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9s0v

The Crystal Structure of Human Tissue Nonspecific Alkaline Phosphatase (hTNAP) in complex with phosphate

Method: X-RAY DIFFRACTION D_max: 143.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9s0v
deposition_date	2025-07-17
last_revision	2025-09-17
title	The Crystal Structure of Human Tissue Nonspecific Alkaline Phosphatase (hTNAP) in complex with phosphate
keywords	alkaline phosphatase, thermogenesis, structural biology, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	43.31 Å
rg_electron	42.76 Å
i0	1183370000.00
molecular_weight	274150.0 kDa
excluded_volume	338500 Å³
envelope_volume	431690 Å³
shell_volume	81561 Å³
envelope_diameter	161.5 Å
shell_rg	49.67 Å
envelope_rg	42.70 Å
shape_rg	42.76 Å
total_rg	43.06 Å
total_atoms	19221
n_residues	2411
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	143.9 Å
rg_real	43.24 Å
rg_real_error	1.14 Å
i0_real	1.1830e+09
i0_real_error	2.1350e+07
rg_reciprocal	43.31 Å
i0_reciprocal	1183000000.0000
total_estimate	0.8748
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	139.9 Å
skewness	0.314
kurtosis	-0.226
angular_range	— – 0.1800 Å⁻¹
current_alpha	0.0000
highest_alpha	237400000.0000
n_real_points	37
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.853; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.811

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4. Crystallography & Experiment

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5. Entities & Polymer Info (9)

Entity 1 polymer

description	Alkaline phosphatase, tissue-nonspecific isozyme
formula_weight	56454.0 kDa
ec_number	3.1.3.1,3.9.1.1
src_method	man
entity_count	5

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E
atom_count	19230

LVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPAAAAENLYFQGDYKDDDDKHHHHHHHH

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 branched

description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	424.4 kDa
src_method	man
entity_count	10

Entity 3 branched

description	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	586.5 kDa
src_method	man
entity_count	4

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	10

Entity 5 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	5

Entity 6 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	5

Entity 7 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	syn
entity_count	6

Entity 8 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	syn
entity_count	5

Entity 9 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	9

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6. Citations (1)

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length_a	156.730 Å
length_b	297.700 Å
length_c	205.160 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	40

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	3.350 Å
resolution_low	39.560 Å
r_factor	0.2178
r_work	0.2153
r_free	0.2656

fit	/app/data/saxs_computation/s0/9s0v/9s0v.fit
pddf	/app/data/pr_computation/s0/9s0v/9s0v.pr.out
profile	/app/data/saxs_computation/s0/9s0v/9s0v.dat