Macrophage migration inhibitory factor
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein homooligomer Homooligomer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count | Chain A； UniProt 1–115 Chain B； UniProt 1–115 Chain C； UniProt 1–115 | Mutation：S61H/Y100H | ZN ZINC ION × 3 CO3 CARBONATE ION × 1 SO4 SULFATE ION × 5 GOL GLYCEROL × 4 IPA ISOPROPYL ALCOHOL × 3 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol | Resolution 1.20 Å R-free 0.174 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 9JIY | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1CA7 MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH HYDROXPHENYLPYRUVATE Deposited 1999-02-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | EN1 (2E)-2-hydroxy-3-(4-hydroxyphenyl)prop-2-enoic acid × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.50 Å R-free 0.263 |
| 1CGQ MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH ALANINE INSERTED BETWEEN PRO-1 AND MET-2 Deposited 1999-03-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：INS(A-M2) Mutation：INS(A-M2) Mutation：INS(A-M2) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.00 Å R-free 0.275 |
| 1GCZ MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) COMPLEXED WITH INHIBITOR. Deposited 2000-08-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 10 CIT CITRIC ACID × 3 YZ9 7-HYDROXY-2-OXO-CHROMENE-3-CARBOXYLIC ACID ETHYL ESTER × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；ammonium sulfate, sodium citrate, pH 5.0, vapor diffusion/hanging drop, temperature 298.0K
|
Resolution 1.90 Å R-free 0.240 |
| 1GD0 HUMAN MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) Deposited 2000-08-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 7 CIT CITRIC ACID × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；ammonium sulfate, sodium citrate, pH 5.0, vapor diffusion : hanging drop, temperature 298.0K
|
Resolution 1.50 Å R-free 0.214 |
| 1GIF HUMAN GLYCOSYLATION-INHIBITING FACTOR Deposited 1996-02-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1LJT Crystal Structure of Macrophage Migration Inhibitory Factor complexed with (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole-acetic acid methyl ester (ISO-1) Deposited 2002-04-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | HDI 3-(4-HYDROXYPHENYL)-4,5-DIHYDRO-5-ISOXAZOLE-ACETIC ACID METHYL ESTER × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；ammonium sulfate, isopropanol, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.262 |
| 1MIF MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) Deposited 1996-01-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1P1G MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH PRO-1 MUTATED TO GLY-1 Deposited 1999-03-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：P1G Mutation：P1G Mutation：P1G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.50 Å R-free 0.270 |
| 2OOH Crystal Structure of MIF bound to a Novel Inhibitor, OXIM-11 Deposited 2007-01-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | SO4 SULFATE ION × 8 OX3 4-HYDROXYBENZALDEHYDE O-(CYCLOHEXYLCARBONYL)OXIME × 2 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；296 K；50% saturated ammonium sulfate, 4% isopropanol, 0.1 M tris(hydroxymethyl)aminomethane,mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.85 Å R-free 0.218 |
| 2OOW MIF Bound to a Fluorinated OXIM Derivative Deposited 2007-01-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | SO4 SULFATE ION × 5 OX4 3-FLUORO-4-HYDROXYBENZALDEHYDE O-(CYCLOHEXYLCARBONYL)OXIME × 2 GOL GLYCEROL × 6 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；296 K；50% saturated ammonium sulfate, 4% isopropanol, 0.1
M tris(hydroxymethyl)aminomethan; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.75 Å R-free 0.204 |
| 2OOZ Macrophage Migration Inhibitory Factor (MIF) Complexed with OXIM6 (an OXIM Derivative Not Containing a Ring in its R-group) Deposited 2007-01-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | SO4 SULFATE ION × 5 OX5 4-HYDROXYBENZALDEHYDE O-(3,3-DIMETHYLBUTANOYL)OXIME × 2 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；296 K；50% saturated ammonium sulfate, 4% isopropanol, 0.1
M tris(hydroxymethyl)aminomethan; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.80 Å R-free 0.225 |
| 3B9S Macrophage Migration Inhibitory Factor (MIF) complexed with Inhibitor, 4-IPP. Deposited 2007-11-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | GOL GLYCEROL × 3 RW1 4-phenylpyrimidine × 3 SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2M ammonium sulfate, 4% isopropanol, 0.1 M tris(hydroxymethyl)aminomethan; mixed with protein:inhibitor complex in a 1:10 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.230 |
| 3CE4 Structure of Macrophage Migration Inhibitory Factor Covalently Inhibited by PMSF Treatment Deposited 2008-02-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 5 PMF PHENYLMETHYLSULFONYL FLUORIDE × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；293 K；50% saturated ammonium sulfate, 4% isopropanol, 0.1M tris(hydroxymethyl)aminomethane; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.209 |
| 3DJH Macrophage Migration Inhibitory Factor (MIF) at 1.25 A Resolution Deposited 2008-06-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 GOL GLYCEROL × 4 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；50% Saturated ammonium sulfate, 3% Isopropanol, 0.1 M Tris(hydroxymethyl)aminomethane, mixed with protein in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.25 Å R-free 0.186 |
| 3DJI Crystal Structure of Macrophage Migration Inhibitory Factor Bound to an Acetaminophen Dimer Derived from NAPQI Deposited 2008-06-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 9 3E1 N,N'-(6,6'-dihydroxybiphenyl-3,3'-diyl)diacetamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；2 M Ammonium sulfate, 3% Isopropanol, 0.1 M Tris(hydroxymethyl)aminomethane, mixed with protein-inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.95 Å R-free 0.247 |
| 3DJI Crystal Structure of Macrophage Migration Inhibitory Factor Bound to an Acetaminophen Dimer Derived from NAPQI Deposited 2008-06-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；2 M Ammonium sulfate, 3% Isopropanol, 0.1 M Tris(hydroxymethyl)aminomethane, mixed with protein-inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.95 Å R-free 0.247 |
| 3HOF Structure of macrophage migration inhibitory factor (MIF) with caffeic acid at 1.9A resolution Deposited 2009-06-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Not recorded | DHC CAFFEIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；296 K；30-42.5% MPD_P1K_P3350 mix (MDL Morpheus screen), 0.1M buffer 1 mix (MDL morpheus screen), pH 5.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.90 Å R-free 0.200 |
| 3IJG Macrophage Migration Inhibitory Factor (MIF) Bound to the (R)-Stereoisomer of AV1013 Deposited 2009-08-04 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 10 AVR (2R)-2-amino-1-[2-(1-methylethyl)pyrazolo[1,5-a]pyridin-3-yl]propan-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；310 K；2 M ammonium sulfate, 3% isopropanol, 0.5 M NaCl, 0.1 M tris(hydroxymethyl)aminomethane; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
|
Resolution 1.70 Å R-free 0.206 |
| 3IJJ Ternary Complex of Macrophage Migration Inhibitory Factor (MIF) Bound Both to 4-hydroxyphenylpyruvate and to the Allosteric Inhibitor AV1013 (R-stereoisomer) Deposited 2009-08-04 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | EN1 (2E)-2-hydroxy-3-(4-hydroxyphenyl)prop-2-enoic acid × 3 ENO 3-(4-HYDROXY-PHENYL)PYRUVIC ACID × 3 CL CHLORIDE ION × 8 AVR (2R)-2-amino-1-[2-(1-methylethyl)pyrazolo[1,5-a]pyridin-3-yl]propan-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；310 K；2 M ammonium sulfate, 3% isopropanol, 0.5 M NaCl, 0.1 M tris(hydroxymethyl)aminomethane; mixed in a 1:1 ratio with the protein:substrate:inhibitor complex, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
|
Resolution 1.25 Å R-free 0.185 |
| 3JSF Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 638 at 1.93a resolution Deposited 2009-09-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | XV1 7-(2-fluorobenzyl)quinolin-8-ol × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M AMMONIUM SULFATE, 2 mM EDTA, 0.1 M HEPES, PH 6.5, VAPOR DIFFUSION/HANGING DROP, TEMPERATURE 298.0K , VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.93 Å R-free 0.218 |
| 3JSG Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 707 at 1.58a resolution Deposited 2009-09-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 0IN 7-(pyridin-3-ylmethyl)quinolin-8-ol × 3 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M AMMONIUM SULFATE, 2 mM EDTA, 0.1 M HEPES, PH 6.5, VAPOR DIFFUSION/HANGING DROP, TEMPERATURE 298.0K , VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.58 Å R-free 0.201 |
| 3JTU Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 708 at 1.86a resolution Deposited 2009-09-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | ZIN 7-(pyridin-2-ylmethyl)quinolin-8-ol × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M AMMONIUM SULFATE, 2 mM EDTA, 0.1 M HEPES, PH 6.5, VAPOR DIFFUSION/HANGING DROP, , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.86 Å R-free 0.192 |
| 3L5P Crystal structure of macrophage migration inhibitory factor (MIF) with imidazopyridazinol inhibitor at 1.80A resolution Deposited 2009-12-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 428 2-(1-methylethyl)imidazo[1,2-b]pyridazin-6-ol × 1 GOL GLYCEROL × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.221 |
| 3L5R Crystal structure of macrophage migration inhibitory factor (MIF) with phenylchromenone inhibitor at 1.94A resolution Deposited 2009-12-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 47X 3-(3,4-dihydroxyphenyl)-7-hydroxy-4H-chromen-4-one × 1 SO4 SULFATE ION × 5 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.94 Å R-free 0.233 |
| 3L5S Crystal structure of macrophage migration inhibitory factor (MIF) with imidazopyrimidinylphenyl inhibitor at 1.86A resolution Deposited 2009-12-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 88X 5-ethyl-2-(phenylcarbonyl)imidazo[1,2-a]pyrimidin-7(1H)-one × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.86 Å R-free 0.201 |
| 3L5T Crystal structure of macrophage migration inhibitory factor (MIF) with thiophenepiperazinylquinolinone inhibitor at 1.86A resolution Deposited 2009-12-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 956 1-methyl-2-oxo-4-[4-(thiophen-2-ylcarbonyl)piperazin-1-yl]-1,2-dihydroquinoline-3-carbonitrile × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.86 Å R-free 0.214 |
| 3L5U Crystal structure of macrophage migration inhibitory factor (MIF) with benzothiazole inhibitor at 1.90A resolution Deposited 2009-12-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | ZEC 6-HYDROXY-1,3-BENZOTHIAZOLE-2-SULFONAMIDE × 3 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.209 |
| 3L5V Crystal structure of macrophage migration inhibitory factor (MIF) with glycerol at 1.70A resolution Deposited 2009-12-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | GOL GLYCEROL × 3 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.211 |
| 3SMB Phenethylisothiocyanate Covalently Bound to Macrophage Migration Inhibitory Factor (MIF) Deposited 2011-06-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | LE2 N-(2-phenylethyl)thioformamide × 3 CL CHLORIDE ION × 9 NA SODIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% isopropanol, 0.1
M tris(hydroxymethyl)aminomethane; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.197 |
| 3SMC Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane Deposited 2011-06-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | LE3 N-{4-[(R)-methylsulfinyl]butyl}thioformamide × 3 CL CHLORIDE ION × 7 NA SODIUM ION × 4 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% isopropanol, 0.1
M tris(hydroxymethyl)aminomethane; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.186 |
| 3U18 Chicago Sky Blue 6B, A Novel Inhibitor for Macrophage Migration Inhibitory Factor Deposited 2011-09-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | Y0X 6,6'-[(3,3'-dimethoxybiphenyl-4,4'-diyl)di(E)diazene-2,1-diyl]bis(4-amino-5-hydroxynaphthalene-1,3-disulfonic acid) × 1 IPA ISOPROPYL ALCOHOL × 4 GOL GLYCEROL × 9 EDO 1,2-ETHANEDIOL × 3 NA SODIUM ION × 4 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8；310 K；100 mM p425 dissolved in 2.0 M ammonium sulfate, 4% 2-propanol, 0.1 M Tris, VAPOR DIFFUSION, temperature 310K, pH 8
|
Resolution 1.90 Å R-free 0.177 |
| 3WNR Multiple binding modes of benzyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2013-12-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 9BE N-benzylthioformamide × 3 SO4 SULFATE ION × 5 GOL GLYCEROL × 5 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9M ammonium sulfate, 100mM Tris pH 8.0, 200mM NaCl, 4%(v/v) 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.01 Å R-free 0.200 |
| 3WNS Allyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2013-12-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 9AI N-prop-2-en-1-ylthioformamide × 3 SO4 SULFATE ION × 6 IPA ISOPROPYL ALCOHOL × 1 CL CHLORIDE ION × 1 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9M ammonium sulfate, 100mM Tris pH 8.0, 200mM NaCl, 4%(v/v) 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.66 Å R-free 0.258 |
| 3WNT Multiple binding modes of benzyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2013-12-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 9BE N-benzylthioformamide × 3 SO4 SULFATE ION × 5 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9M ammonium sulfate, 100mM Tris pH 8.0, 200mM NaCl, 4%(v/v) 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.07 Å R-free 0.263 |
| 4ETG Crystal Structure of MIF L46G mutant Deposited 2012-04-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：L46G Mutation：L46G Mutation：L46G | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；291 K；1.8M Ammonium sulfate in 0.1M Tris and 3% isopropanol, pH 7.5, VAPOR DIFFUSION, temperature 291K
|
Resolution 1.61 Å R-free 0.249 |
| 4EUI Crystal Structure of MIF L46F mutant Deposited 2012-04-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：L46F Mutation：L46F Mutation：L46F | SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；291 K；1.8M Ammonium sulfate in 0.1M Tris and 3% isopropanol, pH 7.5, VAPOR DIFFUSION, temperature 291K
|
Resolution 1.70 Å R-free 0.259 |
| 4EVG Crystal Structure of MIF L46A mutant Deposited 2012-04-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：L46A Mutation：L46A Mutation：L46A | SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；291 K；1.8M Ammonium sulfate in 0.1M Tris (pH 7.5) and 3% isopropanol, VAPOR DIFFUSION, temperature 291K
|
Resolution 1.70 Å R-free 0.214 |
| 4F2K Macrophage Migration Inhibitory Factor covalently complexed with phenethylisothiocyanate Deposited 2012-05-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | LE2 N-(2-phenylethyl)thioformamide × 3 SO4 SULFATE ION × 4 IPA ISOPROPYL ALCOHOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；1.9M (NH4)2SO4, 100mM Tris pH 8.0, 200mM NaCl, 4% v/v isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.53 Å R-free 0.228 |
| 4GRN crystal structure of PAAM mutant of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 12 SO4 SULFATE ION × 4 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2M NH42SO4, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.25 Å R-free 0.141 |
| 4GRO Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.230 |
| 4GRO Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
Chain D
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.230 |
| 4GRO Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
Chain H
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.230 |
| 4GRO Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain G
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.230 |
| 4GRP Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | SO4 SULFATE ION × 5 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.6；293 K；1.6M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.27 Å R-free 0.169 |
| 4GRQ Characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 6 SO4 SULFATE ION × 1 AVL 2-methyl-1-[2-(propan-2-yl)pyrazolo[1,5-a]pyridin-3-yl]propan-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；310 K；1.6M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
|
Resolution 1.65 Å R-free 0.161 |
| 4GRR characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
3–115（113 aa）
Chain B
3–115（113 aa）
Chain C
3–115（113 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | SO4 SULFATE ION × 5 CL CHLORIDE ION × 11 AVR (2R)-2-amino-1-[2-(1-methylethyl)pyrazolo[1,5-a]pyridin-3-yl]propan-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；310 K；1.6M ammmonium sualfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
|
Resolution 1.47 Å R-free 0.172 |
| 4GRU crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 12 DIO 1,4-DIETHYLENE DIOXIDE × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0M ammonium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.92 Å R-free 0.230 |
| 4GUM Cystal structure of locked-trimer of human MIF Deposited 2012-08-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N110C Mutation：N110C Mutation：N110C | CL CHLORIDE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；0.2M LiSO4, 3% DMSO, pH8.0, 33% PEG4000 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.33 Å R-free 0.272 |
| 4GUM Cystal structure of locked-trimer of human MIF Deposited 2012-08-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Mutation：N110C Mutation：N110C Mutation：N110C | CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；0.2M LiSO4, 3% DMSO, pH8.0, 33% PEG4000 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.33 Å R-free 0.272 |
| 4GUM Cystal structure of locked-trimer of human MIF Deposited 2012-08-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain G
2–115（114 aa）
Chain H
2–115（114 aa）
Chain I
2–115（114 aa）
|
Mutation：N110C Mutation：N110C Mutation：N110C | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；0.2M LiSO4, 3% DMSO, pH8.0, 33% PEG4000 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.33 Å R-free 0.272 |
| 4K9G 1.55 A Crystal Structure of Macrophage Migration Inhibitory Factor bound to ISO-66 and a related compound Deposited 2013-04-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 1Q2 (4R,6Z)-6-(3-fluoro-4-hydroxyphenyl)-4-hydroxy-6-iminohexan-2-one × 3 1Q1 1-[(5S)-3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydro-1,2-oxazol-5-yl]propan-2-one × 1 CL CHLORIDE ION × 6 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；293 K；1.1 mM (14 mg/ml) MIF , 10 mM inhibitor in 10% DMSO, 18 mM NaCl, 18 mM tris(hydroxymethyl)aminomethane mixed 1:1 with reservoir containing 2 M ammonium sulfate, 0.1 M tris(hydroxymethyl)aminomethane, 3% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.220 |
| 4OSF 4-(2-isothiocyanatoethyl)phenol inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2014-02-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 4MT N-[2-(4-hydroxyphenyl)ethyl]thioformamide × 3 SO4 SULFATE ION × 12 CL CHLORIDE ION × 2 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9M ammonium sulfate, 100mM Tris pH 8.0, 200mM NaCl, 4%(v/v) 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.62 Å R-free 0.223 |
| 4OYQ (6-isothiocyanatohexyl)benzene inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2014-02-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 1X2 6-isothiocyanatohexylbenzene × 3 SO4 SULFATE ION × 4 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9 M ammonium sulfate, 100 mM Tris pH 8.0, 200 mM NaCl, 4%(v/v) 2-propanol
|
Resolution 1.70 Å R-free 0.195 |
| 4P01 Crystal Structure Analysis of Macrophage Migration Inhibitory Factor in complex with N-[(4-cyanophenyl)methyl]methanethioamide Deposited 2014-02-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 1ZW N-[(4-cyanophenyl)methyl]methanethioamide × 2 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 1 CL CHLORIDE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 2.07 Å R-free 0.228 |
| 4P0H Crystal Structure Analysis of Macrophage Migration Inhibitory Factor in complex with Dimethylformamide Deposited 2014-02-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | DMF DIMETHYLFORMAMIDE × 3 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5
|
Resolution 1.93 Å R-free 0.217 |
| 4PKK Crystal structure of Macrophage Migration inhibitory factor in complex with furan-2-ylmethyl)imino methanethiol Deposited 2014-05-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 31E (Z)-[(furan-2-ylmethyl)imino]methanethiol × 3 GOL GLYCEROL × 4 SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 1.78 Å R-free 0.181 |
| 4PKZ Crystal structure of P1M mutant of Macrophage Migration Inhibitory Factor Deposited 2014-05-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：P1M Mutation：P1M Mutation：P1M | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 1.90 Å R-free 0.251 |
| 4PLU Crystal structure of Macrophage Migration Inhibitory Factor in complex with benzaldehyde Deposited 2014-05-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | HBX benzaldehyde × 3 SO4 SULFATE ION × 2 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5
|
Resolution 1.63 Å R-free 0.214 |
| 4TRF Crystal structure of Macrophage Migration Inhibitory Factor in complex with N-(pyridin-3-ylmethyl)thioformamide Deposited 2014-06-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 35J N-(pyridin-3-ylmethyl)thioformamide × 3 SO4 SULFATE ION × 3 GOL GLYCEROL × 2 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5
|
Resolution 1.63 Å R-free 0.181 |
| 4TRU Crystal structure of Q24A Q25A mutant of human macrophage migration inhibitory factor Deposited 2014-06-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Q24A, Q25A Mutation：Q24A, Q25A Mutation：Q24A, Q25A | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 3 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5
|
Resolution 1.81 Å R-free 0.191 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain G
2–115（114 aa）
Chain H
2–115（114 aa）
Chain I
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain J
2–115（114 aa）
Chain K
2–115（114 aa）
Chain L
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain M
2–115（114 aa）
Chain N
2–115（114 aa）
Chain O
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 8 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain P
2–115（114 aa）
Chain Q
2–115（114 aa）
Chain R
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WRB Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-190) Deposited 2014-10-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 3TW 4-{4-[6-(2-methoxyethoxy)quinolin-2-yl]-1H-1,2,3-triazol-1-yl}phenol × 1 SO4 SULFATE ION × 6 IPA ISOPROPYL ALCOHOL × 4 GOL GLYCEROL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.2M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 1.81 Å R-free 0.205 |
| 4XX7 Crystal structure of M2A mutant of human macrophage migration inhibitory factor Deposited 2015-01-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Fragment：UNP residues
Chain B
2–115（114 aa）
Fragment：UNP residues
Chain C
2–115（114 aa）
Fragment：UNP residues
|
Mutation：M2A Mutation：M2A Mutation：M2A | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 1.77 Å R-free 0.187 |
| 4XX8 Crystal structure of Pro1 deletion mutant of human macrophage migration inhibitory factor Deposited 2015-01-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
3–115（113 aa）
Chain B
3–115（113 aa）
Chain C
3–115（113 aa）
|
Mutation：Pro1 deletion Mutation：Pro1 deletion Mutation：Pro1 deletion | SO4 SULFATE ION × 5 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 1.77 Å R-free 0.188 |
| 4Z15 MIF in complex with 3-(2-furylmethyl)-2-thioxo-1,3-thiazolan-4-one Deposited 2015-03-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | IPA ISOPROPYL ALCOHOL × 1 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Tris, ammonium sulfate, isopropanol
|
Resolution 1.60 Å R-free 0.210 |
| 4Z1T MIF in complex with 4-[(4-oxo-2-thioxo-1,3-thiazolan-3-yl)methyl]benzonitrile Deposited 2015-03-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | IPA ISOPROPYL ALCOHOL × 2 SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Tris, ammonium sulfate, isopropanol
|
Resolution 1.50 Å R-free 0.225 |
| 4Z1U MIF in complex with 1-(4-methylphenyl)-3-phenylprop-2-yn-1-one Deposited 2015-03-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Tris, ammonium sulfate, isopropanol
|
Resolution 2.05 Å R-free 0.205 |
| 4Z1U MIF in complex with 1-(4-methylphenyl)-3-phenylprop-2-yn-1-one Deposited 2015-03-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 9 GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Tris, ammonium sulfate, isopropanol
|
Resolution 2.05 Å R-free 0.205 |
| 5B4O Crystal structure of Macrophage Migration Inhibitory Factor in complex with BTZO-14 Deposited 2016-04-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Fragment：UNP residues 2-115
Chain B
2–115（114 aa）
Fragment：UNP residues 2-115
Chain C
2–115（114 aa）
Fragment：UNP residues 2-115
|
Not recorded | 6DQ 2-pyridin-3-yl-1,3-benzothiazin-4-one × 4 EDO 1,2-ETHANEDIOL × 1 SO4 SULFATE ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；2.4M ammonium sulfate, 0.1M bicine pH 9.0
, 2.4 mM BTZO-14
|
Resolution 1.37 Å R-free 0.198 |
| 5BS9 Crystal structure of N109A mutant of human macrophage migration inhibitory factor Deposited 2015-06-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N109A Mutation：N109A Mutation：N109A | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 6 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.98 Å R-free 0.185 |
| 5BSC Crystal structure of K66A mutant of human macrophage migration inhibitory factor Deposited 2015-06-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：K66A Mutation：K66A Mutation：K66A | IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.89 Å R-free 0.218 |
| 5BSI Crystal structure of Y36A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y36A Mutation：Y36A Mutation：Y36A | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.221 |
| 5BSI Crystal structure of Y36A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Mutation：Y36A Mutation：Y36A Mutation：Y36A | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.221 |
| 5BSI Crystal structure of Y36A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain G
2–115（114 aa）
|
Mutation：Y36A | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.221 |
| 5BSI Crystal structure of Y36A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain J
2–115（114 aa）
|
Mutation：Y36A | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.221 |
| 5BSJ Crystal structure of S63A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：S63A Mutation：S63A Mutation：S63A | SO4 SULFATE ION × 4 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.210 |
| 5EIZ Crystal structure of Y99A mutant of human macrophage migration inhibitory factor Deposited 2015-10-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y99A Mutation：Y99A Mutation：Y99A | GOL GLYCEROL × 3 SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.96 Å R-free 0.181 |
| 5HVS Crystal Structure of Macrophage Migration Inhibitory Factor (MIF) with a Biaryltriazole Inhibitor (3i-305) Deposited 2016-01-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 65V 3-({2-[1-(3-fluoro-4-hydroxyphenyl)-1H-1,2,3-triazol-4-yl]quinolin-5-yl}oxy)benzoic acid × 3 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 8, 3% isopropanol
|
Resolution 1.75 Å R-free 0.206 |
| 5HVT Crystal Structure of Macrophage Migration Inhibitory Factor (MIF) with a Potent Inhibitor (NVS-2) Deposited 2016-01-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 NVS 7-hydroxy-3-(4-methoxyphenyl)-3,4-dihydro-2H-1,3-benzoxazin-2-one × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0 M Ammonium Sulfate, 3% isopropanol and 0.1 M Tris pH 7.0
|
Resolution 1.75 Å R-free 0.203 |
| 5HVV Crystal structure of Pro1 deletion and M2A double mutant of Macrophage Migration Inhibitory Factor Deposited 2016-01-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
3–115（113 aa）
Fragment：UNP residues 3-113
Chain B
3–115（113 aa）
Fragment：UNP residues 3-113
Chain C
3–115（113 aa）
Fragment：UNP residues 3-113
|
Mutation：M2A Mutation：M2A Mutation：M2A | SO4 SULFATE ION × 1 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.90 Å R-free 0.191 |
| 5J7P Macrophage Migration Inhibitory Factor bound to Covalent Inhibitor RDR03785 Deposited 2016-04-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 6H1 6-{[4-(trifluoromethyl)phenyl]methyl}-2H-1,3-benzodioxol-5-ol × 3 SO4 SULFATE ION × 7 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.2 M Ammonium Sulfate, 3% isopropanol and 0.1 M Tris pH 7.0
|
Resolution 1.85 Å R-free 0.201 |
| 5J7Q Macrophage Migration Inhibitory Factor bound to Inhibitor K664 Derivative Deposited 2016-04-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 6H2 4-(imidazo[1,2-a]pyridin-2-yl)benzene-1,2-diol × 1 SO4 SULFATE ION × 7 GOL GLYCEROL × 2 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.4 M Ammonium Sulfate, 3% isopropanol and 0.1 M Tris pH 7.0
|
Resolution 2.05 Å R-free 0.233 |
| 5UMJ Crystal structure of H62A mutant of human macrophage migration inhibitory factor Deposited 2017-01-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：H62A Mutation：H62A Mutation：H62A | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.61 Å R-free 0.179 |
| 5UMK Crystal structure of H62Y mutant of human macrophage migration inhibitory factor Deposited 2017-01-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：H62Y Mutation：H62Y Mutation：H62Y | SO4 SULFATE ION × 2 IPA ISOPROPYL ALCOHOL × 4 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.73 Å R-free 0.175 |
| 5UZY Crystal structure of N97A mutant of human macrophage migration inhibitory factor Deposited 2017-02-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N97A Mutation：N97A Mutation：N97A | SO4 SULFATE ION × 4 IPA ISOPROPYL ALCOHOL × 3 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.71 Å R-free 0.178 |
| 5V70 Crystal structure of N102A mutant of human macrophage migration inhibitory factor Deposited 2017-03-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N102A Mutation：N102A Mutation：N102A | SO4 SULFATE ION × 5 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.94 Å R-free 0.198 |
| 5V73 Crystal structure of N110A mutant of human macrophage migration inhibitory factor Deposited 2017-03-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N110A Mutation：N110A Mutation：N110A | SO4 SULFATE ION × 4 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.0 - 8.0
|
Resolution 1.68 Å R-free 0.175 |
| 5XEJ Crystal Structure of Macrophage Migration Inhibitory Factor bound to MTX Deposited 2017-04-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Fragment：UNP residues 2-115
Chain B
2–115（114 aa）
Fragment：UNP residues 2-115
Chain C
2–115（114 aa）
Fragment：UNP residues 2-115
|
Not recorded | SO4 SULFATE ION × 4 6UV (2~{R})-2-[[4-[[2,4-bis(azanyl)pteridin-6-yl]methyl-methyl-amino]phenyl]carbonylamino]pentanedioic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1M HEPES(pH7.5), 65% Sat. AS, 2% PEG400
|
Resolution 2.50 Å R-free 0.227 |
| 6B1C Macrophage Migration Inhibitory Factor in complex with a Naphthyridinone Inhibitor (4a) Deposited 2017-09-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 4 C9J 2-[1-(3-fluoro-4-hydroxyphenyl)-1H-1,2,3-triazol-4-yl]-7-methyl-1,7-naphthyridin-8(7H)-one × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；2.6 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 7, 3% ISOPROPANOL, 2% DMSO
|
Resolution 2.16 Å R-free 0.254 |
| 6B1K Macrophage Migration Inhibitory Factor in Complex with a Naphthyridinone Inhibitor (3a) Deposited 2017-09-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | C9G 2-[1-(4-hydroxyphenyl)-1H-1,2,3-triazol-4-yl]-7-methyl-1,7-naphthyridin-8(7H)-one × 3 SO4 SULFATE ION × 4 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293.15 K；2.1 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 7.5, 3% ISOPROPANOL, 1.4% DMSO
|
Resolution 1.17 Å R-free 0.154 |
| 6B2C Macrophage Migration Inhibitory Factor in Complex with a Naphthyridinone Inhibitor (4b) Deposited 2017-09-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 1 C9Y {2-[1-(3-fluoro-4-hydroxyphenyl)-1H-1,2,3-triazol-4-yl]-8-oxo-1,7-naphthyridin-7(8H)-yl}acetic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293.15 K；2.1 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 7.5, 3% ISOPROPANOL, 2% DMSO
|
Resolution 2.00 Å R-free 0.222 |
| 6BG6 Crystal structure of S111A mutant of human macrophage migration inhibitory factor Deposited 2017-10-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：S111A Mutation：S111A Mutation：S111A | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M Ammonium Sulphate, 3% 2-propanol, 100 mM Tris.HCl, pH 7.5
|
Resolution 1.52 Å R-free 0.174 |
| 6BG7 Crystal structure of G107A mutant of human macrophage migration inhibitory factor Deposited 2017-10-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：G107A Mutation：G107A Mutation：G107A | SO4 SULFATE ION × 4 IPA ISOPROPYL ALCOHOL × 3 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.54 Å R-free 0.225 |
| 6CB5 Macrophage Migration Inhibitory Factor in complex with a Pyrazole Inhibitor (8g) Deposited 2018-02-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | EV7 2-[(naphthalen-2-yl)oxy]-5-(1H-pyrazol-4-yl)benzoic acid × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；2.0 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 7.0, 3% ISOPROPANOL
|
Resolution 1.78 Å R-free 0.212 |
| 6CBF Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (6a) Deposited 2018-02-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | EWD 2-phenoxy-5-(1H-pyrazol-4-yl)benzoic acid × 2 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.4M ammonium sulfate, 3% isopropanol, 0.1M Tris pH 6.5
|
Resolution 2.30 Å R-free 0.282 |
| 6CBG Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (5) Deposited 2018-02-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 GOL GLYCEROL × 9 EWG 3-(1H-pyrazol-4-yl)benzoic acid × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；2.4M ammonium sulfate, 3% isopropanol, 0.1M Tris pH 7
|
Resolution 2.00 Å R-free 0.205 |
| 6CBH Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (8m) Deposited 2018-02-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 2 EWJ 5-(3-fluoro-1H-pyrazol-4-yl)-2-[(naphthalen-2-yl)oxy]benzoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.4M ammonium sulfate, 3% isopropanol, 0.1M Tris pH 7
|
Resolution 2.00 Å R-free 0.214 |
| 6FVE Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound FITC Deposited 2018-03-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 6B9 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-[(E)-(sulfanylmethylidene)amino]benzoic acid × 3 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Ammonium Sulfate, 0.1M Hepes pH 7.0
|
Resolution 1.41 Å R-free 0.189 |
| 6FVH Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound PITC Deposited 2018-03-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 0FI N-phenylthioformamide × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Ammonium Sulfate, Hepes
|
Resolution 1.40 Å R-free 0.166 |
| 6OY8 Crystal structure of Y99G mutant of human macrophage migration inhibitory factor Deposited 2019-05-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y99G Mutation：Y99G Mutation：Y99G | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.53 Å R-free 0.175 |
| 6OYB Crystal structure of H62G mutant of human macrophage migration inhibitory factor Deposited 2019-05-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：H62G Mutation：H62G Mutation：H62G | SO4 SULFATE ION × 4 GOL GLYCEROL × 5 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.53 Å R-free 0.161 |
| 6OYE Crystal structure of Y99F mutant of human macrophage migration inhibitory factor Deposited 2019-05-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y99F Mutation：Y99F Mutation：Y99F | GOL GLYCEROL × 4 SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.53 Å R-free 0.168 |
| 6OYG Crystal structure of H62F mutant of human macrophage migration inhibitory factor Deposited 2019-05-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：H62F Mutation：H62F Mutation：H62F | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.55 Å R-free 0.154 |
| 6PEG MIF with a allosteric inhibitor Deposited 2019-06-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Not recorded | ACT ACETATE ION × 4 GOL GLYCEROL × 2 SO4 SULFATE ION × 3 4FQ 4-amino-5-hydroxy-6-[(E)-(3-{[3-(2-methylpropanoyl)pyrazolo[1,5-a]pyridin-2-yl]methyl}phenyl)diazenyl]naphthalene-1,3-disulfonic acid × 1 PO4 PHOSPHATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；100microMolar 4048, 2M Ammonium sulfate, 4% isopropanol, 50 mM Tris pH 8.0, 12.5% Glycerol, 50mM sodium acetate
Protein 100mg/ml MIF in 50mM citrate pH 5, 50microMolar 4048
|
Resolution 2.00 Å R-free 0.188 |
| 7E45 Crystal structure of compound 7 bound to MIF Deposited 2021-02-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 13 HWO (2R)-7-[(2R,3R,4R,5S,6S)-6-(hydroxymethyl)-3-[(2R,3R,4R,5R,6S)-6-methyl-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,5-bis(oxidanyl)oxan-2-yl]oxy-2-(4-methoxy-3-oxidanyl-phenyl)-5-oxidanyl-2,3-dihydrochromen-4-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.0 M ammonium sulfate, 0.1 M Tris pH 7.0, 3% isopropanol
|
Resolution 1.43 Å R-free 0.186 |
| 7E47 Crystal structure of compound 6 bound to MIF Deposited 2021-02-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 7 HWR (2S,3R,4R,5R,6R)-2-[(2S,3R,4S,5R,6S)-6-(hydroxymethyl)-2-[[(2R,4R)-2-(4-hydroxyphenyl)-4,5-bis(oxidanyl)-3,4-dihydro-2H-chromen-7-yl]oxy]-4,5-bis(oxidanyl)oxan-3-yl]oxy-6-methyl-oxane-3,4,5-triol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.0M ammonium sulfate, 3% isopropanol
|
Resolution 1.38 Å R-free 0.179 |
| 7E49 Crystal structure of MIF bound to compound10 Deposited 2021-02-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 8 MYC 3,5,7-TRIHYDROXY-2-(3,4,5-TRIHYDROXYPHENYL)-4H-CHROMEN-4-ONE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.0M ammonium sulfate, 3% isopropanol
|
Resolution 1.57 Å R-free 0.235 |
| 7E4A Crystal structure of MIF bound to compound 13 Deposited 2021-02-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 11 HWL Flavoxate × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；310 K；2.0M ammonium sulfate
|
Resolution 1.48 Å R-free 0.207 |
| 7E4B Crystal structure of MIF bound to compound 5 Deposited 2021-02-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 4 MRI 2-[2,4-bis(oxidanyl)phenyl]-3,5,7-tris(oxidanyl)chromen-4-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；310 K；2.0M ammonium, 3% isopropanol
|
Resolution 1.77 Å R-free 0.232 |
| 7E4C Crystal structure of MIF bound to compound11 Deposited 2021-02-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 13 SO4 SULFATE ION × 4 GBJ 4-[(3R)-8,8-dimethyl-3,4-dihydro-2H,8H-pyrano[2,3-f]chromen-3-yl]benzene-1,3-diol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；310 K；2.0M ammonium sulfate, 3% isopropanol
|
Resolution 1.64 Å R-free 0.191 |
| 7EDQ MIF complex to compound7 Deposited 2021-03-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | HFC 6,7-bis(oxidanyl)chromen-2-one × 1 CL CHLORIDE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；290 K；2.0M Ammonium sulfate
|
Resolution 1.27 Å R-free 0.190 |
| 7EE8 The crystal structure of MIF bound to compound D5 Deposited 2021-03-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 9 7Y8 3-[(2R)-2-azanyl-1-oxidanyl-propyl]phenol × 1 J23 3-[(2S)-2-azanyl-1-oxidanyl-propyl]phenol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；310 K；2.0M Ammonium sulfate, pH7.50
|
Resolution 1.22 Å R-free 0.242 |
| 7KQX MIF Y99C homotrimeric mutant Deposited 2020-11-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y99C Mutation：Y99C Mutation：Y99C | SO4 SULFATE ION × 3 GOL GLYCEROL × 15 IPA ISOPROPYL ALCOHOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.60 Å R-free 0.180 |
| 7XTX High resolution crystal structure of human macrophage migration inhibitory factor in complex with methotrexate Deposited 2022-05-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 4 EDO 1,2-ETHANEDIOL × 11 IPA ISOPROPYL ALCOHOL × 3 MT1 N-(4-{[(2,4-DIAMINOPTERIDIN-1-IUM-6-YL)METHYL](METHYL)AMINO}BENZOYL)-L-GLUTAMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；283 K；2.0 M Ammonium sulfate
0.1 M Tris-HCl (pH 8.0)
4 % isopropanol
|
Resolution 1.28 Å R-free 0.188 |
| 7XVX Neutron crystal structure of human macrophage migration inhibitory factor Deposited 2022-05-25 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | No recorded non-water small molecule |
Experimental method not declared
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；283 K；0.49 M Monosodium phosphate, 0.91 M Dipotassium phosphate
|
Resolution not provided |
| 8CA0 Macrophage inhibitory factor (MIF) in complex with small molecule PAV174 Deposited 2023-01-24 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | VIU 1-ethyl-9-methyl-3,7-dipyrrolidin-1-yl-phenothiazine × 1 |
SOLUTION NMR
NMR measurement conditions
pH 7.2；298 K；Ionic strength (raw mmCIF value) 20；Pressure 1
NMR sample composition
1.0 mM [U-13C; U-15N] MIF, 1.0 mM PAV, 100% D2O | 100% D2O
|
Resolution not provided |
| 8IMR Structure of ligand-free human macrophage migration inhibitory factor Deposited 2023-03-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 2 EDO 1,2-ETHANEDIOL × 6 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；283 K；2.0 M Ammonium sulfate
0.1 M Tris-HCl (pH 8.0)
4 % Isopropanol
|
Resolution 1.30 Å R-free 0.217 |
| 8SON Crystal structure of macrophage migration inhibitory factor in complex with N-[3-(Trifluoromethyl)phenyl]-3-(2-chloroanilino)-2-cyano-3-thioxopropanamide Deposited 2023-04-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | GOL GLYCEROL × 3 SO4 SULFATE ION × 3 30Y (2R)-3-[(2-chlorophenyl)amino]-2-cyano-3-thioxo-N-[3-(trifluoromethyl)phenyl]propanamide × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20 mM Tris.HCl, pH 7.5, 2 M ammonium sulfate, and 3% 2-propanol
|
Resolution 1.63 Å R-free 0.199 |
| 8SPN Crystal structure of macrophage migration inhibitory factor in complex with T614 Deposited 2023-05-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：No Mutation：No Mutation：No | SO4 SULFATE ION × 2 IPA ISOPROPYL ALCOHOL × 1 GOL GLYCEROL × 1 7TN Iguratimod × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.73 Å R-free 0.202 |
| 8TT8 Joint Xray/Neutron structure of Macrophage Migration Inhibitory Factor (MIF) Bound to 4-hydroxyphenylpyruvate at room temperature Deposited 2023-08-13 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | ENO 3-(4-HYDROXY-PHENYL)PYRUVIC ACID × 2 IPA ISOPROPYL ALCOHOL × 1 EN1 (2E)-2-hydroxy-3-(4-hydroxyphenyl)prop-2-enoic acid × 1 |
Experimental method not declared
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；295 K；Protein: 1 mM MIF, 10 mM deuterated HPP, 20 mM NaCl in 20 mM Tris (pH 7.4) mixed 1:1 with reservoir containing 2M (NH4)2SO4, 1% isopropanol in 100 mM Tris (pH 7.4).
|
Resolution not provided |
| 8TT9 X-ray structure of Macrophage Migration Inhibitory Factor (MIF) Covalently Bound to 4-hydroxyphenylpyruvate (HPP) Deposited 2023-08-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | ENO 3-(4-HYDROXY-PHENYL)PYRUVIC ACID × 3 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；2M AMMONIUM SULFATE, 4% ISOPROPANOL, 0.1 M TRIS(HYDROXYMETHYL)AMINOMETHAN; MIXED WITH PROTEIN:INHIBITOR COMPLEX IN A 1:10 RATIO
|
Resolution 1.68 Å R-free 0.199 |
| 9BNQ N-(4-(isothiocyanatomethyl)phenyl)methanesulfonamide complexed with Macrophage Migration Inhibitory Factor Deposited 2024-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | A1AQU N-{[4-(methanesulfonamido)phenyl]methyl}methanethioamide × 3 SO4 SULFATE ION × 5 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289.15 K；0.2 uL 11 mg/mL MIF (100 mM Tris-HCl pH 8.0, 200 mM NaCl) were mixed with 0.2 uL of reservoir solution. Sitting drop reservoir contained 33 uL of 2.1 M Ammonium sulfate, 0.1 M Tris pH 7.5, 3% Isopropanol and <5mM N-(4-(isothiocyanatomethyl)phenyl)methanesulfonamide (10% DMSO final). Crystal was frozen in a solution of ~25% Glycerol, 75% reservoir
|
Resolution 1.09 Å R-free 0.154 |
| 9BNR 4-(2-isothiocyanatoethyl)benzenesulfonamide complexed with Macrophage Migration Inhibitory Factor Deposited 2024-05-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | A1AQT N-[2-(4-sulfamoylphenyl)ethyl]methanethioamide × 3 SO4 SULFATE ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；289.15 K；0.2 uL 11 mg/mL MIF (100 mM Tris-HCl pH 8.0, 200 mM NaCl) were mixed with 0.2 uL of reservoir solution. Sitting drop reservoir contained 33 uL of 2.0 M Ammonium sulfate, 0.1 M HEPES pH 7.0, 3% Isopropanol and <5mM 4-(2-isothiocyanatoethyl)benzenesulfonamide (10% DMSO final). Crystal was frozen in a solution of ~25% Glycerol, 75% reservoir
|
Resolution 1.53 Å R-free 0.178 |
| 9JIT Macrophage migration inhibitory factor S61H/Y100H mutant (MIF(S61H/Y100H)) Deposited 2024-09-12 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：S61H/Y100H Mutation：S61H/Y100H Mutation：S61H/Y100H | SO4 SULFATE ION × 4 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.08 Å R-free 0.155 |
| 9JIV Macrophage migration inhibitory factor Y100H mutant (MIF(Y100H)) Deposited 2024-09-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：Y100H Mutation：Y100H Mutation：Y100H | SO4 SULFATE ION × 4 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.25 Å R-free 0.152 |
| 9JIZ Macrophage migration inhibitory factor S61H/Y100H mutant complexed with three Zinc ions (Zn3-MIF(S61H/Y100H)) Deposited 2024-09-12 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：S61H/Y100H Mutation：S61H/Y100H Mutation：S61H/Y100H | ZN ZINC ION × 3 CO3 CARBONATE ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.30 Å R-free 0.167 |
| 9JJ0 Macrophage migration inhibitory factor Y100H mutant complexed with three Zinc ions (Zn3-MIF(Y100H)) Deposited 2024-09-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：Y100H Mutation：Y100H Mutation：Y100H | ZN ZINC ION × 3 CL CHLORIDE ION × 3 CO3 CARBONATE ION × 1 SO4 SULFATE ION × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.25 Å R-free 0.146 |
117 other PDB entries and 132 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | MIF_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–115； UniProt 1–115 Author chain B； PDBConstruct 1–115； UniProt 1–115 Author chain C； PDBConstruct 1–115； UniProt 1–115 |
