His-tagged Glutamine Synthetase on a Ni-NTA lipid monolayer grid

Method: ELECTRON MICROSCOPY D_max: 161.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9y4a
deposition_date	2025-09-02
title	His-tagged Glutamine Synthetase on a Ni-NTA lipid monolayer grid
keywords	Enzyme, BIOSYNTHETIC PROTEIN; BIOSYNTHETIC PROTEIN
method	ELECTRON MICROSCOPY

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	55.69 Å
rg_electron	55.03 Å
i0	4367250000.00
molecular_weight	565560.0 kDa
excluded_volume	709750 Å³
envelope_volume	981670 Å³
shell_volume	138090 Å³
envelope_diameter	162.5 Å
shell_rg	67.58 Å
envelope_rg	53.05 Å
shape_rg	55.00 Å
total_rg	55.38 Å
total_atoms	39876
n_residues	4968
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	161.4 Å
rg_real	55.24 Å
rg_real_error	1.15 Å
i0_real	4.3670e+09
i0_real_error	8.5240e+07
rg_reciprocal	56.06 Å
i0_reciprocal	4372000000.0000
total_estimate	0.8601
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	74.9 Å
skewness	-0.090
kurtosis	-0.558
angular_range	— – 0.1400 Å⁻¹
current_alpha	0.0000
highest_alpha	287200000.0000
n_real_points	29
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.928; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.939; Smooth: 0.453

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (1)

▶

6. Citations (1)

▶

length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.100 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.10 Å
resolution_method	—
num_particles	96036

description	Glutamine synthetase
formula_weight	53085.9 kDa
ec_number	6.3.1.2
src_method	man
entity_count	12

His-tagged Glutamine Synthetase on a Ni-NTA lipid monolayer grid

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H,I,J,K,L
atom_count	39876

organism	Staphylococcus aureus
ncbi_taxonomy_id	1280
host_organism	Escherichia coli

fit	/app/data/saxs_computation/y4/9y4a/9y4a.fit
pddf	/app/data/pr_computation/y4/9y4a/9y4a.pr.out
profile	/app/data/saxs_computation/y4/9y4a/9y4a.dat