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9ct0

Native human GABAA receptor of beta2-alpha1-beta2-alpha2-gamma2 assembly

Method: ELECTRON MICROSCOPY Dmax: 130.6 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Gamma-aminobutyric acid receptor subunit beta-2

OrganismNot specified

UniProt P47870

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 7 其他Polymer 5 PDB declaration: heptameric（7） Consistent with protein copy count Chain A； UniProt 25–512 Chain C； UniProt 25–512 Not recorded Gamma-aminobutyric acid receptor subunit alpha-1 × 1 （P14867） Gamma-aminobutyric acid receptor subunit alpha-2 × 1 （P47869） Gamma-aminobutyric acid receptor subunit gamma-2 × 1 （P18507） Kappa Fab_1F4 Light Chain × 1 IgG2b Fab_1F4 Heavy Chain × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE；3.5 s Blot. Resolution 3.19 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CT0 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6D6T Human GABA-A receptor alpha1-beta2-gamma2 subtype in complex with GABA and flumazenil, conformation B Deposited 2018-04-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 486–512（27 aa）
Chain C 25–331（307 aa）
Chain C 486–512（27 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 Y01 CHOLESTEROL HEMISUCCINATE × 12 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 seconds blot time
Resolution 3.86 Å
6D6U Human GABA-A receptor alpha1-beta2-gamma2 subtype in complex with GABA and flumazenil, conformation A Deposited 2018-04-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 486–512（27 aa）
Chain C 25–331（307 aa）
Chain C 486–512（27 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 Y01 CHOLESTEROL HEMISUCCINATE × 12 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 seconds blot time
Resolution 3.92 Å
6X3S Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with bicuculline methbromide Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 J94 (5S)-6,6-dimethyl-5-[(6R)-8-oxo-6,8-dihydrofuro[3,4-e][1,3]benzodioxol-6-yl]-5,6,7,8-tetrahydro[1,3]dioxolo[4,5-g]isoquinolin-6-ium × 2 MAN alpha-D-mannopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.12 Å
6X3T Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus propofol Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.55 Å
6X3U Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus flumazenil Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.50 Å
6X3V Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus etomidate Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.50 Å
6X3W Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus phenobarbital Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 UQA 5-ethyl-5-phenylpyrimidine-2,4,6(1H,3H,5H)-trione × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.30 Å
6X3X Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus diazepam Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.92 Å
6X3Z Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.23 Å
6X40 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus picrotoxin Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.86 Å
7T0W Complex of GABA-A synaptic receptor with autoimmune antibody Fab115 Deposited 2021-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 488–511（24 aa）
Chain C 25–331（307 aa）
Chain C 488–511（24 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7T0Z Complex of GABA-A synaptic receptor with autoimmune antibody Fab175 Deposited 2021-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 25–331（307 aa）
Chain A 488–511（24 aa）
Chain C 25–331（307 aa）
Chain C 488–511（24 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 MAN alpha-D-mannopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8DD2 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus Zolpidem Deposited 2022-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 486–511（26 aa）
Chain C 25–331（307 aa）
Chain C 486–511（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R5R Zolpidem × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.90 Å
8DD3 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus DMCM Deposited 2022-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 486–511（26 aa）
Chain C 25–331（307 aa）
Chain C 486–511（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R63 methyl 4-ethyl-6,7-dimethoxy-9H-pyrido[3,4-b]indole-3-carboxylate × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.90 Å
8SGO Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus pregnenolone sulfate Deposited 2023-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 486–512（27 aa）
Chain C 25–331（307 aa）
Chain C 486–512（27 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PTY PHOSPHATIDYLETHANOLAMINE × 6 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 1 A8W Pregnenolone sulfate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
8SI9 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus allopregnanolone Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 486–512（27 aa）
Chain C 25–331（307 aa）
Chain C 486–512（27 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 Y4B allopregnanolone × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.98 Å
8SID Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus dehydroepiandrosterone sulfate Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 486–512（27 aa）
Chain C 25–331（307 aa）
Chain C 486–512（27 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ZWY 17-oxoandrost-5-en-3beta-yl hydrogen sulfate × 1 PTY PHOSPHATIDYLETHANOLAMINE × 6 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 1 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.71 Å
8VQY Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus methaqualone Deposited 2024-01-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain C 25–331（307 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 A1ADG 2-methyl-3-(2-methylphenyl)quinazolin-4(3H)-one × 3 PTY PHOSPHATIDYLETHANOLAMINE × 4 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
8VRN Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus PPTQ Deposited 2024-01-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain C 25–331（307 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 A1ADN 3-(4-methylphenyl)-2-phenylquinazolin-4(3H)-one × 2 PTY PHOSPHATIDYLETHANOLAMINE × 5 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.57 Å
9CRS Native human GABAA receptor of beta2-alpha1-beta2-alpha1-gamma2 assembly Deposited 2024-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–512（488 aa）
Chain C 25–512（488 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5s blot
Resolution 2.90 Å
9CRV Native human GABAA receptor of beta2-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 25–512（488 aa）
Chain D 25–512（488 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5s blot
Resolution 3.18 Å
9CSB Native human GABAA receptor of beta3-alpha1-beta2-alpha2-gamma2 assembly Deposited 2024-07-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–512（488 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.34 Å
9CTJ Native human GABAA receptor of beta2-alpha1-beta3-alpha2-gamma2 assembly Deposited 2024-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 25–512（488 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.74 Å
9CTP Native human GABAA receptor of beta2-alpha1-beta2-alpha3-gamma2 assembly Deposited 2024-07-25 Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 26–512（487 aa）
Chain C 26–512（487 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.62 Å
9CTV Native human GABAA receptor of beta2-alpha1-gamma2-beta1-alpha2 assembly Deposited 2024-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 26–512（487 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.36 Å
9CXA Native human GABAA receptor of beta2-alpha1-beta3-alpha1-gamma2 assembly Deposited 2024-07-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 1–512（512 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
9CXB Native human GABAA receptor of beta2-alpha1-beta1-alpha2-gamma2 assembly Deposited 2024-07-31 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 25–512（488 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.33 Å
9CXC Native human GABAA receptor of beta3-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 25–512（488 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.30 Å
9CXD Native human GABAA receptor of beta2-alpha1-beta1-beta1-gamma2 assembly Deposited 2024-07-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 26–512（487 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.36 Å
9DRX Human GABAA receptor of beta2-alpha1-beta2-alpha1-gamma2 subtype in complex with GABA plus Lamotrigine Deposited 2024-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 25–331（307 aa）
Chain A 487–512（26 aa）
Chain C 25–331（307 aa）
Chain C 487–512（26 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 IYJ (6M)-6-(2,3-dichlorophenyl)-1,2,4-triazine-3,5-diamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 2.95 Å

30 other PDB entries and 30 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name GBRB2_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–488； UniProt 25–512 Author chain C； PDBConstruct 1–488； UniProt 25–512

Gamma-aminobutyric acid receptor subunit alpha-1

OrganismNot specified

UniProt P14867

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 7 其他Polymer 5 PDB declaration: heptameric（7） Consistent with protein copy count Chain B； UniProt 28–456 Not recorded Gamma-aminobutyric acid receptor subunit beta-2 × 2 （P47870） Gamma-aminobutyric acid receptor subunit alpha-2 × 1 （P47869） Gamma-aminobutyric acid receptor subunit gamma-2 × 1 （P18507） Kappa Fab_1F4 Light Chain × 1 IgG2b Fab_1F4 Heavy Chain × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE；3.5 s Blot. Resolution 3.19 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CT0 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6CDU Crystal structure of a chimeric human alpha1GABAA receptor in complex with alphaxalone Deposited 2018-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric
Chain A 249–340（92 aa）
Chain A 415–444（30 aa）
Chain B 249–340（92 aa）
Chain B 415–444（30 aa）
Chain C 249–340（92 aa）
Chain C 415–444（30 aa）
Chain D 249–340（92 aa）
Chain D 415–444（30 aa）
Chain E 249–340（92 aa）
Chain E 415–444（30 aa）
Not recorded EY4 (3a,5a)-3-Hydroxypregnane-11,20-dione × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.1；277 K；PEG400, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, NaSCN
Resolution 3.45 Å R-free 0.286
6CDU Crystal structure of a chimeric human alpha1GABAA receptor in complex with alphaxalone Deposited 2018-02-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric
Chain F 249–340（92 aa）
Chain F 415–444（30 aa）
Chain G 249–340（92 aa）
Chain G 415–444（30 aa）
Chain H 249–340（92 aa）
Chain H 415–444（30 aa）
Chain I 249–340（92 aa）
Chain I 415–444（30 aa）
Chain J 249–340（92 aa）
Chain J 415–444（30 aa）
Not recorded EY4 (3a,5a)-3-Hydroxypregnane-11,20-dione × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.1；277 K；PEG400, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, NaSCN
Resolution 3.45 Å R-free 0.286
6D1S Crystal structure of an apo chimeric human alpha1GABAA receptor Deposited 2018-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric
Chain A 249–340（92 aa）
Chain A 415–444（30 aa）
Chain B 249–340（92 aa）
Chain B 415–444（30 aa）
Chain C 249–340（92 aa）
Chain C 415–444（30 aa）
Chain D 249–340（92 aa）
Chain D 415–444（30 aa）
Chain E 249–340（92 aa）
Chain E 415–444（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.1；277.1 K；100 mM 2-(N-morpholino)ethanesulfonic acid, 400mM NaSCN, 21% PEG400
Resolution 3.20 Å R-free 0.269
6D1S Crystal structure of an apo chimeric human alpha1GABAA receptor Deposited 2018-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric
Chain F 249–340（92 aa）
Chain F 415–444（30 aa）
Chain G 249–340（92 aa）
Chain G 415–444（30 aa）
Chain H 249–340（92 aa）
Chain H 415–444（30 aa）
Chain I 249–340（92 aa）
Chain I 415–444（30 aa）
Chain J 249–340（92 aa）
Chain J 415–444（30 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.1；277.1 K；100 mM 2-(N-morpholino)ethanesulfonic acid, 400mM NaSCN, 21% PEG400
Resolution 3.20 Å R-free 0.269
6D6T Human GABA-A receptor alpha1-beta2-gamma2 subtype in complex with GABA and flumazenil, conformation B Deposited 2018-04-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 Y01 CHOLESTEROL HEMISUCCINATE × 12 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 seconds blot time
Resolution 3.86 Å
6D6U Human GABA-A receptor alpha1-beta2-gamma2 subtype in complex with GABA and flumazenil, conformation A Deposited 2018-04-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 Y01 CHOLESTEROL HEMISUCCINATE × 12 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 seconds blot time
Resolution 3.92 Å
6HUJ CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with picrotoxin, GABA and megabody Mb38. Deposited 2018-10-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
6HUK CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38. Deposited 2018-10-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded H0Z bicuculline methochloride × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.69 Å
6HUO CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with alprazolam (Xanax), GABA and megabody Mb38. Deposited 2018-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 08H 8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.26 Å
6HUP CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with diazepam (Valium), GABA and megabody Mb38. Deposited 2018-10-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.58 Å
6I53 Cryo-EM structure of the human synaptic alpha1-beta3-gamma2 GABAA receptor in complex with Megabody38 in a lipid nanodisc Deposited 2018-11-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–456（456 aa）
Chain D 1–456（456 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6X3S Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with bicuculline methbromide Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 J94 (5S)-6,6-dimethyl-5-[(6R)-8-oxo-6,8-dihydrofuro[3,4-e][1,3]benzodioxol-6-yl]-5,6,7,8-tetrahydro[1,3]dioxolo[4,5-g]isoquinolin-6-ium × 2 MAN alpha-D-mannopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.12 Å
6X3T Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus propofol Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.55 Å
6X3U Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus flumazenil Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.50 Å
6X3V Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus etomidate Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.50 Å
6X3W Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus phenobarbital Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 UQA 5-ethyl-5-phenylpyrimidine-2,4,6(1H,3H,5H)-trione × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.30 Å
6X3X Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus diazepam Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.92 Å
6X3Z Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.23 Å
6X40 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus picrotoxin Deposited 2020-05-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.86 Å
7PBD a1b3 GABA-A receptor + GABA Deposited 2021-08-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 HSM HISTAMINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
7PBZ a1b3 GABA-A receptor + GABA + Zn2+ Deposited 2021-08-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 UND UNDECANE × 2 OCT N-OCTANE × 2 HEX HEXANE × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ZN ZINC ION × 1 HSM HISTAMINE × 1 D10 DECANE × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.79 Å
7PC0 GABA-A receptor bound by a-Cobratoxin Deposited 2021-08-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 HSM HISTAMINE × 1 ZN ZINC ION × 1 HEX HEXANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7T0W Complex of GABA-A synaptic receptor with autoimmune antibody Fab115 Deposited 2021-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–445（28 aa）
Chain D 28–339（312 aa）
Chain D 418–445（28 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7T0Z Complex of GABA-A synaptic receptor with autoimmune antibody Fab175 Deposited 2021-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–339（312 aa）
Chain B 418–445（28 aa）
Chain D 28–339（312 aa）
Chain D 418–445（28 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 MAN alpha-D-mannopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8DD2 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus Zolpidem Deposited 2022-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain D 28–339（312 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R5R Zolpidem × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.90 Å
8DD3 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus DMCM Deposited 2022-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain D 28–339（312 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R63 methyl 4-ethyl-6,7-dimethoxy-9H-pyrido[3,4-b]indole-3-carboxylate × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.90 Å
8PET Cryo-EM structure of the full-length human alpha1beta3 GABA(A) receptor (babba arrangement) in complex with nanobody Nb25, expressed with a 5-fold excess of beta3 over alpha1 Deposited 2023-06-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 37–456（420 aa）
Chain C 37–456（420 aa）
Not recorded R16 HEXADECANE × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 12 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
8SGO Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus pregnenolone sulfate Deposited 2023-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PTY PHOSPHATIDYLETHANOLAMINE × 6 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 1 A8W Pregnenolone sulfate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
8SI9 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus allopregnanolone Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 Y4B allopregnanolone × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.98 Å
8SID Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus dehydroepiandrosterone sulfate Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ZWY 17-oxoandrost-5-en-3beta-yl hydrogen sulfate × 1 PTY PHOSPHATIDYLETHANOLAMINE × 6 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 1 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.71 Å
8VQY Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus methaqualone Deposited 2024-01-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain D 28–339（312 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 A1ADG 2-methyl-3-(2-methylphenyl)quinazolin-4(3H)-one × 3 PTY PHOSPHATIDYLETHANOLAMINE × 4 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
8VRN Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus PPTQ Deposited 2024-01-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain D 28–339（312 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 A1ADN 3-(4-methylphenyl)-2-phenylquinazolin-4(3H)-one × 2 PTY PHOSPHATIDYLETHANOLAMINE × 5 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.57 Å
9CRS Native human GABAA receptor of beta2-alpha1-beta2-alpha1-gamma2 assembly Deposited 2024-07-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5s blot
Resolution 2.90 Å
9CRV Native human GABAA receptor of beta2-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5s blot
Resolution 3.18 Å
9CSB Native human GABAA receptor of beta3-alpha1-beta2-alpha2-gamma2 assembly Deposited 2024-07-23 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.34 Å
9CTJ Native human GABAA receptor of beta2-alpha1-beta3-alpha2-gamma2 assembly Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.74 Å
9CTP Native human GABAA receptor of beta2-alpha1-beta2-alpha3-gamma2 assembly Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.62 Å
9CTV Native human GABAA receptor of beta2-alpha1-gamma2-beta1-alpha2 assembly Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.36 Å
9CX7 Native human GABAA receptor of beta3-alpha1-gamma2-beta3-alpha2 assembly Deposited 2024-07-30 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.30 Å
9CXA Native human GABAA receptor of beta2-alpha1-beta3-alpha1-gamma2 assembly Deposited 2024-07-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 1–456（456 aa）
Chain D 1–456（456 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
9CXB Native human GABAA receptor of beta2-alpha1-beta1-alpha2-gamma2 assembly Deposited 2024-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.33 Å
9CXC Native human GABAA receptor of beta3-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-31 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.30 Å
9CXD Native human GABAA receptor of beta2-alpha1-beta1-beta1-gamma2 assembly Deposited 2024-07-31 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 28–456（429 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.36 Å
9DRX Human GABAA receptor of beta2-alpha1-beta2-alpha1-gamma2 subtype in complex with GABA plus Lamotrigine Deposited 2024-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain B 28–339（312 aa）
Chain B 418–456（39 aa）
Chain D 28–339（312 aa）
Chain D 418–456（39 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 IYJ (6M)-6-(2,3-dichlorophenyl)-1,2,4-triazine-3,5-diamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 2.95 Å
9EQG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with GABA and puerarin Deposited 2024-03-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 D10 DECANE × 11 HEX HEXANE × 10 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 2 R16 HEXADECANE × 5 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 A1H6W Puerarin × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 100 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
9FAJ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38, in a desensitised state (StateD1) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 37–449（413 aa）
Chain D 37–449（413 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 12 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 4 R16 HEXADECANE × 4 HEX HEXANE × 7 PLM PALMITIC ACID × 5 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9FAK CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD2) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain A 37–449（413 aa）
Chain D 37–449（413 aa）
Not recorded HEX HEXANE × 6 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 D10 DECANE × 7 PLM PALMITIC ACID × 8 CL CHLORIDE ION × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9FAM CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD3) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain A 37–449（413 aa）
Chain D 37–449（413 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 3 HEX HEXANE × 7 D10 DECANE × 1 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9FAP CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC1) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain A 39–443（405 aa）
Chain D 39–443（405 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 PLM PALMITIC ACID × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9FAQ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC2) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain A 39–443（405 aa）
Chain D 39–443（405 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PLM PALMITIC ACID × 5 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FAS CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with pregnenolone sulfate Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 37–445（409 aa）
Chain D 37–445（409 aa）
Not recorded HEX HEXANE × 8 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 17 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 R16 HEXADECANE × 5 A8W Pregnenolone sulfate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
9FAT CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, Megabody38 and pregnenolone sulfate Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain A 37–449（413 aa）
Chain D 37–449（413 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 3 CL CHLORIDE ION × 2 R16 HEXADECANE × 1 HEX HEXANE × 3 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 A8W Pregnenolone sulfate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9FFL Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived symmetric bound-closed state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded D10 DECANE × 6 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9FFM Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with Mb25 in the resting state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded D10 DECANE × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9FFN Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed 1 state of branch 1 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded D10 DECANE × 3 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FFO Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed 2 state of branch 1 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9FFP Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric open state of branch 1 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9FFQ Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric desensitised state of branch 1 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FFR Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed state of branch 2 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded CLR CHOLESTEROL × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FFS Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric open state of branch 2 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9FFT Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric desensitised state of branch 2 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FFU Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded D10 DECANE × 6 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
9FFV Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with Nb38 in the long-lived symmetric resting state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded D10 DECANE × 5 CL CHLORIDE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9FFW Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived symmetric bound-closed state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9FFX Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric bound-closed state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9FFY Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open1 state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FFZ Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 1 state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9FG0 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open 2 state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 37–339（303 aa）
Chain A 418–445（28 aa）
Chain D 37–339（303 aa）
Chain D 418–445（28 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9FG1 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 2 state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 D10 DECANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FG2 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9FG3 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 bound twice in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 32–339（308 aa）
Chain A 418–456（39 aa）
Chain D 32–339（308 aa）
Chain D 418–456（39 aa）
Not recorded D10 DECANE × 5 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FG4 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in the long-lived symmetric resting state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9FG5 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in complex with GABA in the short-lived symmetric bound-closed state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9FG6 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in complex with GABA and HSM in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 HSM HISTAMINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9FG7 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the short-lived symmetric bound-closed state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 3 CL CHLORIDE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9FG8 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded CL CHLORIDE ION × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FG9 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 2 R16 HEXADECANE × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9FGA Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to two PIP2 molecules and in complex with Mb38 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9FGB Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-A and in complex with Mb38 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9FGC Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-D and in complex with Mb38 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded D10 DECANE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9FGD Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs without PIP2 and in complex with Mb38 Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 D10 DECANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9FGF Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc in the long-lived symmetric resting state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 R16 HEXADECANE × 2 D10 DECANE × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FGG Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc bound to GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9FGH Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in large MSP2N2 nanodisc in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 28–456（429 aa）
Chain D 28–456（429 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9H9E Cryo-EM structure of the human GABAA receptor alpha1 subunit in complex with the assembly factor NACHO/TMEM35A Deposited 2024-10-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 37–456（420 aa）
Chain D 37–456（420 aa）
Not recorded PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 2 PC7 (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE × 2 PGT (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 2 CLR CHOLESTEROL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å

83 other PDB entries and 85 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name GBRA1_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–429； UniProt 28–456

Gamma-aminobutyric acid receptor subunit alpha-2

OrganismNot specified

UniProt P47869

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 7 其他Polymer 5 PDB declaration: heptameric（7） Consistent with protein copy count Chain D； UniProt 29–451 Not recorded Gamma-aminobutyric acid receptor subunit beta-2 × 2 （P47870） Gamma-aminobutyric acid receptor subunit alpha-1 × 1 （P14867） Gamma-aminobutyric acid receptor subunit gamma-2 × 1 （P18507） Kappa Fab_1F4 Light Chain × 1 IgG2b Fab_1F4 Heavy Chain × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE；3.5 s Blot. Resolution 3.19 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CT0 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9CRV Native human GABAA receptor of beta2-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 29–451（423 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5s blot
Resolution 3.18 Å
9CSB Native human GABAA receptor of beta3-alpha1-beta2-alpha2-gamma2 assembly Deposited 2024-07-23 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 29–451（423 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.34 Å
9CTJ Native human GABAA receptor of beta2-alpha1-beta3-alpha2-gamma2 assembly Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 29–451（423 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.74 Å
9CTV Native human GABAA receptor of beta2-alpha1-gamma2-beta1-alpha2 assembly Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 29–451（423 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.36 Å
9CX7 Native human GABAA receptor of beta3-alpha1-gamma2-beta3-alpha2 assembly Deposited 2024-07-30 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 29–451（423 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.30 Å
9CXB Native human GABAA receptor of beta2-alpha1-beta1-alpha2-gamma2 assembly Deposited 2024-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 29–451（423 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.33 Å
9CXC Native human GABAA receptor of beta3-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-31 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 29–451（423 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.30 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name GBRA2_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain D； PDBConstruct 1–423； UniProt 29–451

Gamma-aminobutyric acid receptor subunit gamma-2

OrganismNot specified

UniProt P18507

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Other combination Heteromer Protein × 7 其他Polymer 5 PDB declaration: heptameric（7） Consistent with protein copy count Chain E； UniProt 40–475 Not recorded Gamma-aminobutyric acid receptor subunit beta-2 × 2 （P47870） Gamma-aminobutyric acid receptor subunit alpha-1 × 1 （P14867） Gamma-aminobutyric acid receptor subunit alpha-2 × 1 （P47869） Kappa Fab_1F4 Light Chain × 1 IgG2b Fab_1F4 Heavy Chain × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE；3.5 s Blot. Resolution 3.19 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9CT0 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6D6U Human GABA-A receptor alpha1-beta2-gamma2 subtype in complex with GABA and flumazenil, conformation A Deposited 2018-04-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Chain E 439–467（29 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 Y01 CHOLESTEROL HEMISUCCINATE × 12 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 seconds blot time
Resolution 3.92 Å
6HUG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with picrotoxin and megabody Mb38. Deposited 2018-10-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6HUJ CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with picrotoxin, GABA and megabody Mb38. Deposited 2018-10-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
6HUK CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38. Deposited 2018-10-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded H0Z bicuculline methochloride × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.69 Å
6HUO CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with alprazolam (Xanax), GABA and megabody Mb38. Deposited 2018-10-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 08H 8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.26 Å
6HUP CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with diazepam (Valium), GABA and megabody Mb38. Deposited 2018-10-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.58 Å
6I53 Cryo-EM structure of the human synaptic alpha1-beta3-gamma2 GABAA receptor in complex with Megabody38 in a lipid nanodisc Deposited 2018-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6X3S Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with bicuculline methbromide Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 42–361（320 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 J94 (5S)-6,6-dimethyl-5-[(6R)-8-oxo-6,8-dihydrofuro[3,4-e][1,3]benzodioxol-6-yl]-5,6,7,8-tetrahydro[1,3]dioxolo[4,5-g]isoquinolin-6-ium × 2 MAN alpha-D-mannopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.12 Å
6X3T Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus propofol Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 42–361（320 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.55 Å
6X3U Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus flumazenil Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 42–361（320 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.50 Å
6X3V Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus etomidate Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 42–361（320 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.50 Å
6X3W Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus phenobarbital Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 42–361（320 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 UQA 5-ethyl-5-phenylpyrimidine-2,4,6(1H,3H,5H)-trione × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.30 Å
6X3X Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus diazepam Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 42–361（320 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.92 Å
6X3Z Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 42–361（320 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 3.23 Å
6X40 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus picrotoxin Deposited 2020-05-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 42–361（320 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.86 Å
7T0W Complex of GABA-A synaptic receptor with autoimmune antibody Fab115 Deposited 2021-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Chain E 440–467（28 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7T0Z Complex of GABA-A synaptic receptor with autoimmune antibody Fab175 Deposited 2021-11-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 40–361（322 aa）
Chain E 440–467（28 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 MAN alpha-D-mannopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8BHG GABA-A receptor a5 heteromer - a5V2 - Bretazenil Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 40–409（370 aa）
Not recorded QMU Bretazenil × 5 DMU DECYL-BETA-D-MALTOPYRANOSIDE × 5 NO3 NITRATE ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；279 K；22% PEG 400 0.37M potassium nitrate 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.5
Resolution 2.39 Å R-free 0.283
8DD2 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus Zolpidem Deposited 2022-06-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R5R Zolpidem × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.90 Å
8DD3 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus DMCM Deposited 2022-06-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R63 methyl 4-ethyl-6,7-dimethoxy-9H-pyrido[3,4-b]indole-3-carboxylate × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot
Resolution 2.90 Å
8SGO Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus pregnenolone sulfate Deposited 2023-04-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Chain E 439–467（29 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PTY PHOSPHATIDYLETHANOLAMINE × 6 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 1 A8W Pregnenolone sulfate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
8SI9 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus allopregnanolone Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Chain E 439–467（29 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 Y4B allopregnanolone × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.98 Å
8SID Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus dehydroepiandrosterone sulfate Deposited 2023-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Chain E 439–467（29 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ZWY 17-oxoandrost-5-en-3beta-yl hydrogen sulfate × 1 PTY PHOSPHATIDYLETHANOLAMINE × 6 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 1 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.71 Å
8VQY Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus methaqualone Deposited 2024-01-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 A1ADG 2-methyl-3-(2-methylphenyl)quinazolin-4(3H)-one × 3 PTY PHOSPHATIDYLETHANOLAMINE × 4 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
8VRN Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus PPTQ Deposited 2024-01-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 A1ADN 3-(4-methylphenyl)-2-phenylquinazolin-4(3H)-one × 2 PTY PHOSPHATIDYLETHANOLAMINE × 5 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.57 Å
9CRS Native human GABAA receptor of beta2-alpha1-beta2-alpha1-gamma2 assembly Deposited 2024-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–475（436 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5s blot
Resolution 2.90 Å
9CRV Native human GABAA receptor of beta2-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 40–475（436 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5s blot
Resolution 3.18 Å
9CSB Native human GABAA receptor of beta3-alpha1-beta2-alpha2-gamma2 assembly Deposited 2024-07-23 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 40–475（436 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.34 Å
9CTJ Native human GABAA receptor of beta2-alpha1-beta3-alpha2-gamma2 assembly Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 40–475（436 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.74 Å
9CTP Native human GABAA receptor of beta2-alpha1-beta2-alpha3-gamma2 assembly Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 40–475（436 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.62 Å
9CTV Native human GABAA receptor of beta2-alpha1-gamma2-beta1-alpha2 assembly Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 40–475（436 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.36 Å
9CX7 Native human GABAA receptor of beta3-alpha1-gamma2-beta3-alpha2 assembly Deposited 2024-07-30 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 40–475（436 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.30 Å
9CXA Native human GABAA receptor of beta2-alpha1-beta3-alpha1-gamma2 assembly Deposited 2024-07-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 1–475（475 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
9CXB Native human GABAA receptor of beta2-alpha1-beta1-alpha2-gamma2 assembly Deposited 2024-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 40–475（436 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.33 Å
9CXC Native human GABAA receptor of beta3-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-31 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 40–475（436 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.30 Å
9CXD Native human GABAA receptor of beta2-alpha1-beta1-beta1-gamma2 assembly Deposited 2024-07-31 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 40–475（436 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 3.36 Å
9DRX Human GABAA receptor of beta2-alpha1-beta2-alpha1-gamma2 subtype in complex with GABA plus Lamotrigine Deposited 2024-09-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 40–361（322 aa）
Chain E 448–475（28 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 IYJ (6M)-6-(2,3-dichlorophenyl)-1,2,4-triazine-3,5-diamine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；3.5 s Blot.
Resolution 2.95 Å
9EQG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with GABA and puerarin Deposited 2024-03-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–475（475 aa）
Not recorded PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 D10 DECANE × 11 HEX HEXANE × 10 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 2 R16 HEXADECANE × 5 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 A1H6W Puerarin × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 100 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
9FAJ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38, in a desensitised state (StateD1) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain C 64–467（404 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 12 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 4 R16 HEXADECANE × 4 HEX HEXANE × 7 PLM PALMITIC ACID × 5 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9FAK CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD2) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric
Chain C 64–467（404 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) HEX HEXANE × 6 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 D10 DECANE × 7 PLM PALMITIC ACID × 8 CL CHLORIDE ION × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9FAM CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD3) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain C 64–467（404 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 3 HEX HEXANE × 7 D10 DECANE × 1 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9FAN CryoEM structure of gamma2 subunit of GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 from desensitised state obtained by focused refinement Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 408–467（60 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 CL CHLORIDE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FAP CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC1) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain C 66–467（402 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 PLM PALMITIC ACID × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9FAQ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC2) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain C 66–467（402 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PLM PALMITIC ACID × 5 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FAR CryoEM structure of gamma2 subunit of GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 from a closed state, obtained by focused refinement Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 408–467（60 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 CL CHLORIDE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FAS CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with pregnenolone sulfate Deposited 2024-05-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 64–475（412 aa）
Not recorded HEX HEXANE × 8 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 17 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 R16 HEXADECANE × 5 A8W Pregnenolone sulfate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
9FAT CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, Megabody38 and pregnenolone sulfate Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric
Chain C 64–467（404 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 3 CL CHLORIDE ION × 2 R16 HEXADECANE × 1 HEX HEXANE × 3 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 A8W Pregnenolone sulfate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9FAU CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC1) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain C 65–467（403 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) HEX HEXANE × 6 D10 DECANE × 6 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 3 PLM PALMITIC ACID × 2 CLR CHOLESTEROL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FAV CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC2) Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain C 64–467（404 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) HEX HEXANE × 5 D10 DECANE × 4 R16 HEXADECANE × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9FAW CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, HEPES and Megabody25, in a desensitised state Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain C 60–467（408 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) HEX HEXANE × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CLR CHOLESTEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FAX CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with Megabody25, doubly occupied GARLH4 and Neuroligin2 TMD, in a closed state Deposited 2024-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric
Chain C 64–467（404 aa）
Chain E 64–467（404 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R16 HEXADECANE × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 CLR CHOLESTEROL × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 HEX HEXANE × 13 D10 DECANE × 5 PLM PALMITIC ACID × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FFV Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with Nb38 in the long-lived symmetric resting state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 40–361（322 aa）
Chain C 447–475（29 aa）
Not recorded D10 DECANE × 5 CL CHLORIDE ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9FFW Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived symmetric bound-closed state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 40–361（322 aa）
Chain C 447–475（29 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9FFX Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric bound-closed state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 40–361（322 aa）
Chain C 447–475（29 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9FFY Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open1 state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 40–361（322 aa）
Chain C 447–475（29 aa）
Not recorded D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FFZ Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 1 state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 40–361（322 aa）
Chain C 447–475（29 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9FG0 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open 2 state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 64–361（298 aa）
Chain C 447–475（29 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9FG1 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 2 state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 40–361（322 aa）
Chain C 447–475（29 aa）
Not recorded ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 D10 DECANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FG2 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 40–361（322 aa）
Chain C 447–475（29 aa）
Not recorded D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9FG3 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 bound twice in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 40–361（322 aa）
Chain C 447–475（29 aa）
Not recorded D10 DECANE × 5 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9FG7 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the short-lived symmetric bound-closed state Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–475（475 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 3 CL CHLORIDE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9FG8 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–475（475 aa）
Not recorded CL CHLORIDE ION × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FG9 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–475（475 aa）
Not recorded CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 2 R16 HEXADECANE × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9FGA Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to two PIP2 molecules and in complex with Mb38 Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9FGB Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-A and in complex with Mb38 Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9FGC Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-D and in complex with Mb38 Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded D10 DECANE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9FGD Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs without PIP2 and in complex with Mb38 Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 D10 DECANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9FGF Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc in the long-lived symmetric resting state Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–475（475 aa）
Not recorded CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 R16 HEXADECANE × 2 D10 DECANE × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9FGG Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc bound to GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9FGH Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in large MSP2N2 nanodisc in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–475（475 aa）
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å

70 other PDB entries and 70 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name GBRG2_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain E； PDBConstruct 1–436； UniProt 40–475

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9ct0

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9ct0
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9ct0
Deposition date deposition_date2024-07-24
Structure title titleNative human GABAA receptor of beta2-alpha1-beta2-alpha2-gamma2 assembly
Keywords keywordsIon channels, Heteropentamer, Receptor, Inhibitory., MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier40.55 Å
Radius of gyration Rg (electron density) rg_electron40.14 Å
Forward intensity I(0) i0598058000.00
Molecular weight molecular_weight209330.0 kDa
Excluded volume excluded_volume265550 ų
Envelope volume envelope_volume343640 ų
Hydration-shell volume shell_volume70400 ų
Envelope diameter envelope_diameter138.8 Å
Shell Rg shell_rg46.43 Å
Envelope Rg envelope_rg39.71 Å
Shape Rg shape_rg40.13 Å
Total Rg total_rg40.51 Å
Total atoms total_atoms14736
Residues n_residues1761
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax130.6 Å
Rg (real space) rg_real40.46 Å
Rg uncertainty (real space) rg_real_error1.02 Å
I(0) (real space) i0_real5.9810e+08
I(0) uncertainty (real space) i0_real_error1.1200e+07
Rg (reciprocal space) rg_reciprocal40.55 Å
I(0) (reciprocal space) i0_reciprocal598100000.0000
Solution quality estimate total_estimate0.8921
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary50.2 Å
Skewness Skewness skewness0.253
Kurtosis Kurtosis kurtosis-0.449
Angular range angular_range— – 0.1950 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha60060000.0000
Real-space data points n_real_points40
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.893; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.916
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (13)

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8. Citations (1)

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9. Files and Curves (10)