Crystal structure of Mrt4 (L96C) mutant

Method: X-RAY DIFFRACTION D_max: 79.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9xb4
deposition_date	2025-10-23
last_revision	2026-02-04
title	Crystal structure of Mrt4 (L96C) mutant
keywords	;Fumaramidmycin-based derivatives, Azole-resistant fungi, ribosome assembly factor, target identification, protein-RNA interaction, RNA BINDING PROTEIN ;; RNA BINDING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	23.30 Å
rg_electron	22.93 Å
i0	11374000.00
molecular_weight	24781.0 kDa
excluded_volume	30749 Å³
envelope_volume	38715 Å³
shell_volume	15637 Å³
envelope_diameter	81.0 Å
shell_rg	27.35 Å
envelope_rg	22.93 Å
shape_rg	22.90 Å
total_rg	23.63 Å
total_atoms	1727
n_residues	214
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	79.2 Å
rg_real	23.57 Å
rg_real_error	0.77 Å
i0_real	1.1370e+07
i0_real_error	1.6170e+05
rg_reciprocal	23.51 Å
i0_reciprocal	11370000.0000
total_estimate	0.8041
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	19.5 Å
skewness	0.527
kurtosis	-0.502
angular_range	— – 0.3400 Å⁻¹
current_alpha	0.0000
highest_alpha	2405000.0000
n_real_points	66
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.639; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.579; Smooth: 0.953

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	60.245 Å
length_b	65.541 Å
length_c	71.205 Å
angle_alpha	90.00 °
angle_beta	112.12 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.500 Å
resolution_low	41.560 Å
r_factor	0.2233
r_work	0.2166
r_free	0.2827

description	Large ribosomal subunit protein uL10
formula_weight	31817.8 kDa
mutation	L96C
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	1857

Crystal structure of Mrt4 (L96C) mutant

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
common_name	Thermochaetoides thermophila
ncbi_taxonomy_id	759272
host_organism	Escherichia coli BL21(DE3)

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	76

fit	/app/data/saxs_computation/xb/9xb4/9xb4.fit
pddf	/app/data/pr_computation/xb/9xb4/9xb4.pr.out
profile	/app/data/saxs_computation/xb/9xb4/9xb4.dat