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9ptm

Antibody fragment from mAb824 bound to the adhesin protein FimH.

Method: ELECTRON MICROSCOPY D_max: 128.0 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ptm
deposition_date	2025-07-29
title	Antibody fragment from mAb824 bound to the adhesin protein FimH.
keywords	Fimbrial tip, Lectin domain, Antibody fragments, Antibody-target complex, CELL ADHESION, CELL ADHESION-IMMUNE SYSTEM complex; CELL ADHESION/IMMUNE SYSTEM
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.17 Å
rg_electron	35.29 Å
i0	90149500.00
molecular_weight	74511.0 kDa
excluded_volume	92696 Å³
envelope_volume	124470 Å³
shell_volume	32887 Å³
envelope_diameter	136.7 Å
shell_rg	37.16 Å
envelope_rg	35.52 Å
shape_rg	35.26 Å
total_rg	35.53 Å
total_atoms	5250
n_residues	696
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	128.0 Å
rg_real	35.58 Å
rg_real_error	1.53 Å
i0_real	9.0150e+07
i0_real_error	1.8100e+06
rg_reciprocal	35.32 Å
i0_reciprocal	90130000.0000
total_estimate	0.6084
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	34.1 Å
skewness	0.619
kurtosis	-0.068
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	9450000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.652; Stabil: 1.000; Sysdev: 0.186; Positv: 1.000; Valcen: 0.624; Smooth: 0.766

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Type 1 fimbrin D-mannose specific adhesin
formula_weight	31488.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5250

MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVNLAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSSYPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNSDDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYLSGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTANYARTGGQVTAGNVQSIIGVTFVYQ

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 2 polymer

description	Protein FimG
formula_weight	17328.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	G

MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 3 polymer

description	mAb824 Heavy Chain
formula_weight	22527.0 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

EIQLQQSGPERMKPGASVKISCKASGYSFTTYYIHWVKQSHGRSLEWIGYIDPFNDDTNYNQKFKGKATLTVDKSSSTAYMHLSSLTSEDSAVYYCARSYYGSLDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASST

Entity 4 polymer

description	mAb824 Light Chain
formula_weight	21757.9 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	M

DIQMTQTTSSLSASLGDRVTISCRASQGVNNYLNWYQQKPDGSVKLLIYYTSNLHSGAPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQANMVPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEAR

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.40 Å
resolution_method	—
num_particles	306269

fit	/app/data/saxs_computation/pt/9ptm/9ptm.fit
pddf	/app/data/pr_computation/pt/9ptm/9ptm.pr.out
profile	/app/data/saxs_computation/pt/9ptm/9ptm.dat

Antibody fragment from mAb824 bound to the adhesin protein FimH.

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Entity 4 polymer

Polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)