Structure of the transmembrane domain of full-length Ist2 at low resolution

Method: ELECTRON MICROSCOPY D_max: 117.3 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qja
deposition_date	2025-03-18
title	Structure of the transmembrane domain of full-length Ist2 at low resolution
keywords	lipid Transport, membrane contact sites, scramblase, TMEM16 protein, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.37 Å
rg_electron	35.53 Å
i0	147120000.00
molecular_weight	110420.0 kDa
excluded_volume	143470 Å³
envelope_volume	192640 Å³
shell_volume	45630 Å³
envelope_diameter	119.2 Å
shell_rg	41.66 Å
envelope_rg	34.90 Å
shape_rg	35.50 Å
total_rg	36.14 Å
total_atoms	7828
n_residues	950
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	117.3 Å
rg_real	36.30 Å
rg_real_error	1.09 Å
i0_real	1.4710e+08
i0_real_error	2.5200e+06
rg_reciprocal	36.35 Å
i0_reciprocal	147100000.0000
total_estimate	0.9056
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	40.9 Å
skewness	0.186
kurtosis	-0.625
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	18000000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.944; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.989; Smooth: 0.947

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	4.200 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	4.20 Å
resolution_method	—
num_particles	136329

description	Increased sodium tolerance protein 2
formula_weight	112529.8 kDa
src_method	man
entity_count	2

Structure of the transmembrane domain of full-length Ist2 at low resolution

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Saccharomyces cerevisiae
common_name	;brewer's yeast ;
ncbi_taxonomy_id	4932
host_organism	Saccharomyces cerevisiae

fit	/app/data/saxs_computation/qj/9qja/9qja.fit
pddf	/app/data/pr_computation/qj/9qja/9qja.pr.out
profile	/app/data/saxs_computation/qj/9qja/9qja.dat

type	polypeptide(L)
strand_id	B,A
atom_count	7828