Apg mutant enzyme D448N of the human gut flora K. grimontii TD1 acarbose hydrolase

Method: X-RAY DIFFRACTION D_max: 85.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9v0i
deposition_date	2025-05-18
last_revision	2025-09-03
title	Apg mutant enzyme D448N of the human gut flora K. grimontii TD1 acarbose hydrolase
keywords	Hydrolase; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	26.49 Å
rg_electron	25.35 Å
i0	75869800.00
molecular_weight	67681.0 kDa
excluded_volume	84362 Å³
envelope_volume	98612 Å³
shell_volume	31979 Å³
envelope_diameter	88.4 Å
shell_rg	33.16 Å
envelope_rg	25.72 Å
shape_rg	25.35 Å
total_rg	26.16 Å
total_atoms	4795
n_residues	592
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	85.9 Å
rg_real	26.47 Å
rg_real_error	0.59 Å
i0_real	7.5870e+07
i0_real_error	1.0870e+06
rg_reciprocal	26.48 Å
i0_reciprocal	75870000.0000
total_estimate	0.8905
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	30.1 Å
skewness	0.358
kurtosis	-0.294
angular_range	— – 0.3000 Å⁻¹
current_alpha	0.0000
highest_alpha	16940000.0000
n_real_points	61
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.873; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.955

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	75.499 Å
length_b	75.499 Å
length_c	401.388 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	12

space_group	P 65 2 2
int_tables_number	179
crystal_system	hexagonal

resolution	2.300 Å
resolution_low	37.750 Å
r_factor	0.2197
r_work	0.2173
r_free	0.2658

description	Maltodextrin glucosidase
formula_weight	69107.7 kDa
ec_number	3.2.1.135,3.2.1.20
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	4976

Apg mutant enzyme D448N of the human gut flora K. grimontii TD1 acarbose hydrolase

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Klebsiella grimontii
ncbi_taxonomy_id	2058152
host_organism	Escherichia coli BL21(DE3)

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	181

fit	/app/data/saxs_computation/v0/9v0i/9v0i.fit
pddf	/app/data/pr_computation/v0/9v0i/9v0i.pr.out
profile	/app/data/saxs_computation/v0/9v0i/9v0i.dat