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9wsf

Crystal structure of Sigma28/FlgM complex from Pseudomonas aeruginosa at 1.95 Angstrom resolution

Method: X-RAY DIFFRACTION D_max: 76.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9wsf
deposition_date	2025-09-13
last_revision	2026-02-25
title	Crystal structure of Sigma28/FlgM complex from Pseudomonas aeruginosa at 1.95 Angstrom resolution
keywords	P. aeruginosa, FliA/FlgM, Flagella, TRANSCRIPTION; TRANSCRIPTION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.25 Å
rg_electron	20.11 Å
i0	16994700.00
molecular_weight	30078.0 kDa
excluded_volume	37358 Å³
envelope_volume	46139 Å³
shell_volume	19668 Å³
envelope_diameter	77.4 Å
shell_rg	26.14 Å
envelope_rg	20.71 Å
shape_rg	20.10 Å
total_rg	20.98 Å
total_atoms	2118
n_residues	270
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	76.5 Å
rg_real	21.26 Å
rg_real_error	0.53 Å
i0_real	1.6990e+07
i0_real_error	2.2620e+05
rg_reciprocal	21.26 Å
i0_reciprocal	16990000.0000
total_estimate	0.7672
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	24.8 Å
skewness	0.412
kurtosis	-0.051
angular_range	— – 0.3750 Å⁻¹
current_alpha	0.0000
highest_alpha	4416000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.682; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.924; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	RNA polymerase sigma factor FliA
formula_weight	28213.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2304

GPLGSMTAASGVRMYSKAQAQNSQEQLIQRYAPLVKRIAYHLLGRLPASVQVEDLMQAGMIGLLEAAKKYDAGKGASFETYAGIRIRGAMLDEVRKGDWAPRSVHRNTRMVTDAIRAIEARTGRDAKDHEVAAELQLSLEDYYGILSDTQGSRLYSFDDLLQDGEHGLPEDTSLSHNEPIHGLLDERFQAALADAIAKLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRARLADWRSAKL

Source

organism	Pseudomonas aeruginosa
ncbi_taxonomy_id	287
host_organism	Escherichia coli

Entity 2 polymer

description	Negative regulator of flagellin synthesis
formula_weight	11673.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

HMMVIDFNRLNPGSTPATTGRTGSTAAGRPDATGADKAGQAATSAPKSGESVQISETAQNMQKVTDQLQTLPVVDNDKVARIKQAIADGTYQVDSERVASKLLDFESQRAA

Source

organism	Pseudomonas aeruginosa
ncbi_taxonomy_id	287
host_organism	Escherichia coli

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	175

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6. Citations (1)

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length_a	56.028 Å
length_b	139.890 Å
length_c	74.308 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 2 2 21
int_tables_number	20
crystal_system	orthorhombic

resolution	1.950 Å
resolution_low	52.010 Å
r_factor	0.1797
r_work	0.1773
r_free	0.2260

fit	/app/data/saxs_computation/ws/9wsf/9wsf.fit
pddf	/app/data/pr_computation/ws/9wsf/9wsf.pr.out
profile	/app/data/saxs_computation/ws/9wsf/9wsf.dat

Crystal structure of Sigma28/FlgM complex from Pseudomonas aeruginosa at 1.95 Angstrom resolution

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)