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9vix

human alpha 7 nicotinic acetylcholine receptor L278A mutant in complex with L-nicotine (desensitized state)

Method: ELECTRON MICROSCOPY D_max: 153.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9vix
deposition_date	2025-06-19
title	human alpha 7 nicotinic acetylcholine receptor L278A mutant in complex with L-nicotine (desensitized state)
keywords	Ligand-gated ion channel, Nicotine, Desensitization, Recovery, Closed pore, MEMBRANE PROTEIN, mutant; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	44.48 Å
rg_electron	43.97 Å
i0	708781000.00
molecular_weight	229720.0 kDa
excluded_volume	291340 Å³
envelope_volume	383400 Å³
shell_volume	73932 Å³
envelope_diameter	162.3 Å
shell_rg	47.51 Å
envelope_rg	44.07 Å
shape_rg	44.01 Å
total_rg	43.99 Å
total_atoms	16180
n_residues	2000
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	153.3 Å
rg_real	44.78 Å
rg_real_error	1.75 Å
i0_real	7.0880e+08
i0_real_error	1.3240e+07
rg_reciprocal	44.48 Å
i0_reciprocal	708500000.0000
total_estimate	0.8162
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	48.2 Å
skewness	0.603
kurtosis	-0.040
angular_range	— – 0.1750 Å⁻¹
current_alpha	0.0000
highest_alpha	129000000.0000
n_real_points	36
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.655; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.957; Smooth: 0.684

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Neuronal acetylcholine receptor subunit alpha-7
formula_weight	57574.2 kDa
mutation	L278A
src_method	man
entity_count	5

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E
atom_count	16220

MRCSPGGVWLALAASLLHVSLQGEFQRKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLAVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFAGGDYKDDDDK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 branched

description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	424.4 kDa
src_method	man
entity_count	5

Entity 3 non-polymer

description	(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE
formula_weight	162.2 kDa
src_method	syn
entity_count	5

Entity 4 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	syn
entity_count	5

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	5

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.06 Å
resolution_method	—
num_particles	16024

fit	/app/data/saxs_computation/vi/9vix/9vix.fit
pddf	/app/data/pr_computation/vi/9vix/9vix.pr.out
profile	/app/data/saxs_computation/vi/9vix/9vix.dat