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9pfg

Min22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a H3:SNAP-25 SN1), 4:2:2 alphaSNAP-syntaxin-1a H3-SNAP-25 SN1 subcomplex local refinement, non-hydrolyzing, class 28

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Synaptosomal-associated protein 25

Rattus norvegicus

UniProt P60881

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 10 Syntaxin-1A × 2 （P32851） Alpha-soluble NSF attachment protein × 4 （P54921） Vesicle-fusing ATPase × 2 （P18708） water × 7 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1jth 1n7s 1sfc 1sfc 1sfc 3hd7 3hd7 3ipd 3ipd 5ccg 5ccg 5cch

49 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name SNP25_RAT
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–84； UniProt 1–83 Author chain C； PDBConstruct 2–84； UniProt 1–83

Syntaxin-1A

Rattus norvegicus

UniProt P32851

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 10 Synaptosomal-associated protein 25 × 2 （P60881） Alpha-soluble NSF attachment protein × 4 （P54921） Vesicle-fusing ATPase × 2 （P18708） water × 7 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1br0 1ez3 1ez3 1ez3 2m8r

5 assemblies

Homodimer

None

Homotetramer
1hvv

1 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex
1jth 1kil 1n7s 1sfc 1sfc 1sfc 1urq 2n1t 3c98 3hd7 3hd7 3ipd

67 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name STX1A_RAT
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 2–78； UniProt 191–267 Author chain D； PDBConstruct 2–78； UniProt 191–267

Alpha-soluble NSF attachment protein

Rattus norvegicus

UniProt P54921

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 10 Synaptosomal-associated protein 25 × 2 （P60881） Syntaxin-1A × 2 （P32851） Vesicle-fusing ATPase × 2 （P18708） water × 7 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3j96 3j97 3j98 3j99 6mdm 6mdn 9ojz 9ok3 9olo 9om6 9paf 9pag

22 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name SNAA_RAT
Isoform —
PDB entities 3
Chains and sequence ranges Author chain E； PDBConstruct 2–296； UniProt 1–295 Author chain F； PDBConstruct 2–296； UniProt 1–295 Author chain G； PDBConstruct 2–296； UniProt 1–295 Author chain H； PDBConstruct 2–296； UniProt 1–295

Vesicle-fusing ATPase

Cricetulus griseus

UniProt P18708

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 10 Synaptosomal-associated protein 25 × 2 （P60881） Syntaxin-1A × 2 （P32851） Alpha-soluble NSF attachment protein × 4 （P54921） water × 7 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1qcs

1 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers
1d2n 1nsf 1qdn 1qdn 3j94 3j95 6ip2 9nv9 9nvd 9omq 9pf2 9pfc

12 assemblies

Heteromeric or nucleic-acid complex
3j96 3j97 3j98 3j99 6mdm 6mdn 6mdo 6mdp 9ojr 9oju 9ojz 9ok5

30 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name NSF_CRIGR
Isoform —
PDB entities 4
Chains and sequence ranges Author chain J； PDBConstruct 4–747； UniProt 1–744 Author chain K； PDBConstruct 4–747； UniProt 1–744

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id9pfg
Deposition date deposition_date2025-07-04
Structure title titleMin22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a H3:SNAP-25 SN1), 4:2:2 alphaSNAP-syntaxin-1a H3-SNAP-25 SN1 subcomplex local refinement, non-hydrolyzing, class 28
Keywords keywords;ATPase, SNARE, hydrolysis, disassembly, translocation, exocytosis, neurotransmitter release, synapse, synaptic transmission, membrane fusion, HYDROLASE ;; HYDROLASE
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9pfg__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9pfg__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

9pfg__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）42.90 Å
Rg (electron density)42.75 Å
Total Rg42.96 Å
Atom count27464
Residues1760
Excluded volume246220 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 9pfg__assembly_1__model_1 decameric （10） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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7. Citations (2)