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9x4a

Cryo-EM structure of Streptococcus thermophilus FoeAB E504Q mutant in complex with ATP

Method: ELECTRON MICROSCOPY D_max: 124.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9x4a
deposition_date	2025-10-10
title	Cryo-EM structure of Streptococcus thermophilus FoeAB E504Q mutant in complex with ATP
keywords	TRANSPORT PROTEIN; TRANSPORT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.96 Å
rg_electron	37.56 Å
i0	233520000.00
molecular_weight	129210.0 kDa
excluded_volume	164200 Å³
envelope_volume	208240 Å³
shell_volume	47919 Å³
envelope_diameter	131.8 Å
shell_rg	41.90 Å
envelope_rg	37.71 Å
shape_rg	37.54 Å
total_rg	37.91 Å
total_atoms	9087
n_residues	1140
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	124.6 Å
rg_real	37.33 Å
rg_real_error	1.05 Å
i0_real	2.3350e+08
i0_real_error	4.2300e+06
rg_reciprocal	37.10 Å
i0_reciprocal	233500000.0000
total_estimate	0.8067
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	34.8 Å
skewness	0.603
kurtosis	-0.228
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	70940000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.710; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.828; Smooth: 0.525

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Lipid/multidrug/protein-type ABC exporter, ATP binding/membrane-spanning protein
formula_weight	67879.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	9087

MKALLVYFKGFVKDSILGPVFKLIEASFELLVPMVIAGIVDITIPNRDQNHLFMMIFLLFGLAVIGVVVAISAQYFSSRAAVGYAQALTNDLYAKIMRLSKEKRDEIGTSSLVTRLTGDTFQIQTGINQFLRLFLRAPIIVTGAIIMAFRISPRLTLWFIGMVLVLSLIIVLASRIVNPLYAKIRLINESIVTNTRQQLEGMRVIRAFGQSERELDNFTTVNKELKKWQLKTGVISSLISPLTFLVVNMTLIAILWQGNVQIQGGLLSQGMLIALVNYLLQILAELLKLAMMLTNLNQSFISAKRVQAIFNLQDEDIEAPLIIKKPNQPSIALEIDKMTFTYPTASQPSLQEIDFKTFEGESLGIIGGTGSGKSTLVNLIMNLYKPQFGSLTIFKNQLSPNNLKEWRSWVAYVPQKAELFRGTIRSNLSLGLDYEPSDQELWHALDMAQASDFVREKELGLDEPVESFGRNFSGGQRQRLTIARALMRPAPFLILDDSTSALDYLTESRLLAAIRENLKSTSLIIISQRPNSLKAVDQILVLDKGHQVGLGNHEELLATNKIYQDIHYSQHQKVVADEGGSAWSHPQFEKGGGSGGGSGGSAWSHPQLEK

Source

organism	Streptococcus thermophilus
ncbi_taxonomy_id	1308
host_organism	Escherichia coli

Entity 2 polymer

description	Lipid/multidrug/protein-type ABC exporter, ATP binding/membrane-spanning protein
formula_weight	65863.1 kDa
mutation	E504Q
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MKARNNKSTLKRLCKDILSQCYLFALATVGTIVQVALTVYFPILLGDAVDAVLKMNAVFLMSRLIWQMLLLILANSAIQWLNPLIYNRLIYSYSESLRERVMMKIHAIPFSYLDRQGTGDMVSRVTTDSDQLNNGLLMFFNQFFVGLLTIFVIIITMARLDWFMMLLVLVMTPLSMLVTRFIARKSYQLYRNQTKWRGKQTQLIEESLTQEALVQILNAQDQMVRNFNEVNGKYANYSQGAIFYSSAVNPATRFFNSLLYALITGVGAYRIMSGGTFTVGQLTTFLNYVTQYTKPFNDISSVLSELQSALACADRLYMILDEDEIPETGNAVLDEAEVQGHFQFDHVQFGYLQNKPLIKDLSIDIPAASTVAIVGPTGAGKSTLINLLMRFYDLDKGQILLDGRDITYYTRESLRKQIGMVLQETWLKVATIHDNIAYGNPKASREEVVAAAKAANADFFIKQLPLGYDTFLKDAGQSLSQGQRQLLTIARIFVNVPKILILDQATSSIDTRTELLIQEAFAKLMKGRTSFIIAHRLSTIENADLILVMNNGDIVEHGTHNQLMRTKGMYYRMQTAQKTQNN

Source

organism	Streptococcus thermophilus
ncbi_taxonomy_id	1308
host_organism	Escherichia coli

Entity 3 non-polymer

description	ADENOSINE-5'-TRIPHOSPHATE
formula_weight	507.2 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.16 Å
resolution_method	—
num_particles	42885

fit	/app/data/saxs_computation/x4/9x4a/9x4a.fit
pddf	/app/data/pr_computation/x4/9x4a/9x4a.pr.out
profile	/app/data/saxs_computation/x4/9x4a/9x4a.dat

Cryo-EM structure of Streptococcus thermophilus FoeAB E504Q mutant in complex with ATP

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)