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9rsg

Human TRPC5 in complex with (-) englerin A, mixed occupancy_2, state 2

Method: ELECTRON MICROSCOPY Dmax: 131.5 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Short transient receptor potential channel 5

Homo sapiens

UniProt Q9UL62

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count Chain A； UniProt 1–765 Chain B； UniProt 1–765 Chain C； UniProt 1–765 Chain D； UniProt 1–765 Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9RSG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6YSN Human TRPC5 in complex with Pico145 (HC-608) Deposited 2020-04-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PJQ 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-[3-(trifluoromethyloxy)phenoxy]purine-2,6-dione × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7D4P Structure of human TRPC5 in complex with clemizole Deposited 2020-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–764（764 aa）
Chain B 1–764（764 aa）
Chain C 1–764（764 aa）
Chain D 1–764（764 aa）
Not recorded GX0 1-[(4-chlorophenyl)methyl]-2-(pyrrolidin-1-ylmethyl)benzimidazole × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7D4Q Structure of human TRPC5 in complex with HC-070 Deposited 2020-09-24 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–764（764 aa）
Chain B 1–764（764 aa）
Chain C 1–764（764 aa）
Chain D 1–764（764 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 GWR 8-(3-chloranylphenoxy)-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.74 Å
7E4T Human TRPC5 apo state structure at 3 angstrom Deposited 2021-02-15 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–764（764 aa）
Chain B 1–764（764 aa）
Chain C 1–764（764 aa）
Chain D 1–764（764 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7WDB Human TRPC5 channel in complex with riluzole Deposited 2021-12-21 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–764（764 aa）
Chain B 1–764（764 aa）
Chain C 1–764（764 aa）
Chain D 1–764（764 aa）
Not recorded POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 A1L8M [(2S)-1-oxidanyl-3-[(E)-tetradec-9-enoyl]oxy-propan-2-yl] (Z)-pentadec-9-enoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
7X6C Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class1 Deposited 2022-03-07 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.15 Å
7X6I Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class1 Deposited 2022-03-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.93 Å
8GVW Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class2 Deposited 2022-09-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.59 Å
8GVX Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class2 Deposited 2022-09-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.91 Å
9G4Y TRPC5 in complex with photoswitch E-AzHC Deposited 2024-07-16 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded A1IIE 8-[3-chloranyl-4-[(~{E})-phenyldiazenyl]phenoxy]-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9G50 TRPC5 in complex with photoswitch Z-AzHC Deposited 2024-07-16 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded ZN ZINC ION × 4 A1IIF 8-[3-chloranyl-4-[(~{Z})-phenyldiazenyl]phenoxy]-7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)purine-2,6-dione × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9GKB TRPC5 in complex with spin-labelled ligand SpinPico1 Deposited 2024-08-23 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded A1IMO 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-(2,2,6,6-tetramethyl-1-oxidanyl-piperidin-4-yl)oxy-purine-2,6-dione × 4 CA CALCIUM ION × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.32 Å
9GL6 TRPC5 in complex with spin-labelled ligand SpinPico3 Deposited 2024-08-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded Y01 CHOLESTEROL HEMISUCCINATE × 4 CA CALCIUM ION × 4 A1IMP 7-[(4-chlorophenyl)methyl]-3-methyl-1-(3-oxidanylpropyl)-8-(1,1,3,3-tetramethyl-2-oxidanyl-isoindol-5-yl)oxy-purine-2,6-dione × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9KHI Cryo-EM structure of heteromeric TRPC channel Deposited 2024-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded CA CALCIUM ION × 3 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 3 Y01 CHOLESTEROL HEMISUCCINATE × 3 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9KHJ Cryo-EM structure of homomeric TRPC channel, class 1 Deposited 2024-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.62 Å
9KHK Cryo-EM structure of homomeric TRPC channel, class 2 Deposited 2024-11-10 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9LZY Cryo-EM structure of homomeric TRPC channel with agonists, class 1 Deposited 2025-02-22 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 A1L55 (-)-englerin A × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.43 Å
9LZZ Cryo-EM structure of homomeric TRPC channel with agonists, class 2 Deposited 2025-02-22 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 A1L55 (-)-englerin A × 4 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.03 Å
9M36 Structure of human TRPC5 in the low Ca environment. Deposited 2025-02-28 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–764（764 aa）
Chain B 1–764（764 aa）
Chain C 1–764（764 aa）
Chain D 1–764（764 aa）
Not recorded ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L5I [(2~{S})-2-[(~{E})-octadec-9-enoyl]oxy-3-oxidanyl-propyl] octadec-9-enoate × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.48 Å
9M4W Structure of human TRPC5 bound with (-)-englerin A, class 2. Deposited 2025-03-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–764（764 aa）
Chain B 1–764（764 aa）
Chain C 1–764（764 aa）
Chain D 1–764（764 aa）
Not recorded ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.62 Å
9M5V Structure of human TRPC5 bound with (-)-englerin A,class1 Deposited 2025-03-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–973（973 aa）
Chain B 1–973（973 aa）
Chain C 1–973（973 aa）
Chain D 1–973（973 aa）
Not recorded Y01 CHOLESTEROL HEMISUCCINATE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 A1L55 (-)-englerin A × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.53 Å
9RRF TRPC5 apo cryoEM map in the presence of pluronic acid (PA), state 2 Deposited 2025-06-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 CA CALCIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
9RRM Human TRPC5 in complex with (-) englerin A, full occupancy, state 1, on 290 nm gold foil holes (HexAuFoil) Deposited 2025-06-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded CA CALCIUM ION × 4 ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
9RRN Human TRPC5 in complex with (-) englerin A, full occupancy, state 2, on 290 nm gold foil holes (HexAuFoil) Deposited 2025-06-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
9RRO Human TRPC5 in complex with (-) englerin A, full occupancy, intermediary desensitized state Deposited 2025-06-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded CA CALCIUM ION × 4 ZN ZINC ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9RRQ Human TRPC5 in complex with (-) englerin A, partial occupancy (2EA:2LIP stoichiometry) state 1 Deposited 2025-06-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded CA CALCIUM ION × 4 PTY PHOSPHATIDYLETHANOLAMINE × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 2 ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9RRU Human TRPC5 in complex with (-) englerin A, mixed occupancy, state 1 Deposited 2025-06-30 Parsed fields agree Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9RSH Human TRPC5 in complex with (-) englerin A, mixed occupancy, state 2 Deposited 2025-07-01 Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9RVV Human TRPC5 in complex with (-) englerin A, partial occupancy (2EA:2LIP stoichiometry) state 2 Deposited 2025-07-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–765（765 aa）
Chain B 1–765（765 aa）
Chain C 1–765（765 aa）
Chain D 1–765（765 aa）
Not recorded CA CALCIUM ION × 4 Y01 CHOLESTEROL HEMISUCCINATE × 4 A1L55 (-)-englerin A × 2 ZN ZINC ION × 4 YZY (2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å

29 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TRPC5_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–765； UniProt 1–765 Author chain B； PDBConstruct 1–765； UniProt 1–765 Author chain C； PDBConstruct 1–765； UniProt 1–765 Author chain D； PDBConstruct 1–765； UniProt 1–765

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9rsg

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9rsg
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9rsg
Deposition date deposition_date2025-07-01
Structure title titleHuman TRPC5 in complex with (-) englerin A, mixed occupancy_2, state 2
Keywords keywordsTRPC5, (-) englerin A, agonist, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier43.48 Å
Radius of gyration Rg (electron density) rg_electron42.54 Å
Forward intensity I(0) i0922424000.00
Molecular weight molecular_weight267590.0 kDa
Excluded volume excluded_volume341720 ų
Envelope volume envelope_volume482750 ų
Hydration-shell volume shell_volume89956 ų
Envelope diameter envelope_diameter135.3 Å
Shell Rg shell_rg51.79 Å
Envelope Rg envelope_rg41.35 Å
Shape Rg shape_rg42.53 Å
Total Rg total_rg43.02 Å
Total atoms total_atoms18856
Residues n_residues2313
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax131.5 Å
Rg (real space) rg_real43.15 Å
Rg uncertainty (real space) rg_real_error0.64 Å
I(0) (real space) i0_real9.2240e+08
I(0) uncertainty (real space) i0_real_error1.3850e+07
Rg (reciprocal space) rg_reciprocal43.48 Å
I(0) (reciprocal space) i0_reciprocal922800000.0000
Solution quality estimate total_estimate0.8881
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary58.4 Å
Skewness Skewness skewness0.012
Kurtosis Kurtosis kurtosis-0.480
Angular range angular_range— – 0.1800 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha99380000.0000
Real-space data points n_real_points37
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.902; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.947; Smooth: 0.888
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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8. Citations (1)

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9. Files and Curves (10)