PrPfr hybrid state of the Pseudomonas aeruginosa bacteriophytochrome / PaBphP

Method: ELECTRON MICROSCOPY D_max: 104.6 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9rcc
deposition_date	2025-05-27
title	PrPfr hybrid state of the Pseudomonas aeruginosa bacteriophytochrome / PaBphP
keywords	Phytochrome, Bacteriophytochrome, Photoreceptor, Histidine kinase, CYTOSOLIC PROTEIN; CYTOSOLIC PROTEIN
method	ELECTRON MICROSCOPY

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	34.27 Å
rg_electron	33.22 Å
i0	205640000.00
molecular_weight	112340.0 kDa
excluded_volume	139730 Å³
envelope_volume	190060 Å³
shell_volume	46873 Å³
envelope_diameter	105.1 Å
shell_rg	40.77 Å
envelope_rg	32.63 Å
shape_rg	33.22 Å
total_rg	33.82 Å
total_atoms	7910
n_residues	992
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	104.6 Å
rg_real	34.09 Å
rg_real_error	0.63 Å
i0_real	2.0560e+08
i0_real_error	2.6770e+06
rg_reciprocal	34.20 Å
i0_reciprocal	205700000.0000
total_estimate	0.9114
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	45.5 Å
skewness	0.050
kurtosis	-0.674
angular_range	— – 0.2300 Å⁻¹
current_alpha	0.0000
highest_alpha	40910000.0000
n_real_points	47
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.960; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.992; Smooth: 0.973

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (2)

Entity 1 polymer

description	Bacteriophytochrome
formula_weight	81023.7 kDa
ec_number	2.7.13.3
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	7910

MTSITPVTLANCEDEPIHVPGAIQPHGALVTLRADGMVLAASENIQALLGFVASPGSYLTQEQVGPEVLRMLEEGLTGNGPWSNSVETRIGEHLFDVIGHSYKEVFYLEFEIRTADTLSITSFTLNAQRIIAQVQLHNDTASLLSNVTDELRRMTGYDRVMAYRFRHDDSGEVVAESRREDLESYLGQRYPASDIPAQARRLYIQNPIRLIADVAYTPMRVFPALNPETNESFDLSYSVLRSVSPIHCEYLTNMGVRASMSISIVVGGKLWGLFSCHHMSPKLIPYPVRMSFQIFSQVCSAIVERLEQGRIAELLRVSTERRLALARRARDADDLFGALAHPDDGIAALIPCDGALVMLGGRTLSIRGDFERQAGNVLQRLQRDPERDIYHTDNWPQPSEDSPDGGDCCGVLAIRFHRQESGWIFWFRHEEVHRIRWGGKPEKLLTIGPSGPRLTPRGSFEAWEEVVRGHSTPWSETDLAIAEKLRLDLMELCLNHAAEVDRMRQRLIAVLGHDLRNPLQSISMAAALLSSSDTRTTELRQHISASSSRMERLVSQILDMSRLQSGIGLTVNPVDTDVSQLVRQIVCETDVAYPGLVIEIAIDPQVRAVVDPDRYAQVAANLLSNARHHGLPGRPVLVTLTRQGDEVCLSVLNETSGLSEAQLANLFEPFKRESADNQRNRNGLGIGLYISQAIAQAHQGRIDVDCRDDVITFCLRLPVRQAETGSSS

Source

organism	Pseudomonas aeruginosa
ncbi_taxonomy_id	287
host_organism	Escherichia coli BL21

Entity 2 non-polymer

description	;3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid ;
formula_weight	585.7 kDa
src_method	syn
entity_count	2

▶

6. Citations (2)

▶

length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.80 Å
resolution_method	—
num_particles	488190

fit	/app/data/saxs_computation/rc/9rcc/9rcc.fit
pddf	/app/data/pr_computation/rc/9rcc/9rcc.pr.out
profile	/app/data/saxs_computation/rc/9rcc/9rcc.dat