SAXSdb — 9zfo

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1. Structure Basics

entry_id	9zfo
deposition_date	2025-12-01
title	Cryo-EM Structure of Human STAT2-USP18-ISG15 Complex
keywords	Deubiquitinating Enzyme, ISGylation, Transcription Factor, SIGNALING PROTEIN; SIGNALING PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	37.37 Å
rg_electron	37.11 Å
i0	128793000.00
molecular_weight	94272.0 kDa
excluded_volume	119310 Å³
envelope_volume	163970 Å³
shell_volume	37765 Å³
envelope_diameter	126.4 Å
shell_rg	41.82 Å
envelope_rg	37.11 Å
shape_rg	37.08 Å
total_rg	37.54 Å
total_atoms	6635
n_residues	827
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	126.5 Å
rg_real	37.66 Å
rg_real_error	0.99 Å
i0_real	1.2880e+08
i0_real_error	1.8440e+06
rg_reciprocal	37.49 Å
i0_reciprocal	128800000.0000
total_estimate	0.8435
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	30.1 Å
skewness	0.426
kurtosis	-0.565
angular_range	— – 0.2100 Å⁻¹
current_alpha	0.0000
highest_alpha	38740000.0000
n_real_points	43
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.790; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.829; Smooth: 0.762

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Ubl carboxyl-terminal hydrolase 18
formula_weight	40401.7 kDa
ec_number	3.4.19.12
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	U
atom_count	6635

LEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNIGQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRPLELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVDCAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGKQVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGGQYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQETAYLLVYMKMEC

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

Entity 2 polymer

description	Signal transducer and activator of transcription 2
formula_weight	82825.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	S

MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYECGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELK

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

Entity 3 polymer

description	Ubiquitin-like protein ISG15
formula_weight	17147.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKSDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.050 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.05 Å
resolution_method	—
num_particles	143496

fit	/app/data/saxs_computation/zf/9zfo/9zfo.fit
pddf	/app/data/pr_computation/zf/9zfo/9zfo.pr.out
profile	/app/data/saxs_computation/zf/9zfo/9zfo.dat

Cryo-EM Structure of Human STAT2-USP18-ISG15 Complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)