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9zb7

A DARPin fused to the 1TEL crystallization chaperone via a direct helical fusion

Method: X-RAY DIFFRACTION D_max: 81.6 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9zb7
deposition_date	2025-11-20
last_revision	2025-12-17
title	A DARPin fused to the 1TEL crystallization chaperone via a direct helical fusion
keywords	;Sterile Alpha Motif (SAM) of Human Translocation ETS Leukemia (TEL), protein crystallization chaperone, TELSAM, ETV6, Transcription, 1TEL Crystallization Chaperone, Oncoprotein, DARPin, Designed Ankyrin Repeat Protein ;; TRANSCRIPTION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.93 Å
rg_electron	21.46 Å
i0	12962400.00
molecular_weight	26395.0 kDa
excluded_volume	32639 Å³
envelope_volume	38877 Å³
shell_volume	16571 Å³
envelope_diameter	81.0 Å
shell_rg	26.54 Å
envelope_rg	21.70 Å
shape_rg	21.43 Å
total_rg	22.27 Å
total_atoms	3562
n_residues	238
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	81.6 Å
rg_real	22.17 Å
rg_real_error	0.94 Å
i0_real	1.2960e+07
i0_real_error	1.9660e+05
rg_reciprocal	22.12 Å
i0_reciprocal	12960000.0000
total_estimate	0.7027
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	20.7 Å
skewness	0.562
kurtosis	-0.224
angular_range	— – 0.3600 Å⁻¹
current_alpha	0.0000
highest_alpha	3026000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.569; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.422; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (9)

Entity 1 polymer

description	Transcription factor ETV6,DARPin
formula_weight	27093.3 kDa
mutation	V112E
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3880

MGHHHHHHHHHHSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFEMNGKALLLLTKEDFRYRSPHSGDELYELLQHILLGRDLLEAARAGQDDEVRILMANGADVNATDNDGYTPLHLAASNGHLEIVEVLLKNGADVNASDLTGITPLHAAAATGHLEIVEVLLKHGADVNAYDNDGHTPLHLAAKYGHLEIVEVLLKHGADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	2-OXOGLUTARIC ACID
formula_weight	146.1 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	AMMONIUM ION
formula_weight	18.0 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	2

Entity 5 non-polymer

description	ETHANOL
formula_weight	46.1 kDa
src_method	syn
entity_count	2

Entity 6 non-polymer

description	POTASSIUM ION
formula_weight	39.1 kDa
src_method	syn
entity_count	5

Entity 7 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	5

Entity 8 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	nat
entity_count	4

Entity 9 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	205

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6. Citations (1)

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length_a	83.690 Å
length_b	83.690 Å
length_c	58.491 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 65
int_tables_number	170
crystal_system	hexagonal

resolution	1.240 Å
resolution_low	18.830 Å
r_factor	0.1467
r_work	0.1456
r_free	0.1702

fit	/app/data/saxs_computation/zb/9zb7/9zb7.fit
pddf	/app/data/pr_computation/zb/9zb7/9zb7.pr.out
profile	/app/data/saxs_computation/zb/9zb7/9zb7.dat