9paw

Cryo-EM structure of the engineered HflK/C variant stabilized in the closed conformation via disulfide bond crosslinking.

Method: ELECTRON MICROSCOPY D_max: 209.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9paw
沉积日期 deposition_date	2025-06-25
结构标题 title	Cryo-EM structure of the engineered HflK/C variant stabilized in the closed conformation via disulfide bond crosslinking.
关键词 keywords	FtsH, HflK, HflC, CHAPERONE, AAA protease; CHAPERONE
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	82.88 Å
回转半径 Rg (电子) rg_electron	83.31 Å
零角强度 I(0) i0	7789610000.00
分子量 molecular_weight	737170.0 kDa
排除体积 excluded_volume	918820 Å³
包络体积 envelope_volume	2100800 Å³
水化壳体积 shell_volume	214640 Å³
包络直径 envelope_diameter	233.5 Å
壳层 Rg shell_rg	87.59 Å
包络 Rg envelope_rg	75.27 Å
形状 Rg shape_rg	83.38 Å
总 Rg total_rg	83.11 Å
总原子数 total_atoms	103604
残基数 n_residues	6468
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	209.2 Å
Rg (实空间) rg_real	82.35 Å
Rg 误差 (实空间) rg_real_error	1.03 Å
I(0) (实空间) i0_real	7.7890e+09
I(0) 误差 (实空间) i0_real_error	1.5380e+08
Rg (倒空间) rg_reciprocal	84.40 Å
I(0) (倒空间) i0_reciprocal	7824000000.0000
解质量估计 total_estimate	0.8460
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	110.1 Å
偏度 Skewness skewness	-0.137
峰度 Kurtosis kurtosis	-0.761
角度范围 angular_range	— – 0.0950 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	199600000.0000
实空间数据点数 n_real_points	20
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 1.000; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.000

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (2)

Entity 1 polymer

描述 description	Protein HflK
分子量 formula_weight	45662.9 kDa
来源方法 src_method	man
拷贝数 entity_count	12

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	XA,XC,XE,XG,XI,XK,XM,XO,XQ,XS,XU,XW
原子数 atom_count	103604

MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVEMNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAECYTNEVQPRANGQCQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKVLGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASNTSSTSQGDIMDQRRANAQRNDYQRQGE

来源 Source

来源生物 organism	Escherichia coli
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

描述 description	Modulator of FtsH protease HflC
分子量 formula_weight	37768.0 kDa
来源方法 src_method	man
拷贝数 entity_count	12

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	XB,XD,XF,XH,XJ,XL,XN,XP,XR,XT,XV,XX

MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNKPLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSRYYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSAGTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTEVSEAIYNRMRAERECVARRHRSQGQEEAEKLRATADYEVTRTLAECERQGRIMRGEGDAEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR

来源 Source

来源生物 organism	Escherichia coli
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli BL21(DE3)

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7. 引用文献 (1)

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晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.93 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	299919

fit	/app/data/saxs_computation/pa/9paw/9paw.fit
pddf	/app/data/pr_computation/pa/9paw/9paw.pr.out
profile	/app/data/saxs_computation/pa/9paw/9paw.dat

Cryo-EM structure of the engineered HflK/C variant stabilized in the closed conformation via disulfide bond crosslinking.

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (2)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)