Cryo-EM structure of the human P2X3 receptor in the camlipixant-bound inhibited state

Method: ELECTRON MICROSCOPY D_max: 117.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9ux4
deposition_date	2025-05-13
title	Cryo-EM structure of the human P2X3 receptor in the camlipixant-bound inhibited state
keywords	P2X ion channal, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	34.71 Å
rg_electron	34.53 Å
i0	372435000.00
molecular_weight	105220.0 kDa
excluded_volume	102670 Å³
envelope_volume	199360 Å³
shell_volume	49118 Å³
envelope_diameter	122.0 Å
shell_rg	39.90 Å
envelope_rg	35.24 Å
shape_rg	34.50 Å
total_rg	34.88 Å
total_atoms	7962
n_residues	984
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	117.7 Å
rg_real	34.83 Å
rg_real_error	1.05 Å
i0_real	3.7240e+08
i0_real_error	6.4190e+06
rg_reciprocal	34.76 Å
i0_reciprocal	372400000.0000
total_estimate	0.8476
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	38.4 Å
skewness	0.552
kurtosis	0.032
angular_range	— – 0.2300 Å⁻¹
current_alpha	0.0000
highest_alpha	42400000.0000
n_real_points	47
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.748; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.771

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	P2X purinoceptor 3
formula_weight	36732.4 kDa
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,B,C
atom_count	7962

SWTIGIINRVVQLLIISYFVGWVFLHEKAYQVRDTAIESSVVTKVKGSGLYANRVMDVSDYVTPPQGTSVFVIITKMIVTENQMQGFCPESEEKYRCVSDSQCGPERLPGGGILTGRCVNYSSVLRTCEIQGWCPTEVDTVETPIMMEAENFTIFIKNSIRFPLFNFEKGNLLPNLTARDMKTCRFHPDKDPFCPILRVGDVVKFAGQDFAKLARTGGVLGIKIGWVCDLDKAWDQCIPKYSFTRLDSVSEKSSVSPGYNFRFAKYYKMENGSEYRTLLKAFGIRFDVLVYGNAGKFNIIPTIISSVAAFTSVGVGTVLCDIILLNFL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 non-polymer

description	Camlipixant
formula_weight	458.5 kDa
src_method	syn
entity_count	3

Entity 3 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	syn
entity_count	9

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6. Citations (1)

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resolution	2.57 Å
resolution_method	—
num_particles	158665

fit	/app/data/saxs_computation/ux/9ux4/9ux4.fit
pddf	/app/data/pr_computation/ux/9ux4/9ux4.pr.out
profile	/app/data/saxs_computation/ux/9ux4/9ux4.dat