← Back to search

9uai

Crystal structure of cyanobacterial CRD of CmpBCD in the presence of nitrate

Method: X-RAY DIFFRACTION D_max: 65.5 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9uai
deposition_date	2025-04-01
last_revision	2026-04-08
title	Crystal structure of cyanobacterial CRD of CmpBCD in the presence of nitrate
keywords	ABC transporter, bicarbonate importer, membrane transporter, nitrate binding, TRANSPORT PROTEIN; TRANSPORT PROTEIN
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	21.13 Å
rg_electron	19.91 Å
i0	29405100.00
molecular_weight	41443.0 kDa
excluded_volume	51699 Å³
envelope_volume	58324 Å³
shell_volume	23755 Å³
envelope_diameter	65.4 Å
shell_rg	27.10 Å
envelope_rg	20.15 Å
shape_rg	19.92 Å
total_rg	20.76 Å
total_atoms	2919
n_residues	373
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	65.5 Å
rg_real	21.00 Å
rg_real_error	0.34 Å
i0_real	2.9410e+07
i0_real_error	3.9130e+05
rg_reciprocal	21.03 Å
i0_reciprocal	29410000.0000
total_estimate	0.9014
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	26.0 Å
skewness	0.181
kurtosis	-0.443
angular_range	— – 0.3750 Å⁻¹
current_alpha	0.0000
highest_alpha	11610000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.911; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.991; Smooth: 0.989

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	Nitrate transport ATP-binding subunits C and D
formula_weight	44472.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E
atom_count	3187

MGSSHHHHHHSSGKHGLEKVNLEIGFVPLTACAPIAVAKEKGFFEKHGLDEVSLVRETSWRGIVDGISGGYLDAAQMPSGLPMWLTLGGAGACKPIVTALTMTRNGNAITLDKHFYDRGIHTLADFKAMLQSTRDRQHRMGVVHPSSMHNLLLRYWLAAGGIDPDLDVELKTIPPAQMVVDLKAGSIDGFCVGEPWNFRAAMENIGFTVATDLEIWNGHPGKVLGVREDWANAYPNTHIALVKALLEACMYCADPNNAEEIRQILSRRAYVSTNVNYIHLGNPDDVTCALDTPMREPAHHQFYGAGVNRPSRSEHLWHITQMARWGDVPFPRNWVEVLEKTCRVGVFSTAARELGLTDITYSRNAIQLFDDIPFNAEDPIGYLNHLDIKRNITMAEIAME

Source

organism	Pseudanabaena sp. Chao 1811
ncbi_taxonomy_id	2963092
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	NITRATE ION
formula_weight	62.0 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	268

▶

6. Citations (1)

▶

length_a	92.271 Å
length_b	49.958 Å
length_c	97.585 Å
angle_alpha	90.00 °
angle_beta	116.70 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	1.750 Å
resolution_low	13.070 Å
r_factor	0.1985
r_work	0.1969
r_free	0.2294

fit	/app/data/saxs_computation/ua/9uai/9uai.fit
pddf	/app/data/pr_computation/ua/9uai/9uai.pr.out
profile	/app/data/saxs_computation/ua/9uai/9uai.dat

Crystal structure of cyanobacterial CRD of CmpBCD in the presence of nitrate

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction