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9vvj

Cryo-EM structure of the erlin1/2 complex purified using DDM and GDN

Method: ELECTRON MICROSCOPY D_max: 246.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9vvj
deposition_date	2025-07-15
title	Cryo-EM structure of the erlin1/2 complex purified using DDM and GDN
keywords	Cryo-EM, complex, MEMBRANE PROTEIN, SPFH protein family; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	94.75 Å
rg_electron	94.32 Å
i0	10329400000.00
molecular_weight	901440.0 kDa
excluded_volume	1143800 Å³
envelope_volume	3114900 Å³
shell_volume	283720 Å³
envelope_diameter	236.4 Å
shell_rg	98.51 Å
envelope_rg	83.29 Å
shape_rg	94.37 Å
total_rg	94.17 Å
total_atoms	63388
n_residues	7904
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	246.5 Å
rg_real	93.71 Å
rg_real_error	0.74 Å
i0_real	1.0320e+10
i0_real_error	2.0330e+08
rg_reciprocal	97.91 Å
i0_reciprocal	10440000000.0000
total_estimate	0.8406
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	154.0 Å
skewness	-0.282
kurtosis	-0.770
angular_range	— – 0.0800 Å⁻¹
current_alpha	0.0481
highest_alpha	343100000.0000
n_real_points	17
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.979; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Erlin-1
formula_weight	34461.8 kDa
src_method	man
entity_count	13

Polymer

type	polypeptide(L)
strand_id	A,M,L,K,F,E,D,C,B,J,I,H,G
atom_count	63388

ARVLVAAVVGLVAVLLYASIHKIEEGHLAVYYRGGALLTSPSGPGYHIMLPFITTFRSVQTTLQTDEVKNVPCGTSGGVMIYIDRIEVVNMLAPYAVFDIVRNYTADYDKTLIFNKIHHELNQFCSAHTLQEVYIELFDQIDENLKQALQKDLNLMAPGLTIQAVRVTKPKIPEAIRRNFELMEAEKTKLLIAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLAREKAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVD

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 polymer

description	Erlin-2
formula_weight	34188.5 kDa
src_method	man
entity_count	13

Polymer

type	polypeptide(L)
strand_id	a,m,k,l,f,d,b,c,e,i,g,h,j

LGAVVAVASSFFCASLFSAVHKIEEGHIGVYYRGGALLTSTSGPGFHLMLPFITSYKSVQTTLQTDEVKNVPCGTSGGVMIYFDRIEVVNFLVPNAVYDIVKNYTADYDKALIFNKIHHELNQFCSVHTLQEVYIELFDQIDENLKLALQQDLTSMAPGLVIQAVRVTKPNIPEAIRRNYELMESEKTKLLIAAQKQKVVEKEAETERKKALIEAEKVAQVAEITYGQKVMEKETEKKISEIEDAAFLAREKAKADAECYTAMKIAEANKLKLTPEYLQLMKYKAIASNSKMYFGKDIPNMFMD

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 3 branched

description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	424.4 kDa
src_method	man
entity_count	26

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.600 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.60 Å
resolution_method	—
num_particles	25074

fit	/app/data/saxs_computation/vv/9vvj/9vvj.fit
pddf	/app/data/pr_computation/vv/9vvj/9vvj.pr.out
profile	/app/data/saxs_computation/vv/9vvj/9vvj.dat

Cryo-EM structure of the erlin1/2 complex purified using DDM and GDN

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 branched

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)