SAXSdb — 9hyf

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9hyf
沉积日期 deposition_date	2025-01-09
结构标题 title	Cryo-EM structure of the C. elegans UBR4/KCMF1 complex (composite map)
关键词 keywords	Ubiquitin ligase Protein quality control, LIGASE; LIGASE
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	— Å
回转半径 Rg (电子) rg_electron	100.30 Å
零角强度 I(0) i0	11394100000.00
分子量 molecular_weight	919190.0 kDa
排除体积 excluded_volume	1153800 Å³
包络体积 envelope_volume	2518500 Å³
水化壳体积 shell_volume	208800 Å³
包络直径 envelope_diameter	279.1 Å
壳层 Rg shell_rg	103.50 Å
包络 Rg envelope_rg	91.86 Å
形状 Rg shape_rg	100.30 Å
总 Rg total_rg	100.20 Å
总原子数 total_atoms	64398
残基数 n_residues	8016
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	240.1 Å
Rg (实空间) rg_real	97.62 Å
Rg 误差 (实空间) rg_real_error	0.49 Å
I(0) (实空间) i0_real	1.0920e+10
I(0) 误差 (实空间) i0_real_error	1.9700e+08
Rg (倒空间) rg_reciprocal	101.50 Å
I(0) (倒空间) i0_reciprocal	11430000000.0000
解质量估计 total_estimate	0.9030
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	144.1 Å
偏度 Skewness skewness	-0.100
峰度 Kurtosis kurtosis	-0.900
角度范围 angular_range	— – 0.0750 Å⁻¹
当前正则化参数 α current_alpha	1.0420
最高正则化参数 α highest_alpha	306100000.0000
实空间数据点数 n_real_points	16
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.997; Stabil: 0.938; Sysdev: 1.000; Positv: 1.000; Valcen: 0.938; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (3)

Entity 1 polymer

描述 description	UBR-type domain-containing protein
分子量 formula_weight	441954.7 kDa
来源方法 src_method	man
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	64398

MDPRETAIGLVRYVTENAAKDFDGESIAAIIYRLRVVENYVSSDVDIICEAMRILIKKLVNLPPITFSPDVWMPIMTSITEGAIEPQWRIMMESIDISKFSSTSSNFVHMDSITASSTKCSEQITSICKGFALEFGLTRRLFSAIWNTDLEETARKMMNQEWDSVKIDSSPDSAKQAAGLIEKSIFDLFSEMKDHETFANELYYAALARILNNSRRLQENKKPAIQDSEDISFYESSLHIYDELIKKLWPHLSKKDPNVKKNTLIIFEKLISLSPTDVLVQRITEKFIDSCEWLKISGEEICTSMEPRLWKIVGSLVSRILDLKKTIPYEIVSYAIAVHITQEVTLASPEVSDCGPDSFDSTCDQPSNMKERFLRHMVDSYFQTRSSLLSIIQFLRTAKSSEEIVQFMRVFYPELVFGNETLITHAAIEMLRESASHHSIKADDFISIVLSVIDAPAIVRIIDVISCIKDEKVRKEAVEKTIIRMANDLEDEDQKREGYVNHLHENGSFWNDIAKYADASILDKAGKRFVEVSVDSMDIRSSVLATDIFLTILKLKLTSEAEREPESCVICNVSYSNIKIDLEKWNKMGNILVECNMVKRSKSEALPLFTIHQLENIDLPMRDLVYVVTQMKFLDTKEGMELHRKFYDGLPGVNVIFEKFMSKSHKIELCMKLLDEFAKIIAINEKSLIFSLDHENILDWLEEIGVLDSPQITEKLVDICFSIEVWDVLTLLQLDGPECHYWDLQMFGRFWKTSLMDLLDEKMMKKVNEKMGSILKEQYDKQSHVAKATREKRSNGKFPNRPKVADWEEQLLLMHNRIAGHLTKKKVEDFADESTQKFTWLLGVCSGQMSYKKEVAVDAEKILSRLYPDAEKRNEVLYHFGVSSILKGLDRPRGKLSLHILFMQIYLDLVQIDSKSWKIDDSVQKLSRRLGGFNEWLMIVDEEKREDDGGFRIYIVLNLSHYFWELLEGCKASQVVDAHAILKIRKFAEVLASILDKITFWPNPKLHAYYYIAQFLEPLETIFHFPEIAEQNRKVIESFFKQLFDKLLEQKYQEGLLQDTKLIIQKTDKYLSSSLNLFNEYNTQEPSKIYPVNEIFSLFCRYGSENVHLYCLKMIKKSLQTLASNILEHEHILKGEVCIETELQKRLVCDAVLLTEFFGYFSCIYAQVSENQPSEHDDVAKAFMLLDSDIHLKTKSRKRSTQNSVAVGSHRHQTAENMDLNFCTYKSTGRAYVTQHWYNCYTCNMMESEGVCSVCAINCHRGHDLAYSKKGSFFCDCGEKKCGAMQGIYHLPNSMYSLRGQLPEKNGDKTLPKIRNVFEHRFENLNSNCCDELKSALNDVQEEFKEVQDDLEQILEAVDFANQKALQVTEERLAVLESFNDMDDVIISEKEKFIEPLESGHFLPTRRDDLPVQELRVASSQHKIRELSDIIKLDDGTELLVLIPESIQTCLQLHYMDTRTNLIQGMHALRTETEQIPFNARSLHVSGNRLVVCGQYEFFALRFSPQGDVIDRAHIKLLENGASSSMNNNPVVRAKFCREIESDKRRRQLIAVATMQYIRIYDLTLHETNFVEEMVLPAGNVEDVEIINQEDGNVRILVLSSSGYLYEHNISVFNAENNSIFLTNVVNTPGMDMNGDGVSLHYSSTFNLLFVSLENGAFVAQLPEPTGNSTAPIYDWKHLNIKNPVDAWKETSGIIACLSTNCNHQVNYFHPTVGKILLQKTSVKRSIMTYFLMTSAKNQSVYSVLIYPNVPTCEIWETSWNNVHDLWIDDVPTERYAVEVVEQKSTQIPIDRDELVLLAEQCEQIRTVDWNCREIGMFYTHEELNNRLSSSDSLPITLIQQTHFKLSARITNFRQIVRMIRVEVEGNTGPEYLKIGTTRYSISSRGPKTFDLRLSREESLALDHRDITIEVIPRSNQNRVTLKSLRLLGCDRSAMDEIQPRYERQPILTNSNKLVYSILEFATLSGLEWAGNMAKKHLSRKLNHPAVCSVSTRAIVKCHPSVDEELFKIIDGAYLQEWKALIDWTESEGFGEMRLHHVEQLLDRMEAVRTRWPYFVKSLKREFGTVTSFVELMRNEMKRMPLHRCQMMAQAIVKIVFGLLSNGTNEAEQLIHVFLNIFTDQDTYHLANDMRSAVQETISRFENALKEEKKLMVEHENMDKESVLRIKNYGFSPFYGAPRIIAKTPESMLIAKIAETIPIDSEENFKWLEQLISMILEKLTRSNSTVTWQNLSDSPSYNLSRVLASCLAICDPVIIRNHFSRLIHIIKYDVEKIFPMSEKSYSNYSLLRSVELLLFVCLEKRGDESKENQEMLDSIVHDLQAVGIRNLCLKILEKVIPHWKDRGPSTFSRNSAESRKVWLPHVPLVSSSANPPNTSIMNWPSTSEDSYIIACTDLILLIPQHLQELDRRKSVPRDDQWIQKLCQLASLSSGCSAYRQCKKLLLAMCHNDENKYKIMRDKYKMQDLLQQLVKKYLDVSKEVGGHQQLTEIVDVLASITKLALIRPDMWRDVCSTHTTWLLRLACYTTDVVASQVVELLIVAVRDSSVGGQLSIQLADSIVEAENGEFIEKIIKRFLIGRDEQLRWTLHGMLRSVIQLASRQNQCILVKKLYNTIYPLAANLGVQGAQLVDLIATYAPRVFSSTELVAMTQSEISTIEKIRNTLNGDGYQGMYKLMSDLGLGWKSINFDRNPCLVCFTSKGSHDVVKMTSIKSDIRYSANTIIYKLISNYEVSKVTIKLTELKKSKSIKKVSLYYSAKSVESVDLKLHPELWRKCAMVTVAENETVINLSLAVPVVTSSLIVEFEEVVDHRGSSQLHCPRCTVPVRTHSGVCESCGENAFQCAKCRAINYVEKEPFLCQSCGFCKYAQISLFVVCRALPGAQHITCDAERGQCVQEITQLLIKMETTKTKLTGYRAACESLYLRNRPLPPYKLHVENNHTSEFFDANGTMNIEQLPFQSITMPMNNLAVAIRTLHSELCQQTQQLMYLHEELNRYDHASEVSVVFHKPQNISYYSTGQSCFGCLCNQLLHSVALLHSSCDDENALNLILSSDSLIEKLGVLAQTYETLREEVEELLVRLMFDRLDVTTKVEKLIHTGDINLSVMVKSLMYAADPTWQQKLKLLIRLAMDKRDENCCLQALMVLSKYLEATKSVTLDERKKIKLTPNKNITKWLTTDEEWKDCFSVASTSTAPTKSLPSNPYQWITDCLFSQWMSVRSAANQLLVNLSRQQFHEPVALLILCENMSKLSDVPSSVCDQFMCSAHTIIDSSVNTKARLFVQQFHVYLIKRIHEECAKLHEQSVNLLSDNMFGERLRCFVELLSLLLSGSNVENVLLKAGADDLLIFLLHSTIFLKRIMTRRTRATDSSRIALEKLLKRVSCRDGTKLMSVCVESLKLVKDTSTLGIIVGVMMEIMDPQQETEESFLIQIEKDIAQEDFLQGRMTHNPYNSSDPGMGPLMRDIKNKICRDTEMIALMEDDNGMELLVNGNIISLSLSVRDVYDRLWKRSNNGSPMLIVYRMRGLMGDAVETFIENFGVADNSEADDEEDEQLVRMTHCLMECGGIDKLMDLLTTNVDSSSGRFLLCYLRKIFERIVKIPIGRKILVQRRMVERMMSVIRTCCADPTNESKVLIGMELYKVVEFVVSDKHVQDILGGIREDDAMWWFDLFEKRGNEEGSVTELHRKTAQILDQMTMSIGNIVLGNDASEGVLVKMYEKVLKWEQIDSGAPPSGATDHQNRSRITKKDQIMLMTEQLATITSNIISSAHGIRLKQKILDSGVISSTCNYLTKDLPNLYQPTESPEWKVFLARPSLKLILTLLAGLARGHQASQKEIAKTTLKLMHRLEQVASDNSIGTLAENVIEALNE

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Trichoplusia ni

Entity 2 polymer

描述 description	E3 ubiquitin-protein ligase kcmf-1
分子量 formula_weight	57890.0 kDa
EC 编号 ec_number	2.3.2.27
来源方法 src_method	man
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C,D

MVTPLTGTHEGVSCDGCAFTAFAGNRYKCLRCSDYDLCFSCFTTKNYGDQQTIADIPIHDESHPMQLILSSVDFDLVYQGDPTRHYDERKIVSFTCPYCNITGLTERQFGTHVLSQHPEAPGYSVICPLCIGNTEMEHIQSKETENLSVHWTEIHLHTMENLFRSTEPITTRPVQRRPMLARRGNRAGVSRTAAQGRPLQDEIGAEMAALLRNMGPDSVEDIRRFTEMMTVPLLPGIRSSQRLMTSTGDRPTVVVESAVTHQDPNVQQVIRPLATIPIYPPTSDESGDETPQPAADSADESEDDNDIQELDDMQPIVEDEALKKDEFWKTLKTRISEEDVDLILETMKSTAKVKEDIDDKMPVWTQRPLKRLANAAQVTTTSDSEGDPGWLPLSFETTPIRSTGCGGYWSDKRFLRPRKMQREQSVASSNAEIMEKAEIALALIRASCLHEPVFTDPTKPDIALKEALQHLRLGEKPAKMMEYQAAEELVNMPERDPITTGEMEVEIPDFTARGY

来源 Source

来源生物 organism	Caenorhabditis elegans
NCBI 分类编号 ncbi_taxonomy_id	6239
宿主生物 host_organism	Trichoplusia ni

Entity 3 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	18

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.000 Å
最低分辨率 resolution_low	— Å
R 因子 r_factor	—
R_work r_work	—
R_free r_free	—

分辨率 resolution	3.00 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	691759

fit	/app/data/saxs_computation/hy/9hyf/9hyf.fit
pddf	/app/data/pr_computation/hy/9hyf/9hyf.pr.out
profile	/app/data/saxs_computation/hy/9hyf/9hyf.dat

Cryo-EM structure of the C. elegans UBR4/KCMF1 complex (composite map)

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

冷冻电镜 EM 3D

5. 实体与聚合体信息 (3)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 non-polymer

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)