← Back to search

9uow

Crystal structure of Gossypium hirsutum (Cotton) 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) in open conformation

Method: X-RAY DIFFRACTION D_max: 115.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9uow
deposition_date	2025-04-27
title	Crystal structure of Gossypium hirsutum (Cotton) 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) in open conformation
keywords	Gossypium hirsutum EPSPS Synthase, TRANSFERASE; TRANSFERASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	37.10 Å
rg_electron	36.37 Å
i0	307535000.00
molecular_weight	142330.0 kDa
excluded_volume	178670 Å³
envelope_volume	231500 Å³
shell_volume	52831 Å³
envelope_diameter	123.1 Å
shell_rg	42.67 Å
envelope_rg	35.67 Å
shape_rg	36.37 Å
total_rg	36.79 Å
total_atoms	9958
n_residues	1326
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	115.9 Å
rg_real	36.95 Å
rg_real_error	0.70 Å
i0_real	3.0750e+08
i0_real_error	4.7040e+06
rg_reciprocal	37.05 Å
i0_reciprocal	307600000.0000
total_estimate	0.8971
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	51.2 Å
skewness	0.173
kurtosis	-0.455
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	99070000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.929; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.871

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (6)

Entity 1 polymer

description	3-phosphoshikimate 1-carboxyvinyltransferase
formula_weight	48340.4 kDa
ec_number	2.5.1.19
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,B,C
atom_count	10161

ASTATAEKPSRASEIVLQPINEISGTVKLPGSKSLSNRILLLAALSEGTTVVENLLNSDDVHHMLVALGKLGLYVKHDSEKKQAIVEGCGGQFPVGKGEGQEIELFLGNAGTAMRPLTAAITAAGGNSSYVLDGVPRMRERPIGDLVTGLKQLGADVDCTLGTNCPPVRIEGKGGLPGGKVKLSGSISSQYLTALLMAAPLALGDVEIEIIDKLISIPYVEMTIKLMERFGVTVEHTDSWDRFFIRGGQKYMSPGNAYVEGDASSASYFLAGAAVTGGTVTVEGCGTSSLQGDVKFAEVLEMMGAKVTWTKNSVTVTGPPRNPSGRKHLRAIDVNMNKMPDVAMTLAVVALYADGPTAIRDVASWRVKETERMIAICTELRKLGATVEEGPDFCVITPPEKLNVTAIDTYDDHRMAMAFSLAACAEVPVTIKDPGCTRKTFPDYFEVLARVTKH

Source

organism	Gossypium hirsutum
common_name	cotton
ncbi_taxonomy_id	3635
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	DI(HYDROXYETHYL)ETHER
formula_weight	106.1 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	1,2-ETHANEDIOL
formula_weight	62.1 kDa
src_method	syn
entity_count	5

Entity 4 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	1

Entity 5 non-polymer

description	GLYPHOSATE
formula_weight	170.1 kDa
src_method	syn
entity_count	1

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	203

▶

6. Citations (1)

▶

length_a	79.855 Å
length_b	89.942 Å
length_c	116.233 Å
angle_alpha	90.00 °
angle_beta	107.32 °
angle_gamma	90.00 °
z_pdb	6

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.200 Å
resolution_low	24.470 Å
r_factor	0.2035
r_work	0.2011
r_free	0.2477

fit	/app/data/saxs_computation/uo/9uow/9uow.fit
pddf	/app/data/pr_computation/uo/9uow/9uow.pr.out
profile	/app/data/saxs_computation/uo/9uow/9uow.dat