Crystal structure of the fusion protein of T-lineage protein Themis and adaptor protein Grb2

Method: X-RAY DIFFRACTION D_max: 128.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uou
deposition_date	2025-04-26
last_revision	2026-04-29
title	Crystal structure of the fusion protein of T-lineage protein Themis and adaptor protein Grb2
keywords	T cell development, Regulatory protein, Signal hub, IMMUNE SYSTEM; IMMUNE SYSTEM
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	37.08 Å
rg_electron	37.36 Å
i0	205891000.00
molecular_weight	119890.0 kDa
excluded_volume	151860 Å³
envelope_volume	208600 Å³
shell_volume	47677 Å³
envelope_diameter	130.1 Å
shell_rg	42.05 Å
envelope_rg	37.07 Å
shape_rg	37.30 Å
total_rg	37.88 Å
total_atoms	8452
n_residues	1043
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	128.9 Å
rg_real	37.20 Å
rg_real_error	1.13 Å
i0_real	2.0590e+08
i0_real_error	3.5730e+06
rg_reciprocal	37.13 Å
i0_reciprocal	205900000.0000
total_estimate	0.8647
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	42.0 Å
skewness	0.436
kurtosis	-0.261
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	31200000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.806; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.918; Smooth: 0.901

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

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6. Citations (1)

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length_a	101.804 Å
length_b	136.544 Å
length_c	116.523 Å
angle_alpha	90.00 °
angle_beta	112.61 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	3.064 Å
resolution_low	35.940 Å
r_factor	0.2641
r_work	0.2611
r_free	0.3021

description	Protein THEMIS,Growth factor receptor-bound protein 2
formula_weight	74246.6 kDa
src_method	man
entity_count	2

type	polypeptide(L)
strand_id	B,A
atom_count	8452

Crystal structure of the fusion protein of T-lineage protein Themis and adaptor protein Grb2

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

fit	/app/data/saxs_computation/uo/9uou/9uou.fit
pddf	/app/data/pr_computation/uo/9uou/9uou.pr.out
profile	/app/data/saxs_computation/uo/9uou/9uou.dat