLKAAIEYTMKNVEAAKEALTDMPPRAEEELDPVTLHNSALINMDSDPTGGFKKLNFLLQSPPFPPETFANLLLLYCKPSHGFYDLAADVLAENPQYAGKLLSPDLYDYLQAAIGRYKSPEEAFRRFDELATRHVEQLRRLTKQIQDARIARDNDAIKRAINEYDEALEAYIPGLMAMASIYWDMELYSNVEKIFRQSAEFCSEHEVWKLNVAHTFFMQDNHYKEAIRYYEPVVKKNADNLLGVTAIVLANLCVSYIMTSQNEEAEELMRKVEKEEERSSMQDPDKPCFHLCIINLVIGTLYCAKGNYEFGVSRIIKSLEPYDKKLETDTWYYAKRCFLALIENLAKHMIVLKDSSFTEIMAFLNEAEKHGKDIRVVFNEGKHQSRTIASEARMLKKMFLKLRD

Entity 20 polymer

描述 description	Intraflagellar transport protein 57
分子量 formula_weight	51374.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	M,m

MSSKRGGRSSLAKAPEEAVNGEAFAPEASPPPPGDDGDAGGEDGGAPAPPPPPATKGGPVAVGRSLEIQTTPDVCMEMLADKLKLLNYEADFCRKKKPYRKPLSRLYFAVPLANSSEQFFYFTSLATWLLGLAGVELPAPKEFDDPNLTCQNILGAVKKLGFAPPSYHPTKLTVGNGKEVVGVLDGLVDFVLERRHHKYSRPAYGNDGQPEEGVQLDDEAEAAAMEGADELAMPAQNQADDDEEEEGVYVDPGRGDAAGPGTGASAAMDAEKAVLVSKVDPTLWKIELERVAPKLRITIAADSKDWRSHLDEAHQHKEVISKAWPDSKTSLERLRADLNGTLEKLQTREKFLNEQFESLMQQYRAARTTFTDVQETYNRKTEAVADRNQEMHRIGETLEEVKAMMDEKGSNIADATPVARIKTAIKQLNKELHDMEVRIGVVSHTLLQLSLRNKRLLQAQAALSDEEED

▶

7. 引用文献 (1)

▶

分辨率 resolution	15.40 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	5896

fit	/app/data/saxs_computation/ru/8ruy/8ruy.fit
pddf	/app/data/pr_computation/ru/8ruy/8ruy.pr.out
profile	/app/data/saxs_computation/ru/8ruy/8ruy.dat

Structure of IFTA and IFTB in Retrograde Intraflagellar transport trains

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

冷冻电镜 EM 3D

5. 实体与聚合体信息 (20)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)