8vjh

Cryo-EM of tail-tip of bacteriophage Chi

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Minor tail protein

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 7	Distal tail protein × 1 （M9NT03） Baseplate hub 1 protein, gp27 × 1 （M9NUT0） Tail Tube × 2 （M9NUS9） Tape measure × 1 （M9NVD0） Tail assembly protein 1 × 1 （M9NTL4） FE (III) ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	M9NVD3_9CAUD
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–1296； UniProt 1–1296

Distal tail protein

OrganismNot specified

UniProt M9NT03

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 7	Minor tail protein × 1 （M9NVD3） Baseplate hub 1 protein, gp27 × 1 （M9NUT0） Tail Tube × 2 （M9NUS9） Tape measure × 1 （M9NVD0） Tail assembly protein 1 × 1 （M9NTL4） FE (III) ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	M9NT03_9CAUD
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–562； UniProt 1–562

Baseplate hub 1 protein, gp27

OrganismNot specified

UniProt M9NUT0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 7	Minor tail protein × 1 （M9NVD3） Distal tail protein × 1 （M9NT03） Tail Tube × 2 （M9NUS9） Tape measure × 1 （M9NVD0） Tail assembly protein 1 × 1 （M9NTL4） FE (III) ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	M9NUT0_9CAUD
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain D； PDBConstruct 1–272； UniProt 1–272

Tail Tube

OrganismNot specified

UniProt M9NUS9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 7	Minor tail protein × 1 （M9NVD3） Distal tail protein × 1 （M9NT03） Baseplate hub 1 protein, gp27 × 1 （M9NUT0） Tape measure × 1 （M9NVD0） Tail assembly protein 1 × 1 （M9NTL4） FE (III) ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	M9NUS9_9CAUD
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain J； PDBConstruct 1–381； UniProt 1–381 Author chain K； PDBConstruct 1–381； UniProt 1–381

Tape measure

OrganismNot specified

UniProt M9NVD0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 7	Minor tail protein × 1 （M9NVD3） Distal tail protein × 1 （M9NT03） Baseplate hub 1 protein, gp27 × 1 （M9NUT0） Tail Tube × 2 （M9NUS9） Tail assembly protein 1 × 1 （M9NTL4） FE (III) ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	M9NVD0_9CAUD
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain Q； PDBConstruct 1–1431； UniProt 1–1431

Tail assembly protein 1

OrganismNot specified

UniProt M9NTL4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 7	Minor tail protein × 1 （M9NVD3） Distal tail protein × 1 （M9NT03） Baseplate hub 1 protein, gp27 × 1 （M9NUT0） Tail Tube × 2 （M9NUS9） Tape measure × 1 （M9NVD0） FE (III) ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	M9NTL4_9CAUD
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain U； PDBConstruct 1–76； UniProt 1–76

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8vjh
Deposition date deposition_date	2024-01-06
Last revision last_revision	2024-10-02
Structure title title	Cryo-EM of tail-tip of bacteriophage Chi
Keywords keywords	Flagellotropic bacteriophage, Siphophage, Tail-tip, VIRUS; VIRUS
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8vjh__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8vjh__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8vjh__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	63.52 Å
Rg (electron density)	61.29 Å
Total Rg	60.02 Å
Atom count	15967
Residues	2219
Excluded volume	276470 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8vjh__assembly_1__model_1`	heptameric （7）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Minor tail protein
Molecular weight formula_weight	143342.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	15967

MGSKSKKVTVGYKYYMGLFMGLFRGPVNEITEIRVGDRTAWTGSITGNTTIQINKESLFGGTKAEGGIDGPLEVYMGAPTQTVSQKLKNMLGGRQPEFRGVVTAYFDGLICAMNPYPKQWKFKARRSTAGWTGGVWYPEKCLVKMQGYDGQGNQHEIHAMNPAHILYECQSNYEWGRGLSRTLIDDTTFRLAADTLFNENFGLCIRWNRQDTLESFMQLILDHIGGAMYVSKVTGKLSLRLIRKDYDFDTLPIFDTDSGLLSIQEATNASPANLVNEVVVTYHNPVMDEDQQVRSHNLAQIQNQGCLNSNTIEYLGIPTGKLAMQVAQRDLRAASTNVRRFTVICDRRAWNVQPGDVLKVRDPKQRGLTEVVIRVGTVEDGTLPDGKIKVVALQDQFAFQLNTFNQVEPPAGYEPNLTPAIARRIVYEMPYVDLVQQLPDGELNAVGPNDAFINSQAEKPTDMSAAYDMGIMAEGESGFDVRGNGDFGAFGSLASDIDYLSTQIVLSEMTDMWDDVQPGFVARIAKPVLDGQRTQMEIAEEFVRIDAINGNVITVARGVLDTIPFRHQKSEMLWVTTYDGGTDWQKYAGNESIDIKILPWTLGGGRFPIEDAPIDHLDMDFRQIRPYPPGNVQHYLASTSTLQRWYVPSALTYTANAGETPDTYTLTWAHRDRVLQGDKPVPHIDGDIGPEPGTSYTIRVYNQEGTLVRTESGINGTTWNWPYATAAADMNVEASLFDPVLATLRLTSARNGRESWEYYEMKVSVYKKPPQFVYDASLMQFAAQPYNATSDPNPPTPPMDGPYVSSLMQFAAQPDGSEDFGPAESMNGPNVALLPHQVTQTSSIITPLDTLLYETPYIQLSRDGRDLNTSKVSAYVARSSDRTVDSYTLFTKHETDANYTSSGTQPWTPWGMSTVGLGFFTDEVTIDPTSDKDGVPVAPAKVGDLLLIDQELVSITAINGRTYKIGRGVADTIPAQHYSSRPVWLISVGFGYSDMAFGDNEKAMVIIRPDTYGTDIPLSKLYPLQLQMQYRPKRPYPPGLMMIGGQPFFNTASGLADDFNPYDNLKAKDVVCTYAHRNRVSQSTTARDHFAVGITPEPGVKYRVRIGYAYWSQSGSGFSLLSQFETEDAGFIMRAADLERWGRQAGYAQDAGGYSTLNVTVNAIRDGMLNWQGYTMTVRVPSFPLPPGQKPGGGDGPWNPPGGGNTGGTTPPTEPPDRPDPSEPGTTDPSEPTKPDEPEEPQPPVDPEDPDTDPPEPPEPPVDPTNVPGWSLSWDHGWAMTLPDFRYVPPADEEPE

Entity 2 Entity 2 polymer

Description description	Distal tail protein
Molecular weight formula_weight	63032.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MALSGLRPAREQFNQAIYDALNGAYQLYVQDAFGQAAANQTYYMAKWMKLKAGTYTAVMYVDDSGTLSIDHAVVATAAIGTNPNSGEFTVAADGVYRFDCIYSNVPADTPAYMAYQLIRDGQTVEVSRANDFIADIVPIPDKALGPKPPYSDDVRLTYPVFLPLPNWKDGVTERIEWQTDVMISESGAEQRRPIRLHPRRSFEATFLRWEENRTLLDTTIAGVGQSPLLLPLWHDMTATENNAPAGSVDIFGQFRVKDFNVGDVVMFNRGTTWDYETNIIAGLDIDAGHMTLTFGLQSDTPKGTRLYPVRVAQIREAMNGQQMTDSVSQTQVRFFCTENYDLTPSWSDFPIYTRTGLHIFVLPEDWGSSNEITSDRLTYNFDNQSGPVVVVDPGGQNYGTVKKSYTIKGRTADRQFRQILFALRGRTKTFHLPLDTNDFILSRDINPADGALVVRRCGYTQYIGGTQETKRDIMVELYDGTRIPTTIISSRIVGDEEWLFLSQSIPATSRNDVRRIGYIPVARLDVDGIEIKRLTDSAGVSQVSLTFKFFDDRRIATPLPLS

Entity 3 Entity 3 polymer

Description description	Baseplate hub 1 protein, gp27
Molecular weight formula_weight	29348.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MSYNIIETSNDNGRPVFMYEFRLLDKYWRYTSADAKVSALGSIWEPMGVSDDGIKQTGEAKTDALNLTLPNSNPVVGLFIGTPPGSPVTLTIRRMHLDDNDPVVCYVGTVDSINQGENPTVATVTCSTLSATMDRNGLRLSWSRGCPHALYDGQCRVNKEAFRVDATILTVGAGTVTAAAYATRPDGYFAGGFIEWIDPVYGVERRGIETHTGNTITIFGTVDGLAGGYILKTYPGCPRTSAACDTIFNNLANFGGIPSLPDRSPFDGNPIF

Entity 4 Entity 4 polymer

Description description	Tail Tube
Molecular weight formula_weight	40375.4 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J,K

MNDNYQNNYVVGRGTVYFDRFQDGTNRKTGEMYFGNTPEFTINTDSETLDHYSSDHGMRVMDASVLLEASQGGTFTCDNINADNLALWFLGEVSNTTQTQQTDAKEVFNPIMRGRYYQLGTTDDNPTGVRGVTNFQMVKADASIAISVGSGDITSIVGATVVNPAGNYEIDLEAGRIYIEPDSTDLSGNVQIAVQYDVDAQKRTLVIGKSNMVYGALRMISDNPVGLNKNYYFPKVSIAPDGDYALKGDDWQVMSFTFKAMQLNNITQRVYIDIVEAAAAVDPTAQRTIEITPASTTATTGGAGVVCTVTVRDGTGTAVQGDAVTFTTVAGATVTPNSATTGATGTATTTVNRAAAGTATVTATLANGKAATTGTITFSAP

Entity 5 Entity 5 polymer

Description description	Tape measure
Molecular weight formula_weight	153960.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MANSKDVELRIRARDYSQKPLKAVTSAIEQMAKAQDEQRKAAERGEISTRDLEAAYKKLESAGSQLLKLNSLIEVFKRQNQAMTEATAKTEGLRQKQADLQKTYDSTEKVTQKQERALARVTRQVEAATRAEANQAERVNRATRDLERYGIETSKVGAAQAGIVTSVAQVNRVLERQDEIISTSAAAAAQAKVIRGLQQQADQAMATARGYQTLGRVVQQATGQLGPLGTQIQTIVSPAEAARRTLSGLENQVHGVTAELARNSKEVENVAQKVRMLNEANKTVSALAQQIDLYRQQVATLRNARTEYQQARQDVIKLAQQMRTATTDTGALGIQMQAAQQRLSAAATAMRNTATAARSTQAALRAAQVDTRNLSDAEARLISTSQQSAAALNTLSTATNRNSQAARDGSKAWSLFRDEGRTTLSFLQRIRGEVLALTTTYVGFQGAISLAGGAIDAYKNRQQAMVKIANVVGNSQAAINKEWEYMVGLANTLGIDITTLSQSYTKFAVSAKAVGLSLQDSKFIFESVAKAGRVFHLSQDDMEGVFRALEQMLSKGQVYAEELRGQLGERLPAAFALFAKGMDMTTAELMKAMENGEVTGEAVINFAREQAKAIDAQLATAQKGVDAMEARARNAMNAFQLALADAGFIEAYVQMLNKITDFLNSEDGRAAAVKLGEAFGMLADAVTWCIENVDTLITALSILAGLKVVQFIGGMISGLKNLLPLFNTLSKIGAGIITVLEGVAARMIAAEGAIGLLGVALKGLTRLIPIVGWALLAYDIGAIMYDQSQTFREAVNAVIRDFKNLGNQLAAVVDTIPALLYDLAVSVLRPITTMFADTTQAIIKWIADVLKLIPGVGQGLSEWATSIGDDLTKEQRGFLESTGRIWDNVNKQWVKLNDDMVAKNADATDKIRGQVNQLAADMAAITKGEGFQFTQDPGTGVTKRSREIAGLTKELNKMEEAAKKADVASRKAEQRKNLSGRLAIIDEEFAPQYARAKSIGGSEGDALTKRLDAVVAARKKAETTLFNSQQRTTGGIKKQENALQALINKYNELNAAVGVKEVKIDPNATFDDRLAAKLAAVNTQYDQLIAKSKKLGTGGENLAGQFEDLRKRNLEYATTQAKLEELKRIQDQLNAQQETRKNLLDEINAKRQAGIISEDEAVSQTVALYQNMNAGIASSAEQLDVFAQKIKDTMSPEEFSRIMAQIAAVKAGLVDVTGTFTTMDTTVVQGVLDGMSTALSSIVDEMALVVAGSQSIGDAFSNLGVAVARFFADFLQKIAMAILQQMALNALASMGGGIGSAAVALGGTVAKHNGGTVGSKTTGGTQMKGGISPAMFANAPRFHDGGLPGLRSDEVPTILQKGEQVLSKDDPNNVLNQSRGGGQTAQSPQGLRFVLVDDRSKVPEAMNTPEGEKAVMQILQRNVPTLKNLVG

Entity 6 Entity 6 polymer

Description description	Tail assembly protein 1
Molecular weight formula_weight	8278.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MWWALAMLVASVLINAALAPKPASAKPATIQDFDIPQVKEGTPQSVVFGEVWTADWQVLGFGNFRTKAVKAKQAKK

Entity 7 Entity 7 non-polymer

Description description	FE (III) ION
Molecular weight formula_weight	55.8 kDa
Source method src_method	syn
Copy count entity_count	1

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	4.20 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	2159

Cryo-EM of tail-tip of bacteriophage Chi

1. Protein Identity and Related Structures Protein Identity & Related Structures

Minor tail protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Distal tail protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Baseplate hub 1 protein, gp27

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Tail Tube

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Tape measure

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Tail assembly protein 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8vjh__assembly_1__model_1

8vjh__assembly_1__model_1 | I(q)

8vjh__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

Entity 5 Entity 5 polymer

Polymer Polymer

Entity 6 Entity 6 polymer

Polymer Polymer

Entity 7 Entity 7 non-polymer

7. Citations (1)