Current Protein Identity:B7MIX3
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5TBZ E. Coli RNA Polymerase complexed with NusG Deposited 2016-09-13 | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric(5) Consistent with protein count |
Chain C
1–1342(1342 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION;298 K;0.1 M sodium dihydrogen phosphate pH 6.5, 12% (w/v) PEG 8000
|
Resolution 7.00 Å R-free 0.395 |
| 5TBZ E. Coli RNA Polymerase complexed with NusG Deposited 2016-09-13 | Assembly 2 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric(5) Consistent with protein count |
Chain H
1–1342(1342 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION;298 K;0.1 M sodium dihydrogen phosphate pH 6.5, 12% (w/v) PEG 8000
|
Resolution 7.00 Å R-free 0.395 |
| 5UI8 structure of sigmaN-holoenzyme Deposited 2017-01-13 | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric(6) Consistent with protein count |
Chain I
1–1342(1342 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP
|
Resolution 3.76 Å R-free 0.312 |
| 6AWB Structure of 30S ribosomal subunit and RNA polymerase complex in non-rotated state Deposited 2017-09-05 | Assembly 1 Protein–RNA Heteromer;Protein × 26 PDB declaration: 27-meric(27) Consistent with all polymers |
Chain 03
2–1341(1340 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7;20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions
Cryogen ETHANE;2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
|
Resolution 6.70 Å |
| 6AWC Structure of 30S ribosomal subunit and RNA polymerase complex in rotated state Deposited 2017-09-05 | Assembly 1 Protein–RNA Heteromer;Protein × 26 PDB declaration: 27-meric(27) Consistent with all polymers |
Chain 03
2–1341(1340 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7;20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions
Cryogen ETHANE;2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
|
Resolution 7.90 Å |
| 6AWD Structure of 30S (S1 depleted) ribosomal subunit and RNA polymerase complex Deposited 2017-09-05 | Assembly 1 Protein–RNA Heteromer;Protein × 25 PDB declaration: 26-meric(26) Consistent with all polymers |
Chain 03
2–1341(1340 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7;20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME
cryo-EM vitrification conditions
Cryogen ETHANE;2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.
|
Resolution 8.10 Å |
| 6B6H The cryo-EM structure of a bacterial class I transcription activation complex Deposited 2017-10-02 | Assembly 1 Other combination Heteromer;Protein × 9 PDB declaration: dodecameric(12) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;20 mM TRIS pH 7.5, 50 mM sodium chloride, 0.1mM EDTA, 5 mM MgCl2, 5 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE;3 second blotting
|
Resolution 3.90 Å |
| 6PB4 The E. coli class-II CAP-dependent transcription activation complex with de novo RNA transcript at the state 2 Deposited 2019-06-13 | Assembly 1 Other combination Heteromer;Protein × 8 PDB declaration: undecameric(11) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;blot for 3 seconds before plunging
|
Resolution 4.35 Å |
| 6PB5 The E. coli class-II CAP-dependent transcription activation complex at the state 1 architecture Deposited 2019-06-13 | Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;blot for 3 seconds before plunging
|
Resolution 4.52 Å |
| 6PB6 The E. coli class-II CAP-dependent transcription activation complex at the state 2 Deposited 2019-06-13 | Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;blot for 3 seconds before plunging
|
Resolution 4.29 Å |
| 6PMI Sigm28-transcription initiation complex with specific promoter at the state 1 Deposited 2019-07-02 | Assembly 1 Other combination Heteromer;Protein × 6 PDB declaration: nonameric(9) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;blot for 3 seconds before plunging
|
Resolution 3.86 Å |
| 6PMJ Sigm28-transcription initiation complex with specific promoter at the state 2 Deposited 2019-07-02 | Assembly 1 Other combination Heteromer;Protein × 6 PDB declaration: nonameric(9) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;blot for 3 seconds before plunging
|
Resolution 3.91 Å |
| 6XH7 CueR-TAC without RNA Deposited 2020-06-18 | Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 CU COPPER (II) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6XH8 CueR-transcription activation complex with RNA transcript Deposited 2020-06-18 | Assembly 1 Other combination Heteromer;Protein × 8 PDB declaration: undecameric(11) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 CU COPPER (II) ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6XL5 Cryo-EM structure of EcmrR-RNAP-promoter open complex (EcmrR-RPo) Deposited 2020-06-28 | Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | 1N7 CHAPSO × 5 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 118 TETRAPHENYLANTIMONIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 6XL9 Cryo-EM structure of EcmrR-RNAP-promoter initial transcribing complex with 3-nt RNA transcript (EcmrR-RPitc-3nt) Deposited 2020-06-28 | Assembly 1 Other combination Heteromer;Protein × 7 PDB declaration: decameric(10) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 2 118 TETRAPHENYLANTIMONIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 6XLJ Cryo-EM structure of EcmrR-RNAP-promoter initial transcribing complex with 4-nt RNA transcript (EcmrR-RPitc-4nt) Deposited 2020-06-28 | Assembly 1 Other combination Heteromer;Protein × 8 PDB declaration: undecameric(11) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | 1N7 CHAPSO × 5 118 TETRAPHENYLANTIMONIUM ION × 3 MG MAGNESIUM ION × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 6XLL Cryo-EM structure of E. coli RNAP-promoter initial transcribing complex with 5-nt RNA transcript (RPitc-5nt) Deposited 2020-06-28 | Assembly 1 Other combination Heteromer;Protein × 6 PDB declaration: nonameric(9) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | 1N7 CHAPSO × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 6XLM Cryo-EM structure of E.coli RNAP-DNA elongation complex 1 (RDe1) in EcmrR-dependent transcription Deposited 2020-06-28 | Assembly 1 Other combination Heteromer;Protein × 6 PDB declaration: nonameric(9) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | 1N7 CHAPSO × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6XLN Cryo-EM structure of E. coli RNAP-DNA elongation complex 2 (RDe2) in EcmrR-dependent transcription Deposited 2020-06-28 | Assembly 1 Other combination Heteromer;Protein × 5 PDB declaration: octameric(8) Consistent with all polymers |
Chain C
1–1342(1342 aa)
|
Not recorded | 1N7 CHAPSO × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |