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9sd5

Crystal structure of the C-terminally truncated human E574Q-PGGHG mutant in complex with glucose

Method: X-RAY DIFFRACTION D_max: 96.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9sd5
deposition_date	2025-08-12
last_revision	2026-02-11
title	Crystal structure of the C-terminally truncated human E574Q-PGGHG mutant in complex with glucose
keywords	Collagen, glycosylhydrolase, PGGHG, SUGAR BINDING PROTEIN; SUGAR BINDING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	26.94 Å
rg_electron	25.99 Å
i0	96488200.00
molecular_weight	77288.0 kDa
excluded_volume	96549 Å³
envelope_volume	110830 Å³
shell_volume	34873 Å³
envelope_diameter	100.9 Å
shell_rg	34.01 Å
envelope_rg	26.49 Å
shape_rg	26.00 Å
total_rg	26.74 Å
total_atoms	5451
n_residues	686
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	96.4 Å
rg_real	26.89 Å
rg_real_error	0.77 Å
i0_real	9.6490e+07
i0_real_error	1.5170e+06
rg_reciprocal	26.91 Å
i0_reciprocal	96490000.0000
total_estimate	0.8444
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	32.7 Å
skewness	0.371
kurtosis	-0.122
angular_range	— – 0.2950 Å⁻¹
current_alpha	0.0000
highest_alpha	29110000.0000
n_real_points	60
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.680; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.951; Smooth: 0.982

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4. Crystallography & Experiment

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5. Entities & Polymer Info (7)

Entity 1 polymer

description	Protein-glucosylgalactosylhydroxylysine glucosidase
formula_weight	76076.6 kDa
ec_number	3.2.1.107
mutation	Deletion of the last 45 C-terminal residues (692-737) and E574Q
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	6508

GMEDAGEDPTTFAAHSLPSDPRLLATVTNAYLGTRVFHDTLHVSGVYNGAGGDTHRAMLPSPLNVRLEAPAGMGEQLTETFALDTNTGSFLHTLEGPRFRASQCIYAHRTLPHVLAFRVSIARLAPGSGPITLLLRSAFSPESPDLDLHQGPDFQGARYLYGHTLTPEQPGGPQQEVHMLWTPAPPDLTLGEGEEARTWDFLTAVGGSQAEAQACLTEALQLQARGALYTAHAQAWAQLWVECGLDVVGPLQLRQALRGSLYYLLSALPQPKAPGYICHGLSPGGLSNGSREECYWGHVFWDQDLWMFPSILMFHPEAARAILEYRIRTLDGALENAQNLGYQGAKFAWESADSGLEVCPEDIYGVQEVHVNGAVVLAFELYYHTTQDLQLFREAGGWDVVRAVAEFWCSRVEWSPREEKYHLRGVMSPDEYHSGVNNSVYTNVLVQNSLRFAAALAQDLGLPIPSQWLAVADKIKVPFDVEQNFHPEFDGYEPGEVVKQADVVLLGYPVPFSLSPDVRRKNLEIYEAVTSPQGPAMTWSMFAVGWMELKDAVRARGLLDRSFANMAEPFKVWTQNADGSGAVNFLTGMGGFLQAVVFGCTGFRVTRAGVTFDPVCLSGISRVSVSGIFYQGNKLNFSFSEDSVTVEVTARAGPWAPHLEAELWPSQSRLSLLPGHKVSFPRSAGRIQMSPPK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21

Entity 2 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	16

Entity 4 non-polymer

description	beta-D-glucopyranose
formula_weight	180.2 kDa
src_method	syn
entity_count	1

Entity 5 non-polymer

description	alpha-D-glucopyranose
formula_weight	180.2 kDa
src_method	syn
entity_count	1

Entity 6 non-polymer

description	DI(HYDROXYETHYL)ETHER
formula_weight	106.1 kDa
src_method	nat
entity_count	1

Entity 7 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	714

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6. Citations (1)

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length_a	54.071 Å
length_b	77.076 Å
length_c	79.888 Å
angle_alpha	90.00 °
angle_beta	98.55 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.363 Å
resolution_low	79.000 Å
r_factor	—
r_work	0.1357
r_free	0.1573

fit	/app/data/saxs_computation/sd/9sd5/9sd5.fit
pddf	/app/data/pr_computation/sd/9sd5/9sd5.pr.out
profile	/app/data/saxs_computation/sd/9sd5/9sd5.dat