7x6i

Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class1

Method: ELECTRON MICROSCOPY D_max: 197.5 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Short transient receptor potential channel 5

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count	Chain A； UniProt 1–765 Chain B； UniProt 1–765 Chain C； UniProt 1–765 Chain D； UniProt 1–765	Not recorded	Guanine nucleotide-binding protein G(i) subunit alpha-3 × 4 （P08754） PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 3.93 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 7X6I	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
6YSN Human TRPC5 in complex with Pico145 (HC-608) Deposited 2020-04-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PJQ 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-[3-(trifluoromethyloxy)phenoxy]purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7D4P Structure of human TRPC5 in complex with clemizole Deposited 2020-09-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	GX0 1-[(4-chlorophenyl)methyl]-2-(pyrrolidin-1-ylmethyl)benzimidazole × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
7D4Q Structure of human TRPC5 in complex with HC-070 Deposited 2020-09-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 GWR 8-(3-chloranylphenoxy)-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.74 Å
7E4T Human TRPC5 apo state structure at 3 angstrom Deposited 2021-02-15	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7WDB Human TRPC5 channel in complex with riluzole Deposited 2021-12-21	Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 A1L8M [(2S)-1-oxidanyl-3-[(E)-tetradec-9-enoyl]oxy-propan-2-yl] (Z)-pentadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
7X6C Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class1 Deposited 2022-03-07	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.15 Å
8GVW Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class2 Deposited 2022-09-16	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.59 Å
8GVX Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class2 Deposited 2022-09-16	Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.91 Å
9G4Y TRPC5 in complex with photoswitch E-AzHC Deposited 2024-07-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	A1IIE 8-[3-chloranyl-4-[(~{E})-phenyldiazenyl]phenoxy]-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9G50 TRPC5 in complex with photoswitch Z-AzHC Deposited 2024-07-16	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4 A1IIF 8-[3-chloranyl-4-[(~{Z})-phenyldiazenyl]phenoxy]-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9GKB TRPC5 in complex with spin-labelled ligand SpinPico1 Deposited 2024-08-23	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	A1IMO 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-(2,2,6,6-tetramethyl-1-oxidanyl-piperidin-4-yl)oxy-purine-2,6-dione × 4 CA CALCIUM ION × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.32 Å
9GL6 TRPC5 in complex with spin-labelled ligand SpinPico3 Deposited 2024-08-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	Y01 CHOLESTEROL HEMISUCCINATE × 4 CA CALCIUM ION × 4 A1IMP 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-(1,1,3,3-tetramethyl-2-oxidanyl-isoindol-5-yl)oxy-purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9KHI Cryo-EM structure of heteromeric TRPC channel Deposited 2024-11-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 3 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 3 Y01 CHOLESTEROL HEMISUCCINATE × 3 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9KHJ Cryo-EM structure of homomeric TRPC channel, class 1 Deposited 2024-11-10	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
9KHK Cryo-EM structure of homomeric TRPC channel, class 2 Deposited 2024-11-10	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9LZY Cryo-EM structure of homomeric TRPC channel with agonists, class 1 Deposited 2025-02-22	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 A1L55 (-)-englerin A × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
9LZZ Cryo-EM structure of homomeric TRPC channel with agonists, class 2 Deposited 2025-02-22	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 A1L55 (-)-englerin A × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.03 Å
9M36 Structure of human TRPC5 in the low Ca environment. Deposited 2025-02-28	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L5I [(2~{S})-2-[(~{E})-octadec-9-enoyl]oxy-3-oxidanyl-propyl] octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.48 Å
9M4W Structure of human TRPC5 bound with (-)-englerin A, class 2. Deposited 2025-03-05	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
9M5V Structure of human TRPC5 bound with (-)-englerin A,class1 Deposited 2025-03-06	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–973（973 aa） Chain B 1–973（973 aa） Chain C 1–973（973 aa） Chain D 1–973（973 aa）	Not recorded	Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.53 Å
9RRF TRPC5 apo cryoEM map in the presence of pluronic acid (PA), state 2 Deposited 2025-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.30 Å
9RRM Human TRPC5 in complex with (-) englerin A, full occupancy, state 1, on 290 nm gold foil holes (HexAuFoil) Deposited 2025-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 4 ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
9RRN Human TRPC5 in complex with (-) englerin A, full occupancy, state 2, on 290 nm gold foil holes (HexAuFoil) Deposited 2025-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
9RRO Human TRPC5 in complex with (-) englerin A, full occupancy, intermediary desensitized state Deposited 2025-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 4 ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
9RRQ Human TRPC5 in complex with (-) englerin A, partial occupancy (2EA:2LIP stoichiometry) state 1 Deposited 2025-06-27	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 2 ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9RRU Human TRPC5 in complex with (-) englerin A, mixed occupancy, state 1 Deposited 2025-06-30	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9RSG Human TRPC5 in complex with (-) englerin A, mixed occupancy_2, state 2 Deposited 2025-07-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9RSH Human TRPC5 in complex with (-) englerin A, mixed occupancy, state 2 Deposited 2025-07-01	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
9RVV Human TRPC5 in complex with (-) englerin A, partial occupancy (2EA:2LIP stoichiometry) state 2 Deposited 2025-07-09	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 2 ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å

29 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	TRPC5_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–765； UniProt 1–765 Author chain B； PDBConstruct 1–765； UniProt 1–765 Author chain C； PDBConstruct 1–765； UniProt 1–765 Author chain D； PDBConstruct 1–765； UniProt 1–765

Guanine nucleotide-binding protein G(i) subunit alpha-3

Homo sapiens

UniProt P08754

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count	Chain E； UniProt 1–119 Chain E； UniProt 120–354 Chain F； UniProt 1–119 Chain F； UniProt 120–354 Chain G； UniProt 1–119 Chain G； UniProt 120–354 Chain H； UniProt 1–119 Chain H； UniProt 120–354	Mutation：Q204L	Short transient receptor potential channel 5 × 4 （Q9UL62） PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 3.93 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7X6I	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2IHB Crystal structure of the heterodimeric complex of human RGS10 and activated Gi alpha 3 Deposited 2006-09-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 32–354（323 aa）	Not recorded	ALF TETRAFLUOROALUMINATE ION × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；293 K；0.2M (NH4)2SO4, 0.1M Bis-Tris, pH 5.5, 25% PEG3350 , VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.71 Å R-free 0.236
2ODE Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3 Deposited 2006-12-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 4–350（347 aa）	Not recorded	ALF TETRAFLUOROALUMINATE ION × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.20M NH4Cl, 20.0% PEG 6K, 10.0% EtGly, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 1.90 Å R-free 0.211
2ODE Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3 Deposited 2006-12-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 4–350（347 aa）	Not recorded	ALF TETRAFLUOROALUMINATE ION × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.20M NH4Cl, 20.0% PEG 6K, 10.0% EtGly, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 1.90 Å R-free 0.211
2V4Z The crystal structure of the human G-protein subunit alpha (GNAI3) in complex with an engineered regulator of G-protein signaling type 2 domain (RGS2) Deposited 2008-09-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 4–350（347 aa） Fragment：SUBUNIT ALPHA, RESIDUES 4-350	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 ALF TETRAFLUOROALUMINATE ION × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.5；0.1 M HEPES PH 7.5, 2 M AMMONIUM SULPHATE	Resolution 2.80 Å R-free 0.254
4G5O Structure of LGN GL4/Galphai3(Q147L) complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Mutation：Q147L	GDP GUANOSINE-5'-DIPHOSPHATE × 1 CIT CITRIC ACID × 2 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.90 Å R-free 0.244
4G5O Structure of LGN GL4/Galphai3(Q147L) complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Mutation：Q147L	GDP GUANOSINE-5'-DIPHOSPHATE × 1 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.90 Å R-free 0.244
4G5O Structure of LGN GL4/Galphai3(Q147L) complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Mutation：Q147L	GDP GUANOSINE-5'-DIPHOSPHATE × 1 CIT CITRIC ACID × 1 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.90 Å R-free 0.244
4G5O Structure of LGN GL4/Galphai3(Q147L) complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Mutation：Q147L	GDP GUANOSINE-5'-DIPHOSPHATE × 1 CIT CITRIC ACID × 1 SO4 SULFATE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.90 Å R-free 0.244
4G5R Structure of LGN GL4/Galphai3 complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.48 Å R-free 0.253
4G5R Structure of LGN GL4/Galphai3 complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 SO4 SULFATE ION × 1 CIT CITRIC ACID × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.48 Å R-free 0.253
4G5R Structure of LGN GL4/Galphai3 complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 SO4 SULFATE ION × 1 CIT CITRIC ACID × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.48 Å R-free 0.253
4G5R Structure of LGN GL4/Galphai3 complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.48 Å R-free 0.253
4G5S Structure of LGN GL3/Galphai3 complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 CIT CITRIC ACID × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.62 Å R-free 0.253
4G5S Structure of LGN GL3/Galphai3 complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.62 Å R-free 0.253
4G5S Structure of LGN GL3/Galphai3 complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1 CIT CITRIC ACID × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.62 Å R-free 0.253
4G5S Structure of LGN GL3/Galphai3 complex Deposited 2012-07-18	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 25–354（330 aa） Fragment：UNP RESIDUES 25-354	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.62 Å R-free 0.253
7E9H Cryo-EM structure of Gi-bound metabotropic glutamate receptor mGlu4 Deposited 2021-03-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–354（354 aa）	Mutation：S47N, F191D, G203A, E245A, A326S	SEP PHOSPHOSERINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 4.00 Å
7KH0 Cryo-EM structure of the human arginine vasopressin AVP-vasopressin receptor V2R-Gs signaling complex Deposited 2020-10-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 7 PDB declaration: heptameric	Chain A 2–18（17 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
7RA3 cryo-EM of human Gastric inhibitory polypeptide receptor GIPR bound to GIP Deposited 2021-06-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 7 PDB declaration: heptameric	Chain A 1–18（18 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.24 Å
7RGP cryo-EM of human Glucagon-like peptide 1 receptor GLP-1R bound to tirzepatide Deposited 2021-07-15	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 7 PDB declaration: heptameric	Chain A 1–18（18 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7T10 CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3 Deposited 2021-11-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–354（354 aa）	Mutation：S47N, G203A, E245A, A326S	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
7T11 CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3. Deposited 2021-11-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–354（354 aa）	Mutation：S47N, G203A, E245A, A326S	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
8GVX Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class2 Deposited 2022-09-16	Different construct Different mutation/modification Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain E 1–354（354 aa） Chain F 1–354（354 aa） Chain G 1–354（354 aa） Chain H 1–354（354 aa）	Mutation：Q204L Mutation：Q204L Mutation：Q204L Mutation：Q204L	PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.91 Å
8GY7 Cryo-EM structure of ACTH-bound melanocortin-2 receptor in complex with MRAP1 and Gs protein Deposited 2022-09-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–230（230 aa）	Not recorded	CA CALCIUM ION × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.2 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å
8JD6 Cryo-EM structure of Gi1-bound metabotropic glutamate receptor mGlu4 Deposited 2023-05-12	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–354（354 aa）	Not recorded	BK0 N-(3-chlorophenyl)pyridine-2-carboxamide × 1 SEP PHOSPHOSERINE × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
8OY1 Structure of the human Guanine Nucleotide-Binding Protein G(K) Subunit Alpha Deposited 2023-05-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 31–350（320 aa）	Not recorded	GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；297 K；0.1 M sodium MES at pH 6.5 containing 20% PEG 8000 and 0.2 M ammonium sulfate	Resolution 3.34 Å R-free 0.280
8SZH Cryo-EM structure of cinacalcet-bound human calcium-sensing receptor CaSR-Gi complex in lipid nanodiscs Deposited 2023-05-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain C 1–354（354 aa）	Not recorded	YP4 N-[(1R)-1-(naphthalen-1-yl)ethyl]-3-[3-(trifluoromethyl)phenyl]propan-1-amine × 2 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 CA CALCIUM ION × 4 TRP TRYPTOPHAN × 2 PO4 PHOSPHATE ION × 2 SPM SPERMINE × 4 CLR CHOLESTEROL × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.10 Å
8SZI Cryo-EM structure of PAM-free human calcium-sensing receptor CaSR-Gi complex in lipid nanodiscs Deposited 2023-05-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain C 1–354（354 aa）	Mutation：S47N, G203A, E245A, A326S	PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 CA CALCIUM ION × 4 TRP TRYPTOPHAN × 3 PO4 PHOSPHATE ION × 2 CLR CHOLESTEROL × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.50 Å
8WGB mGlu2-4 heterodimer bound with Gi Deposited 2023-09-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric	Chain C 1–354（354 aa）	Not recorded	GLU GLUTAMIC ACID × 2 W9R (1R,2S)-2-[[3,5-bis(chloranyl)phenyl]carbamoyl]cyclohexane-1-carboxylic acid × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.70 Å
8YK0 Cryo-EM structure of human GPR156-Gi3 complex Deposited 2024-03-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric	Chain C 6–354（349 aa）	Not recorded	MW9 (21R,24R,27S)-24,27,28-trihydroxy-18,24-dioxo-19,23,25-trioxa-24lambda~5~-phosphaoctacosan-21-yl (9Z)-octadec-9-enoate × 4 CLR CHOLESTEROL × 11	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
9AVL Structure of human calcium-sensing receptor in complex with Gi3 protein in nanodiscs Deposited 2024-03-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain A 1–354（354 aa）	Mutation：S47N, G203A, E245A, A326S	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 TCR CYCLOMETHYLTRYPTOPHAN × 2 PO4 PHOSPHATE ION × 2 CA CALCIUM ION × 6 9IG 3-(2-chlorophenyl)-N-[(1R)-1-(3-methoxyphenyl)ethyl]propan-1-amine × 2 Y01 CHOLESTEROL HEMISUCCINATE × 1 A1AF7 (19R,22S,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9Z)-octadec-9-enoate × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE；The sample was blotted for 6s before plunge-frozen.	Resolution 3.80 Å
9VJ6 Cryo-EM structure of 5-HT1AR-Gi3 in complex with F-15599 Deposited 2025-06-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric	Chain B 1–354（354 aa）	Not recorded	A1ESC (3-chloranyl-4-fluoranyl-phenyl)-[4-fluoranyl-4-[[(5-methylpyrimidin-2-yl)methylamino]methyl]piperidin-1-yl]methanone × 1 CLR CHOLESTEROL × 2 T7M (2R)-1-(heptadecanoyloxy)-3-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
9VJF Cryo-EM structure of 5-HT1AR-Gi3 in complex with buspirone Deposited 2025-06-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric	Chain B 1–354（354 aa）	Not recorded	CLR CHOLESTEROL × 2 T7M (2R)-1-(heptadecanoyloxy)-3-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate × 1 YLX Buspirone × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9WXM Cryo-EM structure of the full-length GPR15L bound GPR15-Gi complex Deposited 2025-09-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric	Chain A 1–354（354 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.30 Å

24 other PDB entries and 34 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	GNAI3_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain E； PDBConstruct 1–119； UniProt 1–119 Author chain E； PDBConstruct 126–360； UniProt 120–354 Author chain F； PDBConstruct 1–119； UniProt 1–119 Author chain F； PDBConstruct 126–360； UniProt 120–354 Author chain G； PDBConstruct 1–119； UniProt 1–119 Author chain G； PDBConstruct 126–360； UniProt 120–354 Author chain H； PDBConstruct 1–119； UniProt 1–119 Author chain H； PDBConstruct 126–360； UniProt 120–354

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7x6i
Deposition date deposition_date	2022-03-07
Structure title title	Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class1
Keywords keywords	transient receptor potential, METAL TRANSPORT; METAL TRANSPORT
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	59.46 Å
Radius of gyration Rg (electron density) rg_electron	58.90 Å
Forward intensity I(0) i0	2830940000.00
Molecular weight molecular_weight	467100.0 kDa
Excluded volume excluded_volume	592890 Å³
Envelope volume envelope_volume	884830 Å³
Hydration-shell volume shell_volume	127010 Å³
Envelope diameter envelope_diameter	190.4 Å
Shell Rg shell_rg	59.47 Å
Envelope Rg envelope_rg	57.43 Å
Shape Rg shape_rg	58.93 Å
Total Rg total_rg	58.82 Å
Total atoms total_atoms	32908
Residues n_residues	3968
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	197.5 Å
Rg (real space) rg_real	59.28 Å
Rg uncertainty (real space) rg_real_error	1.82 Å
I(0) (real space) i0_real	2.8310e+09
I(0) uncertainty (real space) i0_real_error	6.2100e+07
Rg (reciprocal space) rg_reciprocal	59.60 Å
I(0) (reciprocal space) i0_reciprocal	2832000000.0000
Solution quality estimate total_estimate	0.8688
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	76.0 Å
Skewness Skewness skewness	0.216
Kurtosis Kurtosis kurtosis	-0.428
Angular range angular_range	— – 0.1300 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	132700000.0000
Real-space data points n_real_points	27
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.858; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.964; Smooth: 0.752

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Description description	Short transient receptor potential channel 5
Molecular weight formula_weight	89951.9 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	32908

MAQLYYKKVNYSPYRDRIPLQIVRAETELSAEEKAFLNAVEKGDYATVKQALQEAEIYYNVNINCMDPLGRSALLIAIENENLEIMELLLNHSVYVGDALLYAIRKEVVGAVELLLSYRRPSGEKQVPTLMMDTQFSEFTPDITPIMLAAHTNNYEIIKLLVQKRVTIPRPHQIRCNCVECVSSSEVDSLRHSRSRLNIYKALASPSLIALSSEDPILTAFRLGWELKELSKVENEFKAEYEELSQQCKLFAKDLLDQARSSRELEIILNHRDDHSEELDPQKYHDLAKLKVAIKYHQKEFVAQPNCQQLLATLWYDGFPGWRRKHWVVKLLTCMTIGFLFPMLSIAYLISPRSNLGLFIKKPFIKFICHTASYLTFLFMLLLASQHIVRTDLHVQGPPPTVVEWMILPWVLGFIWGEIKEMWDGGFTEYIHDWWNLMDFAMNSLYLATISLKIVAYVKYNGSRPREEWEMWHPTLIAEALFAISNILSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYETRAIDEPNNCKGIRCEKQNNAFSTLFETLQSLFWSVFGLLNLYVTNVKARHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADHADIEWKFARTKLWMSYFDEGGTLPPPFNIIPSPKSFLYLGNWFNNTFCPKRDPDGRRRRRNLRSFTERNADSLIQNQHYQEVIRNLVKRYVAAMIRNSKTHEGLTEENFKELKQDISSFRYEVLDLLGNRKSRLEVLFQ

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 2 Entity 2 polymer

Description description	Guanine nucleotide-binding protein G(i) subunit alpha-3
Molecular weight formula_weight	41399.0 kDa
Mutation mutation	Q204L
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E,F,G,H

MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMHHHHHHTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGLRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli K-12

Entity 3 Entity 3 non-polymer

Description description	PHOSPHATIDYLETHANOLAMINE
Molecular weight formula_weight	734.0 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 4 Entity 4 non-polymer

Description description	CHOLESTEROL HEMISUCCINATE
Molecular weight formula_weight	486.7 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 5 Entity 5 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 6 Entity 6 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 7 Entity 7 non-polymer

Description description	(2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate
Molecular weight formula_weight	594.9 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 8 Entity 8 non-polymer

Description description	GUANOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	523.2 kDa
Source method src_method	syn
Copy count entity_count	4

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8. Citations (1)

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Resolution resolution	3.93 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	18935

fit	/app/data/saxs_computation/x6/7x6i/7x6i.fit
pddf	/app/data/pr_computation/x6/7x6i/7x6i.pr.out
profile	/app/data/saxs_computation/x6/7x6i/7x6i.dat

Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class1

1. Protein Identity and Related Structures Protein Identity & Related Structures

Short transient receptor potential channel 5

State in the Current Structure

Other States of the Same Protein in the Database

Guanine nucleotide-binding protein G(i) subunit alpha-3

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 polymer

Polymer Polymer

来源 Source

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 non-polymer

Entity 8 Entity 8 non-polymer

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)