9lzz

Cryo-EM structure of homomeric TRPC channel with agonists, class 2

Method: ELECTRON MICROSCOPY D_max: 140.7 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Short transient receptor potential channel 5

Homo sapiens

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 1–765 Chain B； UniProt 1–765 Chain C； UniProt 1–765 Chain D； UniProt 1–765	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 A1L55 (-)-englerin A × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4	ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 3.03 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 9LZZ	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
6YSN Human TRPC5 in complex with Pico145 (HC-608) Deposited 2020-04-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PJQ 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-[3-(trifluoromethyloxy)phenoxy]purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7D4P Structure of human TRPC5 in complex with clemizole Deposited 2020-09-24	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	GX0 1-[(4-chlorophenyl)methyl]-2-(pyrrolidin-1-ylmethyl)benzimidazole × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
7D4Q Structure of human TRPC5 in complex with HC-070 Deposited 2020-09-24	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 GWR 8-(3-chloranylphenoxy)-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.74 Å
7E4T Human TRPC5 apo state structure at 3 angstrom Deposited 2021-02-15	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
7WDB Human TRPC5 channel in complex with riluzole Deposited 2021-12-21	Different construct Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 A1L8M [(2S)-1-oxidanyl-3-[(E)-tetradec-9-enoyl]oxy-propan-2-yl] (Z)-pentadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.40 Å
7X6C Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class1 Deposited 2022-03-07	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.15 Å
7X6I Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class1 Deposited 2022-03-07	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.93 Å
8GVW Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class2 Deposited 2022-09-16	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.59 Å
8GVX Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class2 Deposited 2022-09-16	Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.91 Å
9G4Y TRPC5 in complex with photoswitch E-AzHC Deposited 2024-07-16	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	A1IIE 8-[3-chloranyl-4-[(~{E})-phenyldiazenyl]phenoxy]-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9G50 TRPC5 in complex with photoswitch Z-AzHC Deposited 2024-07-16	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4 A1IIF 8-[3-chloranyl-4-[(~{Z})-phenyldiazenyl]phenoxy]-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9GKB TRPC5 in complex with spin-labelled ligand SpinPico1 Deposited 2024-08-23	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	A1IMO 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-(2,2,6,6-tetramethyl-1-oxidanyl-piperidin-4-yl)oxy-purine-2,6-dione × 4 CA CALCIUM ION × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.32 Å
9GL6 TRPC5 in complex with spin-labelled ligand SpinPico3 Deposited 2024-08-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	Y01 CHOLESTEROL HEMISUCCINATE × 4 CA CALCIUM ION × 4 A1IMP 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-(1,1,3,3-tetramethyl-2-oxidanyl-isoindol-5-yl)oxy-purine-2,6-dione × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
9KHI Cryo-EM structure of heteromeric TRPC channel Deposited 2024-11-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 3 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 3 Y01 CHOLESTEROL HEMISUCCINATE × 3 ZN ZINC ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
9KHJ Cryo-EM structure of homomeric TRPC channel, class 1 Deposited 2024-11-10	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
9KHK Cryo-EM structure of homomeric TRPC channel, class 2 Deposited 2024-11-10	Different ligand/ion Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9LZY Cryo-EM structure of homomeric TRPC channel with agonists, class 1 Deposited 2025-02-22	Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 A1L55 (-)-englerin A × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.43 Å
9M36 Structure of human TRPC5 in the low Ca environment. Deposited 2025-02-28	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L5I [(2~{S})-2-[(~{E})-octadec-9-enoyl]oxy-3-oxidanyl-propyl] octadec-9-enoate × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.48 Å
9M4W Structure of human TRPC5 bound with (-)-englerin A, class 2. Deposited 2025-03-05	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–764（764 aa） Chain B 1–764（764 aa） Chain C 1–764（764 aa） Chain D 1–764（764 aa）	Not recorded	ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.62 Å
9M5V Structure of human TRPC5 bound with (-)-englerin A,class1 Deposited 2025-03-06	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–973（973 aa） Chain B 1–973（973 aa） Chain C 1–973（973 aa） Chain D 1–973（973 aa）	Not recorded	Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.53 Å
9RRF TRPC5 apo cryoEM map in the presence of pluronic acid (PA), state 2 Deposited 2025-06-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 CA CALCIUM ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.30 Å
9RRM Human TRPC5 in complex with (-) englerin A, full occupancy, state 1, on 290 nm gold foil holes (HexAuFoil) Deposited 2025-06-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 4 ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
9RRN Human TRPC5 in complex with (-) englerin A, full occupancy, state 2, on 290 nm gold foil holes (HexAuFoil) Deposited 2025-06-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
9RRO Human TRPC5 in complex with (-) englerin A, full occupancy, intermediary desensitized state Deposited 2025-06-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 4 ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
9RRQ Human TRPC5 in complex with (-) englerin A, partial occupancy (2EA:2LIP stoichiometry) state 1 Deposited 2025-06-27	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 2 ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
9RRU Human TRPC5 in complex with (-) englerin A, mixed occupancy, state 1 Deposited 2025-06-30	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9RSG Human TRPC5 in complex with (-) englerin A, mixed occupancy_2, state 2 Deposited 2025-07-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å
9RSH Human TRPC5 in complex with (-) englerin A, mixed occupancy, state 2 Deposited 2025-07-01	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	ZN ZINC ION × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å
9RVV Human TRPC5 in complex with (-) englerin A, partial occupancy (2EA:2LIP stoichiometry) state 2 Deposited 2025-07-09	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1–765（765 aa） Chain B 1–765（765 aa） Chain C 1–765（765 aa） Chain D 1–765（765 aa）	Not recorded	CA CALCIUM ION × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 2 ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å

29 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	TRPC5_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–765； UniProt 1–765 Author chain B； PDBConstruct 1–765； UniProt 1–765 Author chain C； PDBConstruct 1–765； UniProt 1–765 Author chain D； PDBConstruct 1–765； UniProt 1–765

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9lzz
Deposition date deposition_date	2025-02-22
Structure title title	Cryo-EM structure of homomeric TRPC channel with agonists, class 2
Keywords keywords	TRP, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	45.37 Å
Radius of gyration Rg (electron density) rg_electron	44.65 Å
Forward intensity I(0) i0	2753310000.00
Molecular weight molecular_weight	299930.0 kDa
Excluded volume excluded_volume	295710 Å³
Envelope volume envelope_volume	576100 Å³
Hydration-shell volume shell_volume	102340 Å³
Envelope diameter envelope_diameter	143.1 Å
Shell Rg shell_rg	53.81 Å
Envelope Rg envelope_rg	43.29 Å
Shape Rg shape_rg	44.65 Å
Total Rg total_rg	44.94 Å
Total atoms total_atoms	22772
Residues n_residues	2712
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	140.7 Å
Rg (real space) rg_real	45.04 Å
Rg uncertainty (real space) rg_real_error	0.76 Å
I(0) (real space) i0_real	2.7530e+09
I(0) uncertainty (real space) i0_real_error	4.4470e+07
Rg (reciprocal space) rg_reciprocal	45.37 Å
I(0) (reciprocal space) i0_reciprocal	2754000000.0000
Solution quality estimate total_estimate	0.8862
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	60.3 Å
Skewness Skewness skewness	0.085
Kurtosis Kurtosis kurtosis	-0.460
Angular range angular_range	— – 0.1750 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	175500000.0000
Real-space data points n_real_points	36
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.888; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.963; Smooth: 0.889

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (8)

Entity 1 Entity 1 polymer

Description description	Short transient receptor potential channel 5
Molecular weight formula_weight	89951.9 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,D,C,B
Atom count atom_count	22772

MAQLYYKKVNYSPYRDRIPLQIVRAETELSAEEKAFLNAVEKGDYATVKQALQEAEIYYNVNINCMDPLGRSALLIAIENENLEIMELLLNHSVYVGDALLYAIRKEVVGAVELLLSYRRPSGEKQVPTLMMDTQFSEFTPDITPIMLAAHTNNYEIIKLLVQKRVTIPRPHQIRCNCVECVSSSEVDSLRHSRSRLNIYKALASPSLIALSSEDPILTAFRLGWELKELSKVENEFKAEYEELSQQCKLFAKDLLDQARSSRELEIILNHRDDHSEELDPQKYHDLAKLKVAIKYHQKEFVAQPNCQQLLATLWYDGFPGWRRKHWVVKLLTCMTIGFLFPMLSIAYLISPRSNLGLFIKKPFIKFICHTASYLTFLFMLLLASQHIVRTDLHVQGPPPTVVEWMILPWVLGFIWGEIKEMWDGGFTEYIHDWWNLMDFAMNSLYLATISLKIVAYVKYNGSRPREEWEMWHPTLIAEALFAISNILSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYETRAIDEPNNCKGIRCEKQNNAFSTLFETLQSLFWSVFGLLNLYVTNVKARHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADHADIEWKFARTKLWMSYFDEGGTLPPPFNIIPSPKSFLYLGNWFNNTFCPKRDPDGRRRRRNLRSFTERNADSLIQNQHYQEVIRNLVKRYVAAMIRNSKTHEGLTEENFKELKQDISSFRYEVLDLLGNRKSRLEVLFQ

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 2 Entity 2 non-polymer

Description description	PHOSPHATIDYLETHANOLAMINE
Molecular weight formula_weight	734.0 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 3 Entity 3 non-polymer

Description description	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate
Molecular weight formula_weight	760.1 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 4 Entity 4 non-polymer

Description description	CHOLESTEROL HEMISUCCINATE
Molecular weight formula_weight	486.7 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 5 Entity 5 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 6 Entity 6 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 7 Entity 7 non-polymer

Description description	(-)-englerin A
Molecular weight formula_weight	442.5 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 8 Entity 8 non-polymer

Description description	6-(trifluoromethoxy)-1,3-benzothiazol-2-amine
Molecular weight formula_weight	234.2 kDa
Source method src_method	syn
Copy count entity_count	4

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.030 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	3.03 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	22419

fit	/app/data/saxs_computation/lz/9lzz/9lzz.fit
pddf	/app/data/pr_computation/lz/9lzz/9lzz.pr.out
profile	/app/data/saxs_computation/lz/9lzz/9lzz.dat