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9vkq

Cryo-EM structure of F-ATP synthase from Mycobacteroides abscessus (Rotational State 2)

Method: ELECTRON MICROSCOPY D_max: 166.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9vkq
deposition_date	2025-06-23
title	Cryo-EM structure of F-ATP synthase from Mycobacteroides abscessus (Rotational State 2)
keywords	Hydrolase, ATP synthase, membrane protein, mycobacterium abscessus, mycobacterial, nontuberculous mycobacteria; HYDROLASE
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	49.64 Å
rg_electron	48.94 Å
i0	2548280000.00
molecular_weight	415150.0 kDa
excluded_volume	517530 Å³
envelope_volume	758760 Å³
shell_volume	121930 Å³
envelope_diameter	178.4 Å
shell_rg	58.00 Å
envelope_rg	48.82 Å
shape_rg	48.97 Å
total_rg	49.14 Å
total_atoms	29204
n_residues	3821
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	166.3 Å
rg_real	49.40 Å
rg_real_error	1.15 Å
i0_real	2.5480e+09
i0_real_error	4.5830e+07
rg_reciprocal	49.64 Å
i0_reciprocal	2549000000.0000
total_estimate	0.8535
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	62.4 Å
skewness	0.309
kurtosis	-0.031
angular_range	— – 0.1600 Å⁻¹
current_alpha	0.0000
highest_alpha	606100000.0000
n_real_points	33
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.753; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.976; Smooth: 0.857

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4. Crystallography & Experiment

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5. Entities & Polymer Info (9)

Entity 1 polymer

description	ATP synthase subunit alpha
formula_weight	58915.2 kDa
ec_number	7.1.2.2
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,C,B
atom_count	29204

MTELTISSADISGAIEQYVATFSADPTREEIGIVSDAGDGIAHVEGLPSVMTQELLEFPGGVLGVALNLDERSVGTVVLGDFQDIEEGQQVKRTGEVLSVPVGDAFLGRVINPLGQPIDGRGDIEAETRRALELQAPSVVQRQSVSEPLQTGIKAIDSQTPIGRGQRQLIIGDRKTGKTAVCIDTILNQKQAWETGDPAQQVRCIYVAVGQKGSTVAAVRRTLDEAGALEYTTIVAAPASDAAGFKWLAPYTGSALGQHWMYQGKHVLIVFDDLTKQAEAYRAISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGAGSLTGLPIIETKANDISAYIPTNVISITDGQCFLQTDLFNQGVRPAVNVGVSVSRVGGAAQIKAMKEVAGSLRLELSQYRELEAFAAFASDLDATSKAQLERGARLVELLKQPQNSPYPVEEQVVAIYLGTGGHLDSVPVEDVMRFEQEFLDHVRGSHADILADIRETKKFSEDTETKLTNAVNAFKKSFAATDGSSVEVKGVAAEALDESAVGQETVQVRKPAPKK

Source

organism	Mycobacteroides abscessus subsp. abscessus
ncbi_taxonomy_id	1185650
host_organism	Mycobacteroides abscessus subsp. abscessus

Entity 2 polymer

description	ATP synthase subunit beta
formula_weight	56032.9 kDa
ec_number	7.1.2.2
mutation	FLAG-6xHis tag
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	D,E,F

MTAPTANKTAGRVVRVTGPVVDVEFPRDAVPPLFSALNAEITYEAMAKTLTLEVAQHLGDNLVRTISMQPTDGLVRGVDVVSTGNTIAVPVGDGVKGHVFNALGNCLDEPGYGSDFEKWSIHRKPPAFDQLEPRTEMLETGLKVVDLLTPYVRGGKIALFGGAGVGKTVLIQEMINRIARNFGGTSVFAGVGERTREGNDLWVELADANVLKDTALVFGQMDEPPGTRMRVALSALTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSTLLGRMPSAVGYQPTLADEMGELQERITSTRGRSITSMQAVYVPADDYTDPAPATTFAHLDATTELSRAVFSKGIFPAVDPLASSSTILLPSVVGEEHYRVAQEVIRILQRYQDLQDIIAILGIDELSEEDKQLVGRARRIERFLSQNMMAAEQFTGQPGSTVPLKETIEAFDKLTKGEFDHLPEQAFFLIGGLDDLAKKAESLGAKLGLSGQPPRSPSSGSSGGGGENLYFQDYKDDDDKHHHHHH

Source

organism	Mycobacteroides abscessus subsp. abscessus
ncbi_taxonomy_id	1185650
host_organism	Mycobacteroides abscessus subsp. abscessus

Entity 3 polymer

description	ATP synthase gamma chain
formula_weight	33189.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	G

MGAQIRELRRRIKSAGAIKKITKAQELIATSRIAKAQARVVAARPYATEITNVLTALADDAALDHPLLVERPEPKRAGVLIVSSDRGLCGGYNANVLRVAEELYALLREQGKTPVVYVVGRKALNYYSFRNRKVTEAWTGFSERPEYASAQKIADTLVEAFLAGADDEGDDPGLDGILGVDELHIVYTEFKSMLTQAAVAKRIAPMEVEYVGEAAGPTTQYSFEPDATTLFGALLPRYLATRVYAALLEAAASESASRRRAMKAATDNADELIKGLTLEANGARQAQITQEISEIVGGVNALADAAGH

Source

organism	Mycobacteroides abscessus subsp. abscessus
ncbi_taxonomy_id	1185650
host_organism	Mycobacteroides abscessus subsp. abscessus

Entity 4 polymer

description	ATP synthase epsilon chain
formula_weight	13074.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	H

MSEIDVEIVAVEREIWSGKATFVFTRTTSGEIGILPHHIPLVAQLVDDAAVKIEREGSDDLWWAIDGGFLSITDTKVSILAESAQARADIDEAKAKTDSGSEDPRVAAQGRARLRALGQTV

Source

organism	Mycobacteroides abscessus subsp. abscessus
ncbi_taxonomy_id	1185650
host_organism	Mycobacteroides abscessus subsp. abscessus

Entity 5 polymer

description	ATP synthase subunit b
formula_weight	18543.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	b

MGELHSVASAVTAVAAEAAEEGGKQNNFLIPNGTFFVVLAIFLIVLAVIGTFVVPPIQKVLKAREDMVTKTAEDNRNAAEQFTAAEADYKDELAKARGAATAVRDEARAEGRGILEDMRQRANAEATAVTETAAAELARQGEVTAGELATNVDSLSRTLAERVLGVSLSEPANAGRG

Source

organism	Mycobacteroides abscessus subsp. abscessus
ncbi_taxonomy_id	1185650
host_organism	Mycobacteroides abscessus subsp. abscessus

Entity 6 polymer

description	ATP synthase subunit b-delta
formula_weight	47696.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	d

MSTFIGQLIGFAVIVFLVVKYVVPPVRTLMAKQQDAVRQQLADSKTAADKLVEAEGAHAKAIEDAKADAAQIAEEAKADAVQISKQLREQADAEVERIKVHGQEQIVLQRQQLIRQLRGDLGAESVRRAGDLVRSHVADPSAQSATVDRFLDELSQMAGSVGAAKRPVPGGYSGMHAASRESLAAQVSTFRETAASLDSSALSALAEDIAAVAELLISELVLRKHLSEPVDASENEAKLTLVNSLLGNKIGAPALAIVRSAVTARWSASSDLITSLEYIARLALLERAERDGQIEDVEDQLFRVSRVLDAEPQLATLLSDSTAPAQGRVALLTNVLGGRANEVTTALLAQTVRLLYSVRAEVAVLDVAELAVARRDESVAHVKAAAPITDAQRTRLAQVLGQIYGRTIAVQLDVDPELLGGLVVNIGDEEIDGSLSTRLSAAALHLPN

Source

organism	Mycobacteroides abscessus subsp. abscessus
ncbi_taxonomy_id	1185650
host_organism	Mycobacteroides abscessus subsp. abscessus

Entity 7 non-polymer

description	ADENOSINE-5'-TRIPHOSPHATE
formula_weight	507.2 kDa
src_method	syn
entity_count	4

Entity 8 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	5

Entity 9 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.410 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.41 Å
resolution_method	—
num_particles	13408

fit	/app/data/saxs_computation/vk/9vkq/9vkq.fit
pddf	/app/data/pr_computation/vk/9vkq/9vkq.pr.out
profile	/app/data/saxs_computation/vk/9vkq/9vkq.dat

Cryo-EM structure of F-ATP synthase from Mycobacteroides abscessus (Rotational State 2)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (9)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

Entity 6 polymer

Polymer

Source

Entity 7 non-polymer

Entity 8 non-polymer

Entity 9 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)