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9umz

Cryo-EM structure of the SFTSV NP-RNA complex

Method: ELECTRON MICROSCOPY D_max: 112.8 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9umz
deposition_date	2025-04-23
title	Cryo-EM structure of the SFTSV NP-RNA complex
keywords	SFTSV, N protein, RNP complex, NUCLEAR PROTEIN/RNA, NUCLEAR PROTEIN-RNA complex; NUCLEAR PROTEIN/RNA
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	37.31 Å
rg_electron	37.09 Å
i0	319662000.00
molecular_weight	143220.0 kDa
excluded_volume	178470 Å³
envelope_volume	244920 Å³
shell_volume	52917 Å³
envelope_diameter	114.5 Å
shell_rg	45.67 Å
envelope_rg	35.95 Å
shape_rg	37.14 Å
total_rg	37.43 Å
total_atoms	10018
n_residues	1252
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	112.8 Å
rg_real	37.11 Å
rg_real_error	0.57 Å
i0_real	3.1970e+08
i0_real_error	4.7260e+06
rg_reciprocal	37.24 Å
i0_reciprocal	319700000.0000
total_estimate	0.9075
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	53.7 Å
skewness	0.059
kurtosis	-0.700
angular_range	— – 0.2100 Å⁻¹
current_alpha	0.0000
highest_alpha	162800000.0000
n_real_points	43
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.967; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.896

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	Nucleoprotein
formula_weight	27017.2 kDa
src_method	man
entity_count	5

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E
atom_count	10018

MSEWSRIAVEFGEQQLNLTELEDFARELAYEGLDPALIIKKLKETGGDDWVKDTKFIIVFALTRGNKIVKASGKMSNSGSKRLMALQEKYGLVERAETRLSITPVRVAQSLPTWTCAAAAALKEYLPVGPAVMNLKVENYPPEMMCMAFGSLIPTAGVSEATTKTLMEAYSLWQDAFTKTINVKMRGASKTEVYNSFRDPLHAAVNSVFFPNDVRVKWLKAKGILGPDGVPSRAAEVAAAAYRNL

Source

organism	SFTS virus HB29
ncbi_taxonomy_id	992212
host_organism	Escherichia coli

Entity 2 polymer

description	RNA (28-MER)
formula_weight	8527.7 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	K

UUUUUUUUUUUUUUUUUUUUUUUUUUUU

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.980 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.98 Å
resolution_method	—
num_particles	63930

fit	/app/data/saxs_computation/um/9umz/9umz.fit
pddf	/app/data/pr_computation/um/9umz/9umz.pr.out
profile	/app/data/saxs_computation/um/9umz/9umz.dat

Cryo-EM structure of the SFTSV NP-RNA complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)