SAXSdb — 9x28

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1. Structure Basics

entry_id	9x28
deposition_date	2025-10-04
title	Cryo-EM structure of Borna disease virus RNA polymerase L protein
keywords	Polymerase, Viral protein; VIRAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	39.95 Å
rg_electron	39.37 Å
i0	485820000.00
molecular_weight	185030.0 kDa
excluded_volume	234070 Å³
envelope_volume	317630 Å³
shell_volume	67049 Å³
envelope_diameter	140.0 Å
shell_rg	45.42 Å
envelope_rg	38.71 Å
shape_rg	39.36 Å
total_rg	39.76 Å
total_atoms	13020
n_residues	1657
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	132.3 Å
rg_real	39.92 Å
rg_real_error	1.18 Å
i0_real	4.8580e+08
i0_real_error	8.3790e+06
rg_reciprocal	39.94 Å
i0_reciprocal	485800000.0000
total_estimate	0.8668
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	49.6 Å
skewness	0.381
kurtosis	-0.181
angular_range	— – 0.2000 Å⁻¹
current_alpha	0.0000
highest_alpha	101800000.0000
n_real_points	41
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.813; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.824

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

Entity 1 polymer

description	RNA-directed RNA polymerase L
formula_weight	194970.9 kDa
ec_number	2.7.7.48
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	13020

MSFHASLLREEETPRPVAGINRTDQSLKNPLLGTEVSFCLKSSSLPHHVRALGQIKARNLASCDYYLLFRQVVLPPEVYPIGVLIRAAEAILTVIVSAWKLDHMTKTLYSSVRYALTNPRVRAQLELHIAYQRIVGQVSYSREADIGPKRLGNMSLQFIQSLVIATIDTTSCLMTYNHFLAAADTAKSRCHLLIASVVQGALWEQGSFLDHIINMIDIIDSINLPHDDYFTIIKSIFPYSQGLVMGRHNVSVSSDFASVFAIPELCPQLDSLLKKLLQLDPVLLLMVSSVQKSWYFPEIRMVDGSREQLHKMRVELETPQALLSYGHTLLSIFRAEFIKGYVSKNAKWPPVHLLPGCDKSIKNARELGRWSPAFDRRWQLFEKVVILRIADLDMDPDFNDIVSDKAIISSRRDWVFEYNAAAFWKKYGERLERPPARSGPSRLVNALIDGRLDNIPALLEPFYRGAVEFEDRLTVLVPKEKELKVKGRFFSKQTLAIRIYQVVAEAALKNEVMPYLKTHSMTMSSTALTHLLNRLSHTITKGDSFVINLDYSSWCNGFRPELQAPICRQLDQMFNCGYFFRTGCTLPCFTTFIIQDRFNPPYSLSGEPVEDGVTCAVGTKTMGEGMRQKLWTILTSCWEIIALREINVTFNILGQGDNQTIIIHKSASQNNQLLAERALGALYKHARLAGHNLKVEECWVSDCLYEYGKKLFFRGVPVPGCLKQLSRVTDSTGELFPNLYSKLACLTSSCLSAAMADTSPWVALATGVCLYLIELYVELPPAIIQDESLLTTLCLVGPSIGGLPTPATLPSVFFRGMSDPLPFQLALLQTLIKTTGVTCSLVNRVVKLRIAPYPDWLSLVTDPTSLNIAQVYRPERQIRRWIEEAIATSSHSSRIATFFQQPLTEMAQLLARDLSTMMPLRPRDMSALFALSNVAYGLSIIDLFQKSSTVVSASQAVHIEDVALESVRYKESIIQGLLDTTEGYNMQPYLEGCTYLAAKQLRRLTWGRDLVGVTMPFVAEQFHPHSSVGAKAELYLDAIIYCPQETLRSHHLTTRGDQPLYLGSNTAVKVQRGEITGLAKSRAANLVKDTLVLHQWYKVRKVTDPHLNTLMARFLLEKGYTSDARPSIQGGTLTHRLPSRGDSRQGLTGYVNILSTWLRFSSDYLHSFSKSSDDYTIHFQHVFTYGCLYADSVIRSGGVISTPYLLSASCKTCFEKIDSEEFVLACEPQYRGAEWLISKPVTVPEQITDAEVEFDPCVSASYCLGILIGKSFLVDIRASGHDIMEQRTWANLERFSVSDMQKLPWSIVIRSLWRFLIGARLLQFEKAGLIRMLYAATGPTFSFLMKVFQDSALLMDCAPLDRLSPRINFHSRGDLVAKLVLLPFINPGIVEIEVSGINSKYHAVSEANMDLYIAAAKSVGVKPTQFVEETNDFTARGHHHGCYSLSWSKSRNQSQVLKMVVRKLKLCVLYIYPTVDPAVALDLCHLPALTIILVLGGDPAYYERLLEMDLCGAVSSRVDIPHSLAARTHRGFAVGPDAGPGVIRLDRLESVCYAHPCLEELEFNAYLDSELVDISDMCCLPLATPCKALFRPIYRSLQSFRLALMDNYSFVMDLIMIRGLDIRPHLEEFDELLVVGQHILGQPVLVEVVYYVGVVRKRPVLARHPWSADLKRITVGGRAPCPSAARLRDEDCQGSLLVGLPAGLTQLLIIDWSHPQFEKGSSGGSSGSSGGAWSHPQFEK

Source

organism	Borna disease virus-V
ncbi_taxonomy_id	928296
host_organism	Escherichia coli BL21(DE3)

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6. Citations (1)

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resolution	2.83 Å
resolution_method	—
num_particles	120015

fit	/app/data/saxs_computation/x2/9x28/9x28.fit
pddf	/app/data/pr_computation/x2/9x28/9x28.pr.out
profile	/app/data/saxs_computation/x2/9x28/9x28.dat

Cryo-EM structure of Borna disease virus RNA polymerase L protein

SAXS Profile

P(r) Distribution