SAXSdb — 8uk3

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Outer capsid protein VP4

Simian rotavirus A strain RRV

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 21	Outer capsid glycoprotein VP7 × 18 （P12476） 2-acetamido-2-deoxy-beta-D-glucopyranose × 18 CALCIUM ION × 54	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	G0YZG6_ROTRH
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 1； PDBConstruct 1–776； UniProt 1–776 Author chain 2； PDBConstruct 1–776； UniProt 1–776 Author chain 3； PDBConstruct 1–776； UniProt 1–776

Outer capsid glycoprotein VP7

Simian rotavirus A strain RRV

UniProt P12476

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 21	Outer capsid protein VP4 × 3 （G0YZG6） 2-acetamido-2-deoxy-beta-D-glucopyranose × 18 CALCIUM ION × 54	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VP7_ROTRH
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain a； PDBConstruct 1–326； UniProt 1–326 Author chain b； PDBConstruct 1–326； UniProt 1–326 Author chain c； PDBConstruct 1–326； UniProt 1–326 Author chain d； PDBConstruct 1–326； UniProt 1–326 Author chain e； PDBConstruct 1–326； UniProt 1–326 Author chain f； PDBConstruct 1–326； UniProt 1–326 Author chain g； PDBConstruct 1–326； UniProt 1–326 Author chain h； PDBConstruct 1–326； UniProt 1–326 Author chain i； PDBConstruct 1–326； UniProt 1–326 Author chain j； PDBConstruct 1–326； UniProt 1–326 Author chain k； PDBConstruct 1–326； UniProt 1–326 Author chain l； PDBConstruct 1–326； UniProt 1–326 Author chain m； PDBConstruct 1–326； UniProt 1–326 Author chain n； PDBConstruct 1–326； UniProt 1–326 Author chain o； PDBConstruct 1–326； UniProt 1–326 Author chain p； PDBConstruct 1–326； UniProt 1–326 Author chain q； PDBConstruct 1–326； UniProt 1–326 Author chain r； PDBConstruct 1–326； UniProt 1–326

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8uk3
Deposition date deposition_date	2023-10-12
Structure title title	The rotavirus VP5/VP8 conformational transition permeabilizes membranes to Ca2+ (class 6 reconstruction)
Keywords keywords	;Non-enveloped virus, viral particle, entry, membrane-penetration, rotavirus, VP4, VP5, VP8, calcium, Ca2+, liposome, VIRAL PROTEIN ;; VIRAL PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8uk3__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8uk3__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8uk3__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	73.13 Å
Rg (electron density)	73.14 Å
Total Rg	73.26 Å
Atom count	88254
Residues	5617
Excluded volume	799940 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8uk3__assembly_1__model_1`	21-meric （21）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	Outer capsid protein VP4
Molecular weight formula_weight	86655.6 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	1,2,3
Atom count atom_count	88254

MASLIYRQLLTNSYTVDLSDEIQEIGSTKTQNVTINLGPFAQTGYAPVNWGPGETNDSTTVEPVLDGPYQPTTFNPPVDYWMLLAPTAAGVVVEGTNNTDRWLATILVEPNVTSETRSYTLFGTQEQITIANASQTQWKFIDVVKTTQNGSYSQYGPLQSTPKLYAVMKHNGKIYTYNGETPNVTTKYYSTTNYDSVNMTAFCDFYIIPREEESTCTEYINNGLPPIQNTRNIVPLALSARNIISHRAQANEDIVVSKTSLWKEMQYNRDITIRFKFASSIVKSGGLGYKWSEISFKPANYQYTYTRDGEEVTAHTTCSVNGMNDFNFNGGSLPTDFVISRYEVIKENSYVYVDYWDDSQAFRNMVYVRSLAANLNSVICTGGDYSFALPVGQWPVMTGGAVSLHSAGVTLSTQFTDFVSLNSLRFRFRLTVEEPSFSITRTRVSRLYGLPAANPNNGKEYYEVAGRFSLISLVPSNDDYQTPITNSVTVRQDLERQLGELREEFNALSQEIAMSQLIDLALLPLDMFSMFSGIKSTIDAAKSMATSVMKKFKKSGLANSVSTLTDSLSDAASSISRGASIRSVGSSASAWTDVSTQITDVSSSVSSISTQTSTISRRLRLKEMATQTEGMNFDDISAAVLKTKIDRSTQISPNTLPDIVTEASEKFIPNRAYRVINNDEVFEAGTDGRFFAYRVETFDEIPFDVQKFADLVTDSPVISAIIDFKTLKNLNDNYGISRQQAFNLLRSDPRVLREFINQDNPIIRNRIEQLIMQCRL

Source Source

Source organism organism	Simian rotavirus A strain RRV
NCBI taxonomy ID ncbi_taxonomy_id	444185
Host organism host_organism	Chlorocebus aethiops

Entity 2 Entity 2 polymer

Description description	Outer capsid glycoprotein VP7
Molecular weight formula_weight	37136.5 kDa
Source method src_method	man
Copy count entity_count	18

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	f,g,h,i,j,k,l,m,n,o,p,q,r,a,b,c,d,e

MYGIEYTTVLTFLISLILLNYILKSLTRMMDFIIYRFLFIVVILSPLLKAQNYGINLPITGSMDTAYANSTQEETFLTSTLCLYYPTEAATEINDNSWKDTLSQLFLTKGWPTGSVYFKEYTDIASFSVDPQLYCDYNVVLMKYDATLQLDMSELADLILNEWLCNPMDITLYYYQQTDEANKWISMGSSCTIKVCPLNTQTLGIGCLTTDTATFEEVATAEKLVITDVVDGVNHKLDVTTATCTIRNCKKLGPRENVAVIQVGGSDVLDITADPTTAPQTERMMRINWKKWWQVFYTVVDYVNQIIQAMSKRSRSLNSAAFYYRI

Source Source

Source organism organism	Simian rotavirus A strain RRV
NCBI taxonomy ID ncbi_taxonomy_id	444185
Host organism host_organism	Chlorocebus aethiops

Entity 3 Entity 3 non-polymer

Description description	2-acetamido-2-deoxy-beta-D-glucopyranose
Molecular weight formula_weight	221.2 kDa
Source method src_method	syn
Copy count entity_count	18

Entity 4 Entity 4 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	54

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	8.00 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	70018

The rotavirus VP5/VP8 conformational transition permeabilizes membranes to Ca2+ (class 6 reconstruction)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Outer capsid protein VP4

State in the Current Structure

Other States of the Same Protein in the Database

Outer capsid glycoprotein VP7

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8uk3__assembly_1__model_1

8uk3__assembly_1__model_1 | I(q)

8uk3__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

7. Citations (1)

The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca2+ (class 6 reconstruction)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Outer capsid protein VP4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Outer capsid glycoprotein VP7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8uk3__assembly_1__model_1

8uk3__assembly_1__model_1 | I(q)

8uk3__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

7. Citations (1)

The rotavirus VP5/VP8 conformational transition permeabilizes membranes to Ca2+ (class 6 reconstruction)