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9x47

Cryo-EM structure of nucleotide-free Streptococcus thermophilus FoeAB 1

Method: ELECTRON MICROSCOPY D_max: 127.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9x47
deposition_date	2025-10-10
title	Cryo-EM structure of nucleotide-free Streptococcus thermophilus FoeAB 1
keywords	TRANSPORT PROTEIN; TRANSPORT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	38.11 Å
rg_electron	38.23 Å
i0	226105000.00
molecular_weight	128410.0 kDa
excluded_volume	163680 Å³
envelope_volume	218250 Å³
shell_volume	48884 Å³
envelope_diameter	128.9 Å
shell_rg	43.45 Å
envelope_rg	37.01 Å
shape_rg	38.22 Å
total_rg	38.62 Å
total_atoms	9041
n_residues	1142
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	127.7 Å
rg_real	38.22 Å
rg_real_error	0.89 Å
i0_real	2.2610e+08
i0_real_error	3.0660e+06
rg_reciprocal	38.16 Å
i0_reciprocal	226100000.0000
total_estimate	0.8822
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	43.6 Å
skewness	0.387
kurtosis	-0.410
angular_range	— – 0.2050 Å⁻¹
current_alpha	0.0000
highest_alpha	27920000.0000
n_real_points	42
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.879; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.977; Smooth: 0.850

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	Lipid/multidrug/protein-type ABC exporter, ATP binding/membrane-spanning protein
formula_weight	67879.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	9041

MKALLVYFKGFVKDSILGPVFKLIEASFELLVPMVIAGIVDITIPNRDQNHLFMMIFLLFGLAVIGVVVAISAQYFSSRAAVGYAQALTNDLYAKIMRLSKEKRDEIGTSSLVTRLTGDTFQIQTGINQFLRLFLRAPIIVTGAIIMAFRISPRLTLWFIGMVLVLSLIIVLASRIVNPLYAKIRLINESIVTNTRQQLEGMRVIRAFGQSERELDNFTTVNKELKKWQLKTGVISSLISPLTFLVVNMTLIAILWQGNVQIQGGLLSQGMLIALVNYLLQILAELLKLAMMLTNLNQSFISAKRVQAIFNLQDEDIEAPLIIKKPNQPSIALEIDKMTFTYPTASQPSLQEIDFKTFEGESLGIIGGTGSGKSTLVNLIMNLYKPQFGSLTIFKNQLSPNNLKEWRSWVAYVPQKAELFRGTIRSNLSLGLDYEPSDQELWHALDMAQASDFVREKELGLDEPVESFGRNFSGGQRQRLTIARALMRPAPFLILDDSTSALDYLTESRLLAAIRENLKSTSLIIISQRPNSLKAVDQILVLDKGHQVGLGNHEELLATNKIYQDIHYSQHQKVVADEGGSAWSHPQFEKGGGSGGGSGGSAWSHPQLEK

Source

organism	Streptococcus thermophilus
ncbi_taxonomy_id	1308
host_organism	Escherichia coli

Entity 2 polymer

description	Lipid/multidrug/protein-type ABC exporter, ATP binding/membrane-spanning protein
formula_weight	65864.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MKARNNKSTLKRLCKDILSQCYLFALATVGTIVQVALTVYFPILLGDAVDAVLKMNAVFLMSRLIWQMLLLILANSAIQWLNPLIYNRLIYSYSESLRERVMMKIHAIPFSYLDRQGTGDMVSRVTTDSDQLNNGLLMFFNQFFVGLLTIFVIIITMARLDWFMMLLVLVMTPLSMLVTRFIARKSYQLYRNQTKWRGKQTQLIEESLTQEALVQILNAQDQMVRNFNEVNGKYANYSQGAIFYSSAVNPATRFFNSLLYALITGVGAYRIMSGGTFTVGQLTTFLNYVTQYTKPFNDISSVLSELQSALACADRLYMILDEDEIPETGNAVLDEAEVQGHFQFDHVQFGYLQNKPLIKDLSIDIPAASTVAIVGPTGAGKSTLINLLMRFYDLDKGQILLDGRDITYYTRESLRKQIGMVLQETWLKVATIHDNIAYGNPKASREEVVAAAKAANADFFIKQLPLGYDTFLKDAGQSLSQGQRQLLTIARIFVNVPKILILDEATSSIDTRTELLIQEAFAKLMKGRTSFIIAHRLSTIENADLILVMNNGDIVEHGTHNQLMRTKGMYYRMQTAQKTQNN

Source

organism	Streptococcus thermophilus
ncbi_taxonomy_id	1308
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.20 Å
resolution_method	—
num_particles	47962

fit	/app/data/saxs_computation/x4/9x47/9x47.fit
pddf	/app/data/pr_computation/x4/9x47/9x47.pr.out
profile	/app/data/saxs_computation/x4/9x47/9x47.dat

Cryo-EM structure of nucleotide-free Streptococcus thermophilus FoeAB 1

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)