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8uq2

Structure of human RyR2-S2808D in the subprimed state

Method: ELECTRON MICROSCOPY Dmax: 270.5 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ryanodine receptor 2

Homo sapiens

UniProt Q92736

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain A； UniProt 1–4967 Chain B； UniProt 1–4967 Chain C； UniProt 1–4967 Chain D； UniProt 1–4967 Mutation：S2808D Peptidyl-prolyl cis-trans isomerase FKBP1B × 4 （P68106） ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.98 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8UQ2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4JKQ Crystal structure of the N-terminal region of the human ryanodine receptor 2 Deposited 2013-03-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–606（606 aa） Fragment：N-terminal region
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；20 mM TRIS-HCl, 150 mM NaCl, 10% glycerol, 7mM mercaptoethanol, 1 mM CHAPS, 0.1% betaine, 100 mM HEPES, 200 mM ammonium formate, 21% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 2.39 Å R-free 0.261
7KL5 Structure of Calmodulin Bound to the Cardiac Ryanodine Receptor (RyR2) at Residues: Phe4246 to Val4271 Deposited 2020-10-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 4246–4275（30 aa） Fragment：Phe4246 to Thr4275
Not recorded CA CALCIUM ION × 5 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；0.2M sodium acetate, 0.1M Tris pH 8.5, 30%(w/v) PEG 4000
Resolution 1.65 Å R-free 0.245
7U9Q Structure of PKA phosphorylated human RyR2 in the closed state Deposited 2022-03-11 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.11 Å
7U9R Structure of PKA phosphorylated human RyR2 in the open state Deposited 2022-03-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.69 Å
7U9T Structure of PKA phosphorylated human RyR2 in the closed state in the presence of Calmodulin Deposited 2022-03-11 Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.68 Å
7U9X Structure of PKA phosphorylated human RyR2-R2474S in the closed state Deposited 2022-03-11 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.58 Å
7U9Z Structure of PKA phosphorylated human RyR2-R2474S in the open state Deposited 2022-03-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
7UA1 Structure of PKA phosphorylated human RyR2-R2474S in the closed state in the presence of ARM210 Deposited 2022-03-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.99 Å
7UA3 Structure of PKA phosphorylated human RyR2-R2474S in the closed state in the presence of Calmodulin Deposited 2022-03-11 Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
7UA4 Structure of PKA phosphorylated human RyR2-R2474S in the open state in the presence of Calmodulin Deposited 2022-03-11 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.93 Å
7UA5 Structure of dephosphorylated human RyR2 in the closed state Deposited 2022-03-11 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.83 Å
7UA9 Structure of dephosphorylated human RyR2 in the open state Deposited 2022-03-11 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.59 Å
8UQ3 Structure of human RyR2-S2808D in the closed state in the presence of ARM210 Deposited 2023-10-23 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.18 Å
8UQ4 Structure of human RyR2-S2808D in the subprimed state in the presence of H2O2/NOC-12/GSH Deposited 2023-10-23 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；30 minute incubation with H2O2/NOC-12/GSH was stopped by flash freezing (vitrobot)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.64 Å
8UQ5 Structure of human RyR2-S2808D in the primed state in the presence of Rapamycin Deposited 2023-10-23 Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；30-minute incubation with rapamycin was stopped by flash freezing (vitrobot)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.96 Å
8UXC Structure of PKA phosphorylated human RyR2-R420Q in the primed state Deposited 2023-11-09 Different mutation/modification Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Mutation：R420Q Mutation：R420Q Mutation：R420Q Mutation：R420Q ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
8UXE Structure of PKA phosphorylated human RyR2-R420Q in the closed state in the presence of ARM210 Deposited 2023-11-09 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
8UXF Structure of PKA phosphorylated human RyR2-R420W in the primed state Deposited 2023-11-09 Different mutation/modification Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.13 Å
8UXG Structure of PKA phosphorylated human RyR2-R420W in the closed state in the presence of ARM210 Deposited 2023-11-09 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.08 Å
8UXH Structure of PKA phosphorylated human RyR2-R420W in the primed state in the presence of calcium Deposited 2023-11-09 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.52 Å
8UXI Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium Deposited 2023-11-09 Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
8UXL Structure of PKA phosphorylated human RyR2-R420W in the primed state in the presence of calcium and calmodulin Deposited 2023-11-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 20 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；0.020 mM Calmodulin was added to the final sample
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.12 Å
8UXM Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium and calmodulin Deposited 2023-11-09 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–4967（4967 aa）
Chain B 1–4967（4967 aa）
Chain C 1–4967（4967 aa）
Chain D 1–4967（4967 aa）
Not recorded CA CALCIUM ION × 20 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；0.020 mM Calmodulin was added to the final sample
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.56 Å

23 other PDB entries and 23 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RYR2_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–4967； UniProt 1–4967 Author chain B； PDBConstruct 1–4967； UniProt 1–4967 Author chain C； PDBConstruct 1–4967； UniProt 1–4967 Author chain D； PDBConstruct 1–4967； UniProt 1–4967

Peptidyl-prolyl cis-trans isomerase FKBP1B

Homo sapiens

UniProt P68106

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain E； UniProt 1–108 Chain F； UniProt 1–108 Chain G； UniProt 1–108 Chain H； UniProt 1–108 Not recorded Ryanodine receptor 2 × 4 （Q92736） ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 2.98 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8UQ2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1C9H CRYSTAL STRUCTURE OF FKBP12.6 IN COMPLEX WITH RAPAMYCIN Deposited 1999-08-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–108（107 aa） Fragment：FKBP12.6
Not recorded RAP RAPAMYCIN IMMUNOSUPPRESSANT DRUG × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；PEG 4000, LITHIUM SULFATE, HEPES BUFFER, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.249
4C02 Crystal structure of human ACVR1 (ALK2) in complex with FKBP12.6 and dorsomorphin Deposited 2013-07-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–108（108 aa）
Not recorded FLC CITRATE ANION × 8 TAK 6-[4-(2-piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine × 1 EDO 1,2-ETHANEDIOL × 13 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；1.8M AMMONIUM CITRATE, pH 7.2
Resolution 2.17 Å R-free 0.198
4IQ2 P21 crystal form of FKBP12.6 Deposited 2013-01-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–108（107 aa）
Mutation：C22V, C76I MLA MALONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；2.0 M malonic acid, pH 7.0, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.251
4IQ2 P21 crystal form of FKBP12.6 Deposited 2013-01-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2–108（107 aa）
Mutation：C22V, C76I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；2.0 M malonic acid, pH 7.0, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.70 Å R-free 0.251
4IQC P3121 crystal form of FKBP12.6 Deposited 2013-01-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–108（107 aa）
Mutation：C22V, C76I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；62% ammonium sulfate, 0.1 M HEPES, pH 7.5, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.90 Å R-free 0.222
4IQC P3121 crystal form of FKBP12.6 Deposited 2013-01-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 2–108（107 aa）
Mutation：C22V, C76I No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；62% ammonium sulfate, 0.1 M HEPES, pH 7.5, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.90 Å R-free 0.222
5HKG Total chemical synthesis, refolding and crystallographic structure of a fully active immunophilin: calstabin 2 (FKBP12.6). Deposited 2016-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–108（107 aa）
Not recorded RAP RAPAMYCIN IMMUNOSUPPRESSANT DRUG × 1 CL CHLORIDE ION × 3 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；16 % PEG 8K, 10% Glycerol, 500 mM KCl, 100 mM Hepes
Resolution 1.50 Å R-free 0.194
5L1D Structure of rabbit RyR2 in complex with FKBP12.6 in a closed state (conformation C1) Deposited 2016-07-29 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 2–108（107 aa）
Chain D 2–108（107 aa）
Chain F 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；1x Protease Inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
Resolution 11.00 Å
5T15 Structural basis for gating and activation of RyR1 (30 uM Ca2+ dataset, all particles) Deposited 2016-08-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 3.60 Å
5T9M Structure of rabbit RyR1 (Ca2+-only dataset, class 1) Deposited 2016-09-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.00 Å
5T9N Structure of rabbit RyR1 (Ca2+-only dataset, class 2) Deposited 2016-09-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 3.80 Å
5T9R Structure of rabbit RyR1 (Ca2+-only dataset, class 3) Deposited 2016-09-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 5.80 Å
5T9S Structure of rabbit RyR1 (Ca2+-only dataset, class 4) Deposited 2016-09-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.20 Å
5T9V Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1) Deposited 2016-09-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.40 Å
5TA3 Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 2) Deposited 2016-09-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.40 Å
5TAL Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1&2) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.30 Å
5TAM Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 4) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.30 Å
5TAN Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.30 Å
5TAP Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, all particles) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.40 Å
5TAQ Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3&4) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.10 Å
5TAS Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 1) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 6.20 Å
5TAT Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 2) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.80 Å
5TAU Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 3) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 6.20 Å
5TAV Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 4) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.80 Å
5TAW Structure of rabbit RyR1 (ryanodine dataset, all particles) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.40 Å
5TAX Structure of rabbit RyR1 (ryanodine dataset, class 1) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 6.60 Å
5TAY Structure of rabbit RyR1 (ryanodine dataset, class 2) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.60 Å
5TAZ Structure of rabbit RyR1 (ryanodine dataset, class 3) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.30 Å
5TB0 Structure of rabbit RyR1 (EGTA-only dataset, all particles) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.40 Å
5TB1 Structure of rabbit RyR1 (EGTA-only dataset, class 1) Deposited 2016-09-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.60 Å
5TB2 Structure of rabbit RyR1 (EGTA-only dataset, class 2) Deposited 2016-09-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.60 Å
5TB3 Structure of rabbit RyR1 (EGTA-only dataset, class 3) Deposited 2016-09-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.70 Å
5TB4 Structure of rabbit RyR1 (EGTA-only dataset, class 4) Deposited 2016-09-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
Resolution 4.50 Å
6JGZ Structure of RyR2 (F/P/Ca2+ dataset) Deposited 2019-02-16 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain C 1–108（108 aa）
Chain E 1–108（108 aa）
Chain G 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
6JH6 Structure of RyR2 (F/A/Ca2+ dataset) Deposited 2019-02-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain C 1–108（108 aa）
Chain E 1–108（108 aa）
Chain G 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
6JHN Structure of RyR2 (F/C/Ca2+ dataset) Deposited 2019-02-18 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–108（108 aa）
Chain D 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6JI0 Structure of RyR2 (F/A/C/Ca2+ dataset) Deposited 2019-02-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–108（108 aa）
Chain D 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6JI8 Structure of RyR2 (F/apoCaM dataset) Deposited 2019-02-20 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–108（108 aa）
Chain E 1–108（108 aa）
Chain H 1–108（108 aa）
Chain K 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6JII Structure of RyR2 (F/A/C/L-Ca2+/apo-CaM-M dataset) Deposited 2019-02-21 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 1–108（108 aa）
Chain D 1–108（108 aa）
Chain G 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 CFF CAFFEINE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6JIU Structure of RyR2 (F/A/C/L-Ca2+/Ca2+CaM dataset) Deposited 2019-02-23 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–108（108 aa）
Chain E 1–108（108 aa）
Chain H 1–108（108 aa）
Chain K 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 12 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6JIY Structure of RyR2 (F/A/C/H-Ca2+/Ca2+CaM dataset) Deposited 2019-02-24 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–108（108 aa）
Chain E 1–108（108 aa）
Chain H 1–108（108 aa）
Chain K 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6JRR Structure of RyR2 (*F/A/C/L-Ca2+ dataset) Deposited 2019-04-05 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–108（108 aa）
Chain D 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6JRS Structure of RyR2 (*F/A/C/L-Ca2+/Ca2+-CaM dataset) Deposited 2019-04-05 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 1–108（108 aa）
Chain E 1–108（108 aa）
Chain H 1–108（108 aa）
Chain K 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 12 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6M2W Structure of RyR1 (Ca2+/Caffeine/ATP/CaM1234/CHL) Deposited 2020-03-01 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 2–108（107 aa）
Chain E 2–108（107 aa）
Chain H 2–108（107 aa）
Chain K 2–108（107 aa）
Not recorded CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6PV6 Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots Deposited 2019-07-19 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6W1N Pig Ryanodine Receptor (WT) in 5mM EGTA condition Deposited 2020-03-04 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 2–108（107 aa）
Chain C 2–108（107 aa）
Chain E 2–108（107 aa）
Chain G 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
6WOT Cryo-EM structure of recombinant rabbit Ryanodine Receptor type 1 mutant R164C in complex with FKBP12.6 Deposited 2020-04-25 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
6WOU Cryo-EM structure of recombinant mouse Ryanodine Receptor type 2 mutant R176Q in complex with FKBP12.6 in nanodisc Deposited 2020-04-25 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
6WOV Cryo-EM structure of recombinant mouse Ryanodine Receptor type 2 wild type in complex with FKBP12.6 Deposited 2020-04-25 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.10 Å
6X32 Wt pig RyR1 in complex with apoCaM, EGTA condition (class 1 and 2, closed) Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 2–108（107 aa）
Chain D 2–108（107 aa）
Chain G 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6X33 Wt pig RyR1 in complex with apoCaM, EGTA condition (class 3, open) Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 2–108（107 aa）
Chain D 2–108（107 aa）
Chain G 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6X34 Pig R615C RyR1 EGTA (all classes, open) Deposited 2020-05-21 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 2–107（106 aa）
Chain C 2–107（106 aa）
Chain E 2–107（106 aa）
Chain G 2–107（106 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.70 Å
6X35 Pig R615C RyR1 in complex with CaM, EGTA (class 1, open) Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 2–108（107 aa）
Chain D 2–108（107 aa）
Chain G 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6X36 Pig R615C RyR1 in complex with CaM, EGTA (class 3, closed) Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain A 2–108（107 aa）
Chain D 2–108（107 aa）
Chain G 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.70 Å
7CF9 Structure of RyR1 (Ca2+/CHL) Deposited 2020-06-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 2–108（107 aa）
Chain D 2–108（107 aa）
Chain F 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded CA CALCIUM ION × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.70 Å
7JMF Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 42 - State 6 (S6) Deposited 2020-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–108（108 aa）
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7JMG Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 22 - State 2 (S2) Deposited 2020-07-31 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 2–108（107 aa）
Chain F 2–108（107 aa）
Chain H 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7JMH Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 35 - State 4 (S4) Deposited 2020-07-31 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 2–108（107 aa）
Chain F 2–108（107 aa）
Chain H 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7JMI Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 29 - State 3 (S3) Deposited 2020-07-31 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 2–108（107 aa）
Chain F 2–108（107 aa）
Chain H 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7JMJ Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 37 - State 5 (S5) Deposited 2020-07-31 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 2–108（107 aa）
Chain F 2–108（107 aa）
Chain H 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7M6A High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Chain O 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.36 Å
7M6L High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–108（108 aa）
Chain H 1–108（108 aa）
Chain J 1–108（108 aa）
Chain O 1–108（108 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.98 Å
7T64 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the closed state Deposited 2021-12-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging
Resolution 4.00 Å
7T65 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the open state Deposited 2021-12-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging
Resolution 4.05 Å
7U9Q Structure of PKA phosphorylated human RyR2 in the closed state Deposited 2022-03-11 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.11 Å
7U9R Structure of PKA phosphorylated human RyR2 in the open state Deposited 2022-03-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.69 Å
7U9T Structure of PKA phosphorylated human RyR2 in the closed state in the presence of Calmodulin Deposited 2022-03-11 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.68 Å
7U9X Structure of PKA phosphorylated human RyR2-R2474S in the closed state Deposited 2022-03-11 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.58 Å
7U9Z Structure of PKA phosphorylated human RyR2-R2474S in the open state Deposited 2022-03-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
7UA1 Structure of PKA phosphorylated human RyR2-R2474S in the closed state in the presence of ARM210 Deposited 2022-03-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.99 Å
7UA3 Structure of PKA phosphorylated human RyR2-R2474S in the closed state in the presence of Calmodulin Deposited 2022-03-11 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
7UA4 Structure of PKA phosphorylated human RyR2-R2474S in the open state in the presence of Calmodulin Deposited 2022-03-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.93 Å
7UA5 Structure of dephosphorylated human RyR2 in the closed state Deposited 2022-03-11 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.83 Å
7UA9 Structure of dephosphorylated human RyR2 in the open state Deposited 2022-03-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.59 Å
7VML Structure of recombinant RyR2 (EGTA dataset, class 1&2, closed state) Deposited 2021-10-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Chain I 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7VMM Structure of recombinant RyR2 (EGTA dataset, class 1, closed state) Deposited 2021-10-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Chain I 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7VMN Structure of recombinant RyR2 (EGTA dataset, class 2, closed state) Deposited 2021-10-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Chain I 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7VMO Structure of recombinant RyR2 (Ca2+ dataset, class 1, open state) Deposited 2021-10-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Chain I 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7VMP Structure of recombinant RyR2 (Ca2+ dataset, class 2, open state) Deposited 2021-10-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Chain I 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7VMQ Structure of recombinant RyR2 (Ca2+ dataset, class 3, open state) Deposited 2021-10-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Chain I 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7VMR Structure of recombinant RyR2 mutant K4593A (EGTA dataset) Deposited 2021-10-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Chain I 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7VMS Structure of recombinant RyR2 mutant K4593A (Ca2+ dataset) Deposited 2021-10-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Chain I 2–108（107 aa）
Chain J 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8DTY Recombinant mouse RyR2 triple phosphomimetic mutant S2807D/S2813D/S2030D in complex with FKBP12.6 and nanodisc under closed-state conditions Deposited 2022-07-26 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8DTZ Recombinant mouse RyR2 triple phosphonull mutant S2807A/S2813A/S2030A in complex with FKBP12.6 and nanodisc under closed-state conditions Deposited 2022-07-26 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8DUJ Global map in C1 of RyR1 particles in complex with ImperaCalcin Deposited 2022-07-27 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain B 2–108（107 aa）
Chain E 2–108（107 aa）
Chain H 2–108（107 aa）
Chain K 2–108（107 aa）
Not recorded CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8DVE RyR1 in presence of IpCa-T26E phosphomimetic and activating ligands Deposited 2022-07-28 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 2–108（107 aa）
Chain E 2–108（107 aa）
Chain H 2–108（107 aa）
Chain K 2–108（107 aa）
Not recorded CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.84 Å
8DVV Recombinant mouse RyR2 triple phosphomimetic mutant S2807D/S2813D/S2030D in complex with FKBP12.6 and nanodisc under open-state conditions Deposited 2022-07-29 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded CA CALCIUM ION × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.68 Å
8SEN Cryo-EM Structure of RyR1 Deposited 2023-04-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
8SEO Cryo-EM Structure of RyR1 + ATP-gamma-S Deposited 2023-04-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.92 Å
8SEP Cryo-EM Structure of RyR1 + ADP Deposited 2023-04-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.57 Å
8SEQ Cryo-EM Structure of RyR1 + AMP Deposited 2023-04-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded AMP ADENOSINE MONOPHOSPHATE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8SER Cryo-EM Structure of RyR1 + Adenosine Deposited 2023-04-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ADN ADENOSINE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.42 Å
8SES Cryo-EM Structure of RyR1 + Adenine Deposited 2023-04-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ADE ADENINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.98 Å
8SET Cryo-EM Structure of RyR1 + cAMP Deposited 2023-04-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.42 Å
8UQ3 Structure of human RyR2-S2808D in the closed state in the presence of ARM210 Deposited 2023-10-23 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.18 Å
8UQ4 Structure of human RyR2-S2808D in the subprimed state in the presence of H2O2/NOC-12/GSH Deposited 2023-10-23 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；30 minute incubation with H2O2/NOC-12/GSH was stopped by flash freezing (vitrobot)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.64 Å
8UXC Structure of PKA phosphorylated human RyR2-R420Q in the primed state Deposited 2023-11-09 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
8UXE Structure of PKA phosphorylated human RyR2-R420Q in the closed state in the presence of ARM210 Deposited 2023-11-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.53 Å
8UXF Structure of PKA phosphorylated human RyR2-R420W in the primed state Deposited 2023-11-09 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.13 Å
8UXG Structure of PKA phosphorylated human RyR2-R420W in the closed state in the presence of ARM210 Deposited 2023-11-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.08 Å
8UXH Structure of PKA phosphorylated human RyR2-R420W in the primed state in the presence of calcium Deposited 2023-11-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.52 Å
8UXI Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium Deposited 2023-11-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.29 Å
8UXL Structure of PKA phosphorylated human RyR2-R420W in the primed state in the presence of calcium and calmodulin Deposited 2023-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 20 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；0.020 mM Calmodulin was added to the final sample
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.12 Å
8UXM Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium and calmodulin Deposited 2023-11-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 1–108（108 aa）
Chain F 1–108（108 aa）
Chain G 1–108（108 aa）
Chain H 1–108（108 aa）
Not recorded CA CALCIUM ION × 20 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；0.020 mM Calmodulin was added to the final sample
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.56 Å
8XJI Structure of chimeric RyR complex with flubendiamide Deposited 2023-12-21 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded A1LVX Flubendiamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.91 Å
8XKH Structure of chimeric RyR Complex with tetraniliprole Deposited 2023-12-23 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 A1LV1 tetraniliprole × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.87 Å
8XLF Structure of chimeric RyR Deposited 2023-12-25 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded CA CALCIUM ION × 4 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
8XLH Structure of chimeric RyR-I4657M/G4819E Deposited 2023-12-26 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
8Y40 Structure of chimeric RyR-I4657M/G4819E complex with chlorantraniliprole Deposited 2024-01-29 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.58 Å
9C1E Mink RyR3 in closed conformation Deposited 2024-05-29 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 2–108（107 aa）
Chain C 2–108（107 aa）
Chain E 2–108（107 aa）
Chain G 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CL CHLORIDE ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES pH 7.5, 250 mM NaCl, 2 mM DTT, 0.02% GDN, 5 mM EGTA, 1:1000 diluted protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；blotted for 3 s (blot force of 7 to 10) using ashless blotting paper (Whatman)
Resolution 2.89 Å
9C1F Mink RyR3 in open conformation bound to Ca2+/ATP/caffeine Deposited 2024-05-29 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 2–108（107 aa）
Chain C 2–108（107 aa）
Chain E 2–108（107 aa）
Chain G 2–108（107 aa）
Not recorded ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CFF CAFFEINE × 4 CL CHLORIDE ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES pH 7.5, 250 mM NaCl, 2 mM DTT, 0.02% GDN, 0.03 mM free Ca2+ buffered with 2 mM EGTA, 5 mM ATP, 5 mM caffeine, 1:1000 diluted protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；blotted for 3 s (blot force of 7 to 10) using ashless blotting paper (Whatman)
Resolution 3.22 Å
9CGP RyR1 disease mutant Y523S with FKBP12.6, nanodisc and inhibitor dantrolene in the absence of calcium with refined P1 domain Deposited 2024-06-30 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 U1C Dantrolene × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.34 Å
9CGQ Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6, nanodisc and inhibitor dantrolene in the presence of activating calcium Deposited 2024-06-30 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 2–108（107 aa）
Chain F 2–108（107 aa）
Chain G 2–108（107 aa）
Chain H 2–108（107 aa）
Not recorded ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 U1C Dantrolene × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.23 Å
9KDF CryoEM structure of Calcineurin-fusion Human endothelin receptor type-B in complex with RES-701-3 Deposited 2024-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 2–108（107 aa）
Not recorded CA CALCIUM ION × 4 FE FE (III) ION × 1 ZN ZINC ION × 1 FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9KDG CryoEM structure of Calcineurin-fusion Human endothelin receptor type-B in the ligand-free form Deposited 2024-11-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 2–108（107 aa）
Not recorded CA CALCIUM ION × 4 FE FE (III) ION × 1 ZN ZINC ION × 1 FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.33 Å
9OL4 Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation Deposited 2025-05-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–108（108 aa）
Chain H 1–108（108 aa）
Chain N 1–108（108 aa）
Chain T 1–108（108 aa）
Not recorded CFF CAFFEINE × 4 CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 12 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.37 Å
9OL5 Rabbit Ryanodine Receptor 1: Atorvastatin Bound Closed Conformation Deposited 2025-05-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–108（108 aa）
Chain H 1–108（108 aa）
Chain N 1–108（108 aa）
Chain T 1–108（108 aa）
Not recorded CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.56 Å
9OL6 Rabbit Ryanodine Receptor 1: DMSO Control Closed Conformation Deposited 2025-05-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–108（108 aa）
Chain H 1–108（108 aa）
Chain N 1–108（108 aa）
Chain T 1–108（108 aa）
Not recorded CA CALCIUM ION × 4 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.11 Å
9PWO Pig Ryanodine Receptor 1 R615C Mutant: Atorvastatin Bound Open Conformation Deposited 2025-08-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–108（108 aa）
Chain H 1–108（108 aa）
Chain N 1–108（108 aa）
Chain T 1–108（108 aa）
Not recorded CFF CAFFEINE × 4 CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 12 ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.36 Å

117 other PDB entries and 119 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name FKB1B_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain E； PDBConstruct 1–108； UniProt 1–108 Author chain F； PDBConstruct 1–108； UniProt 1–108 Author chain G； PDBConstruct 1–108； UniProt 1–108 Author chain H； PDBConstruct 1–108； UniProt 1–108

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8uq2

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8uq2
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8uq2
Deposition date deposition_date2023-10-23
Structure title titleStructure of human RyR2-S2808D in the subprimed state
Keywords keywordscalcium channel, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier— Å
Radius of gyration Rg (electron density) rg_electron104.10 Å
Forward intensity I(0) i052483100000.00
Molecular weight molecular_weight1972400.0 kDa
Excluded volume excluded_volume2472100 ų
Envelope volume envelope_volume4555900 ų
Hydration-shell volume shell_volume357930 ų
Envelope diameter envelope_diameter375.5 Å
Shell Rg shell_rg104.70 Å
Envelope Rg envelope_rg100.50 Å
Shape Rg shape_rg104.10 Å
Total Rg total_rg104.00 Å
Total atoms total_atoms138576
Residues n_residues17324
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax270.5 Å
Rg (real space) rg_real100.00 Å
Rg uncertainty (real space) rg_real_error0.67 Å
I(0) (real space) i0_real5.0170e+10
I(0) uncertainty (real space) i0_real_error9.0680e+08
Rg (reciprocal space) rg_reciprocal104.50 Å
I(0) (reciprocal space) i0_reciprocal52510000000.0000
Solution quality estimate total_estimate0.9096
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary126.2 Å
Skewness Skewness skewness0.110
Kurtosis Kurtosis kurtosis-0.659
Angular range angular_range— – 0.0750 Å−1
Current regularization parameter α current_alpha0.7074
Highest regularization parameter α highest_alpha3825000000.0000
Real-space data points n_real_points16
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.001; Oscil: 0.999; Stabil: 0.958; Sysdev: 1.000; Positv: 1.000; Valcen: 0.952; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (4)

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8. Citations (1)

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9. Files and Curves (10)