SAXSdb — 9r43

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Mitomycin radical oxidase

Streptomyces lavendulae

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	FLAVIN-ADENINE DINUCLEOTIDE × 2 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 DI(HYDROXYETHYL)ETHER × 18 ACETATE ION × 8 water × 2	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 2	FLAVIN-ADENINE DINUCLEOTIDE × 2 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 DI(HYDROXYETHYL)ETHER × 14 ACETATE ION × 8 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MCRA_STRLA
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–448； UniProt 1–448 Author chain B； PDBConstruct 1–448； UniProt 1–448

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9r43
Deposition date deposition_date	2025-05-07
Last revision last_revision	2025-12-10
Structure title title	FAD-dependent oxidoreductase McrA
Keywords keywords	Flavin, FAD, natural product, oxidase, mitomycin C, OXIDOREDUCTASE; OXIDOREDUCTASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9r43__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9r43__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9r43__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	30.78 Å
Rg (electron density)	30.02 Å
Total Rg	30.63 Å
Atom count	6964
Residues	882
Excluded volume	122550 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9r43__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`9r43__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Description description	Mitomycin radical oxidase
Molecular weight formula_weight	48406.2 kDa
EC 编号 ec_number	1.5.3.-
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	7166

MSTQWGWALEPDQPGYDDARLGLNRAAESRPAYVVEAADEQEVAAAVRLAAEQKRPVGVMATGHGPSVSADDAVLVNTRRMEGVSVDAARATAWIEAGARWRKVLEHTAPHGLAPLNGSSPNVGAVGYLVGGGAGLLGRRFGYAADHVRRLRLVTADGRLRDVTAGTDPDLFWAVRGGKDNFGLVVGMEVDLFPVTRLYGGGLYFAGEATAEVLHAYAEWVRHVPEEMASSVLLVHNPDLPDVPEPLRGRFITHLRIAYSGEPADGEHLVRPLRELGPILLDTVRDMPYAEVGTIHHEPTSMPYVAYDRNVLLSDLTDDAVDIIVALAGPDAGAPFVTELRHFGGAYARPPKVPNCVGGRDAAFSLFTGAVPEAEGLRRRDDLLDRLRPWSTGGTNLNFAGVEDISPASVEAAYTPADFARLRAVKAQYDPDNMFRVNFNIPPAESWT

Source Source

Source organism organism	Streptomyces lavendulae
NCBI taxonomy ID ncbi_taxonomy_id	1914
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 non-polymer

Description description	FLAVIN-ADENINE DINUCLEOTIDE
Molecular weight formula_weight	785.5 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 3 Entity 3 non-polymer

Description description	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID
Molecular weight formula_weight	238.3 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 4 Entity 4 non-polymer

Description description	DI(HYDROXYETHYL)ETHER
Molecular weight formula_weight	106.1 kDa
Source method src_method	syn
Copy count entity_count	16

Entity 5 Entity 5 non-polymer

Description description	ACETATE ION
Molecular weight formula_weight	59.0 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 6 Entity 6 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	243

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Unit-cell length a length_a	77.872 Å
Unit-cell length b length_b	80.490 Å
Unit-cell length c length_c	163.811 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 2 2 21
Space-group number int_tables_number	17
Crystal system crystal_system	—

Resolution resolution	2.100 Å
Low-resolution limit resolution_low	72.340 Å
R factor r_factor	0.2047
R_work r_work	0.2023
R_free r_free	0.2467

FAD-dependent oxidoreductase McrA

1. Protein Identity and Related Structures Protein Identity & Related Structures

Mitomycin radical oxidase

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

9r43__assembly_1__model_1

9r43__assembly_1__model_1 | I(q)

9r43__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 water

7. Citations (1)

FAD-dependent oxidoreductase McrA

1. Protein Identity and Related Structures Protein Identity & Related Structures

Mitomycin radical oxidase

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

9r43__assembly_1__model_1

9r43__assembly_1__model_1 | I(q)

9r43__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 water

7. Citations (1)