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9yjz

cryoEM structure of Apo Aspergillus fumigatus acetolactate synthase (ALS)

Method: ELECTRON MICROSCOPY D_max: 109.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9yjz
deposition_date	2025-10-06
title	cryoEM structure of Apo Aspergillus fumigatus acetolactate synthase (ALS)
keywords	Branched-Chain Amino Acid Synthesis, antifungal target, herbicide target, anti fugal infection, PLANT PROTEIN; PLANT PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	32.32 Å
rg_electron	31.96 Å
i0	261559000.00
molecular_weight	129960.0 kDa
excluded_volume	162700 Å³
envelope_volume	194220 Å³
shell_volume	49464 Å³
envelope_diameter	117.3 Å
shell_rg	39.94 Å
envelope_rg	32.32 Å
shape_rg	31.97 Å
total_rg	32.48 Å
total_atoms	9216
n_residues	1164
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	109.6 Å
rg_real	32.32 Å
rg_real_error	0.72 Å
i0_real	2.6160e+08
i0_real_error	4.2430e+06
rg_reciprocal	32.32 Å
i0_reciprocal	261600000.0000
total_estimate	0.8684
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	38.0 Å
skewness	0.424
kurtosis	-0.160
angular_range	— – 0.2450 Å⁻¹
current_alpha	0.0000
highest_alpha	240700000.0000
n_real_points	50
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.785; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.934

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Acetolactate synthase
formula_weight	70307.8 kDa
ec_number	2.2.1.6
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	9216

SPAFNQEPHRNEVSPLQNRHLPELDDSTSLITVMSRFVGLSGGQIFHEMMLRLGVKHIFGYPGGAILPVFDAIYNSKHFDFVLPRHEQGAGHMAEGYARASGKPGVVLVTSGPGATNVITPMQDALSDGTPMVVFCGQVPTSSIGTDSFQEADVVGISRACTKWNVMVKSVAELPRRIQEAFEIATSGRPGPVLVDLPKDITAGILRKPIPMNSTIPSLPSAATMAARELSMKQLESSIDRVARLVNIAQKPVLYVGQGLLARPDGPKVLKELADKACIPVTTTLQGLGGFDELDPKALHMLGMHGSAYANMAMQEADLIIAVGARFDDRVTGNLSKFAPQAKLAASEKRGGIVHFEIMPKNINKVVQANEAVEGDCAENIRLLLPHVKPVSERPEWFAQINDWKARFPFSLYEKQSPEGPIKPQALIEKLSDLTAPIKDRTIITTGVGQHQMWAAQHFRWRHPRTMITSGGLGTMGYGLPAAIGAKVARPDCLVIDIDGDASFNMTLTELTTAAQFNIGVKVLLLNNEEQGMVTQWQNLFYEDRYSHTHQKNPDFVPMARSMGVAAERCTKPSEVEEKLKWLIESDGPALLEVFTDRKVPVLPMVPAGSALHEFLVYDEAKEKERKALLRSRTAPEYLHK

Source

organism	Aspergillus fumigatus
ncbi_taxonomy_id	746128
host_organism	Escherichia coli

Entity 2 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	THIAMINE DIPHOSPHATE
formula_weight	425.3 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	FLAVIN-ADENINE DINUCLEOTIDE
formula_weight	785.5 kDa
src_method	syn
entity_count	2

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.760 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.76 Å
resolution_method	—
num_particles	64577

fit	/app/data/saxs_computation/yj/9yjz/9yjz.fit
pddf	/app/data/pr_computation/yj/9yjz/9yjz.pr.out
profile	/app/data/saxs_computation/yj/9yjz/9yjz.dat

cryoEM structure of Apo Aspergillus fumigatus acetolactate synthase (ALS)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D