Current Protein Identity:G0S081
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2MVF Structural insight into an essential assembly factor network on the pre-ribosome Deposited 2014-10-02 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
168–261(94 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.4;280 K;Ionic strength (raw mmCIF value) 5;Pressure ambient
NMR sample composition
10 % D2O, 10 mM TRIS, 90 % H2O, 5 mM sodium chloride, 1 uM DSS, 2 mM TCEP, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 8I9T Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Dbp10-1 Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer;Protein × 52 PDB declaration: 55-meric(55) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8I9V Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Dbp10-2 Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer;Protein × 53 PDB declaration: 56-meric(56) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8I9W Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Dbp10-3 Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 52-meric(52) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8I9X Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Ytm1-1 Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer;Protein × 58 PDB declaration: 60-meric(60) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8I9Y Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Ytm1-2 Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer;Protein × 57 PDB declaration: 59-meric(59) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8I9Z Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Spb4 Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer;Protein × 58 PDB declaration: 60-meric(60) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8IA0 Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Puf6 Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer;Protein × 62 PDB declaration: 64-meric(64) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8PV1 Chaetomium thermophilum pre-60S State 6 - pre-5S rotation - L1 intermediate - composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 55 PDB declaration: 59-meric(59) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.56 Å |
| 8PV2 Chaetomium thermophilum pre-60S State 10 - pre-5S rotation with Ytm1-Erb1 Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 57-meric(57) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 8PV3 Chaetomium thermophilum pre-60S State 9 - pre-5S rotation - immature H68/H69 - composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 55 PDB declaration: 59-meric(59) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8PV4 Chaetomium thermophilum pre-60S State 2 - pre-5S rotation with Rix1 complex - composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 62 PDB declaration: 66-meric(66) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8PV5 Chaetomium thermophilum pre-60S State 8 - pre-5S rotation without Foot - composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 53-meric(53) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.86 Å |
| 8PV6 Chaetomium thermophilum pre-60S State 3 - post-5S rotation with Rix1 complex with Foot - composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 60 PDB declaration: 64-meric(64) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.94 Å |
| 8PV7 Chaetomium thermophilum pre-60S State 1 - pre-5S rotation (Arx1/Nog2 state) - Composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 55 PDB declaration: 59-meric(59) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.12 Å |
| 8PV8 Chaetomium thermophilum pre-60S State 4 - post-5S rotation with Rix1 complex without Foot - composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 57 PDB declaration: 60-meric(60) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.91 Å |
| 8PVK Chaetomium thermophilum pre-60S State 5 - pre-5S rotation - L1 inward - composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 56 PDB declaration: 60-meric(60) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.55 Å |
| 8PVL Chaetomium thermophilum pre-60S State 7 - pre-5S rotation lacking Utp30/ITS2 - composite structure Deposited 2023-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 54 PDB declaration: 58-meric(58) Consistent with all polymers |
Chain CK
1–261(261 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.19 Å |