Improved thermostability of a GH10 xylanase based on its X-ray crystal structure

Method: X-RAY DIFFRACTION D_max: 83.0 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uj3
deposition_date	2025-04-16
last_revision	2026-04-22
title	Improved thermostability of a GH10 xylanase based on its X-ray crystal structure
keywords	xylanase, crystal, thermostability, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.89 Å
rg_electron	26.98 Å
i0	100882000.00
molecular_weight	78645.0 kDa
excluded_volume	98401 Å³
envelope_volume	116980 Å³
shell_volume	35558 Å³
envelope_diameter	86.1 Å
shell_rg	34.90 Å
envelope_rg	26.44 Å
shape_rg	26.92 Å
total_rg	27.93 Å
total_atoms	5534
n_residues	706
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	83.0 Å
rg_real	27.74 Å
rg_real_error	0.52 Å
i0_real	1.0090e+08
i0_real_error	1.3560e+06
rg_reciprocal	27.79 Å
i0_reciprocal	100900000.0000
total_estimate	0.9143
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	33.5 Å
skewness	0.152
kurtosis	-0.614
angular_range	— – 0.2850 Å⁻¹
current_alpha	0.0000
highest_alpha	24260000.0000
n_real_points	58
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.978; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.951

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	53.077 Å
length_b	68.492 Å
length_c	90.538 Å
angle_alpha	90.00 °
angle_beta	92.67 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.910 Å
resolution_low	33.660 Å
r_factor	0.1606
r_work	0.1589
r_free	0.1930

description	Beta-xylanase
formula_weight	42407.8 kDa
ec_number	3.2.1.8
src_method	man
entity_count	2

type	polypeptide(L)
strand_id	A,B
atom_count	6212

Improved thermostability of a GH10 xylanase based on its X-ray crystal structure

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Massilia sp. RBM26
ncbi_taxonomy_id	1756047
host_organism	Escherichia coli

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	678

fit	/app/data/saxs_computation/uj/9uj3/9uj3.fit
pddf	/app/data/pr_computation/uj/9uj3/9uj3.pr.out
profile	/app/data/saxs_computation/uj/9uj3/9uj3.dat