Current Protein Identity：G1TRL5
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 4UJC mammalian 80S HCV-IRES initiation complex with eIF5B POST-like state Deposited 2014-06-18 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain AB
583–1209（627 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20MM TRIS-HCL, 5MM MGCL2, 100MM KCL, 0.2MM SPERMIDINE, 2MM DTT；pH 7.6；20MM TRIS-HCL, 5MM MGCL2, 100MM KCL, 0.2MM SPERMIDINE, 2MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 93, INSTRUMENT- FEI VITROBOT MARK I, METHOD- BLOT FOR 2-4 SECONDS BEFORE PLUNGING,
|
Resolution 9.50 Å |
| 4UJD mammalian 80S HCV-IRES initiation complex with eIF5B PRE-like state Deposited 2014-06-18 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain BB
583–1209（627 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20MM TRIS-HCL, 5MM MGCL2, 100MM KCL, 0.2MM SPERMIDINE, 2MM DTT；pH 7.6；20MM TRIS-HCL, 5MM MGCL2, 100MM KCL, 0.2MM SPERMIDINE, 2MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 93, INSTRUMENT- FEI VITROBOT MARK II, METHOD- BLOT FOR 2-4 SECONDS BEFORE PLUNGING,
|
Resolution 8.90 Å |
| 7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain x
638–1264（627 aa）
|
Not recorded | ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；4 second blot time, force 3
|
Resolution 3.90 Å |
| 7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain x
638–1264（627 aa）
|
Not recorded | ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；4 second blot time, force 3
|
Resolution 3.70 Å |
| 7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain x
638–1264（627 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE；4 second blot time, force 3
|
Resolution 3.70 Å |