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9rrf

TRPC5 apo cryoEM map in the presence of pluronic acid (PA), state 2

Method: ELECTRON MICROSCOPY D_max: 142.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9rrf
deposition_date	2025-06-27
title	TRPC5 apo cryoEM map in the presence of pluronic acid (PA), state 2
keywords	Ion Channel, apo, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	45.83 Å
rg_electron	44.74 Å
i0	1303240000.00
molecular_weight	321970.0 kDa
excluded_volume	412100 Å³
envelope_volume	576130 Å³
shell_volume	102280 Å³
envelope_diameter	147.1 Å
shell_rg	53.72 Å
envelope_rg	43.39 Å
shape_rg	44.76 Å
total_rg	45.06 Å
total_atoms	23476
n_residues	2720
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	142.8 Å
rg_real	45.47 Å
rg_real_error	0.81 Å
i0_real	1.3030e+09
i0_real_error	2.0990e+07
rg_reciprocal	45.82 Å
i0_reciprocal	1304000000.0000
total_estimate	0.8827
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	58.8 Å
skewness	0.080
kurtosis	-0.437
angular_range	— – 0.1700 Å⁻¹
current_alpha	0.0000
highest_alpha	167400000.0000
n_real_points	35
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.873; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.946; Smooth: 0.905

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	Short transient receptor potential channel 5
formula_weight	89009.8 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D
atom_count	23476

MAQLYYKKVNYSPYRDRIPLQIVRAETELSAEEKAFLNAVEKGDYATVKQALQEAEIYYNVNINCMDPLGRSALLIAIENENLEIMELLLNHSVYVGDALLYAIRKEVVGAVELLLSYRRPSGEKQVPTLMMDTQFSEFTPDITPIMLAAHTNNYEIIKLLVQKRVTIPRPHQIRCNCVECVSSSEVDSLRHSRSRLNIYKALASPSLIALSSEDPILTAFRLGWELKELSKVENEFKAEYEELSQQCKLFAKDLLDQARSSRELEIILNHRDDHSEELDPQKYHDLAKLKVAIKYHQKEFVAQPNCQQLLATLWYDGFPGWRRKHWVVKLLTCMTIGFLFPMLSIAYLISPRSNLGLFIKKPFIKFICHTASYLTFLFMLLLASQHIVRTDLHVQGPPPTVVEWMILPWVLGFIWGEIKEMWDGGFTEYIHDWWNLMDFAMNSLYLATISLKIMAYVKYNGSRPREEWEMWHPTLIAEALFAISNILSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYETRAIDEPNNCKGIRCEKQNNAFSTLFETLQSLFWSVFGLLNLYVTNVKARHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADHADIEWKFARTKLWMSYFDEGGTLPPPFNIIPSPKSFLYLGNWFNNTFCPKRDPDGRRRRRNLRSFTERNADSLIQNQHYQEVIRNLVKRYVAAMIRNSKTHEGLTEENFKELKQDISSFRYEVLDLLGNRK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	(2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate
formula_weight	594.9 kDa
src_method	syn
entity_count	4

Entity 4 non-polymer

description	PHOSPHATIDYLETHANOLAMINE
formula_weight	734.0 kDa
src_method	syn
entity_count	4

Entity 5 non-polymer

description	CHOLESTEROL HEMISUCCINATE
formula_weight	486.7 kDa
src_method	syn
entity_count	4

Entity 6 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	4

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.300 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.30 Å
resolution_method	—
num_particles	167808

fit	/app/data/saxs_computation/rr/9rrf/9rrf.fit
pddf	/app/data/pr_computation/rr/9rrf/9rrf.pr.out
profile	/app/data/saxs_computation/rr/9rrf/9rrf.dat