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9u5s

The structure of the BfpBG complex in the T4bP system

Method: ELECTRON MICROSCOPY D_max: 197.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9u5s
deposition_date	2025-03-21
title	The structure of the BfpBG complex in the T4bP system
keywords	SECRETIN FAMILY, C17 SYMMETRY, T4bP, PROTEIN TRANSPORT; PROTEIN TRANSPORT
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	76.85 Å
rg_electron	76.47 Å
i0	14617200000.00
molecular_weight	1020100.0 kDa
excluded_volume	1272900 Å³
envelope_volume	2309200 Å³
shell_volume	236840 Å³
envelope_diameter	216.5 Å
shell_rg	90.43 Å
envelope_rg	71.86 Å
shape_rg	76.49 Å
total_rg	76.55 Å
total_atoms	71791
n_residues	9469
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	197.6 Å
rg_real	76.36 Å
rg_real_error	0.51 Å
i0_real	1.4610e+10
i0_real_error	2.4940e+08
rg_reciprocal	78.39 Å
i0_reciprocal	14680000000.0000
total_estimate	0.8461
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	118.6 Å
skewness	-0.138
kurtosis	-0.746
angular_range	— – 0.1000 Å⁻¹
current_alpha	0.0063
highest_alpha	2288000000.0000
n_real_points	21
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.999; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	Outer membrane lipoprotein BfpB
formula_weight	56512.4 kDa
src_method	man
entity_count	17

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P,Q
atom_count	71791

CSGNGFYKDNLGVIDKNILHADTSLLKSKNKEHYKSSDMVSKTDSIYIGNSSFQTYHGEPLPGKLEGVHGIILRSSTPLGFDEVLSMIQDSSGIPIVKHTTKDVISGGVSSKSLAATVAEKMNSATGGKSTDQFDHLLLEVSSEHQLMDVNYQGALSTFLDKVAANYNLYWTYESGRIAFSNEETKRFSISILPGGKYTSKNSISSDSNSSSGSSGSSGSSSSDSGAELKFDSDVDFWKDIENSIKLILGSDGSYSISTSTSSVIVRTSSANMKKINEYINTLNAQLERQVTIDVAIYNVTTTDSSDLAMSLEALLKHNGGVLGSVSTSNFAATSGTPSFTGYLNGNGDSSNQVLLNLLAEKGKVSVVTSASVTTMSGQPVPLKVGNDRTYVSEIGTVLSQSSTSTTASTSTVTSGFLMNLLPQVADDGNILLQYGVTLSELVGSNNGFDQATVNGTVIQLPNVDSTTFVQSSMLRNGNTLVLAGYEKKRNESVDQGVGTTSFKLLGGALNGSASRTVTVICITPRIIDLKASGE

Source

organism	Escherichia coli O127:H6 str. E2348/69
ncbi_taxonomy_id	574521
host_organism	Escherichia coli

Entity 2 polymer

description	Lipoprotein
formula_weight	12656.1 kDa
src_method	man
entity_count	17

Polymer

type	polypeptide(L)
strand_id	R,S,T,U,V,W,X,Y,Z,a,b,c,d,e,f,g,h

ESANKNEKLFSGDTTSVALQASERTIKNAKADWSVIKGQTLYGVLSDWAQQSGWTVLWHANYNYTLNASAVFKDMSLDDAVKKLLESMGNVSPRVFIKFYNGNHVILVTQSPEG

Source

organism	Escherichia coli O127:H6 str. E2348/69
ncbi_taxonomy_id	574521
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.560 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.56 Å
resolution_method	—
num_particles	75445

fit	/app/data/saxs_computation/u5/9u5s/9u5s.fit
pddf	/app/data/pr_computation/u5/9u5s/9u5s.pr.out
profile	/app/data/saxs_computation/u5/9u5s/9u5s.dat

The structure of the BfpBG complex in the T4bP system

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)