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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P00947 New Search
23PDB entries
44Biological assembly
87chain/assembly records
Main Difference Dimensions in This Set
Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1BUQ SOLUTION STRUCTURE OF DELTA-5-3-KETOSTEROID ISOMERASE COMPLEXED WITH THE STEROID 19-NORTESTOSTERONE-HEMISUCCINATE Deposited 1998-09-04 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：Y55F,Y88F Mutation：Y55F,Y88F NTH SUCCINIC ACID MONO-(13-METHYL-3-OXO-2,3,6,7,8,9,10,11,12,13,14,15,16,17-TETRADECAHYDRO-1H-CYCLOPENTA[A]PHENANTHREN-17-YL) ESTER × 2 SOLUTION NMR
NMR measurement conditions pH 7.2；310 K；Ionic strength (raw mmCIF value) 30 mM；Pressure 1
Resolution not provided
1ISK 3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES Deposited 1997-03-12 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；300 K
Resolution not provided
1OCV the F116W mutant structure of ketosteroid isomerase from Comamonas testosteroni Deposited 2003-02-11 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.50
Resolution 2.00 Å R-free 0.282
1OCV the F116W mutant structure of ketosteroid isomerase from Comamonas testosteroni Deposited 2003-02-11 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：YES Mutation：YES No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.50
Resolution 2.00 Å R-free 0.282
1OGZ Crystal Structure Of 5-3-Ketosteroid Isomerase Mutants P39A Complexed With Equilenin From Pseudomonas Testosteroni Deposited 2003-05-20 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Mutation：YES EQU EQUILENIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.00
Resolution 2.30 Å R-free 0.309
1OHP CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH 5ALPHA-ESTRAN-3,17-DIONE Deposited 2003-05-29 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：YES Mutation：YES ESR 5-ALPHA-ESTRAN-3,17-DIONE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；PEG 4K 24%, 0.2M AMMONIUM ACETATE, 0.1M SODIUM ACETATE PH 4.6
Resolution 1.53 Å R-free 0.229
1OHP CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH 5ALPHA-ESTRAN-3,17-DIONE Deposited 2003-05-29 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：YES Mutation：YES ESR 5-ALPHA-ESTRAN-3,17-DIONE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；PEG 4K 24%, 0.2M AMMONIUM ACETATE, 0.1M SODIUM ACETATE PH 4.6
Resolution 1.53 Å R-free 0.229
1OHS CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT Y14F/D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH ANDROSTANEDIONE Deposited 2003-05-30 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：YES Mutation：YES 5SD 5ALPHA-ANDROSTAN-3,17-DIONE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；PEG 4K 20% 0.2M AMMONIUM ACETATE, 0.1M SODIUM ACETATE PH 4.6
Resolution 1.70 Å R-free 0.249
1OHS CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT Y14F/D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH ANDROSTANEDIONE Deposited 2003-05-30 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：YES Mutation：YES 5SD 5ALPHA-ANDROSTAN-3,17-DIONE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；PEG 4K 20% 0.2M AMMONIUM ACETATE, 0.1M SODIUM ACETATE PH 4.6
Resolution 1.70 Å R-free 0.249
1QJG Crystal structure of delta5-3-ketosteroid isomerase from Pseudomonas testosteroni in complex with equilenin Deposited 1999-06-24 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：YES Mutation：YES EQU EQUILENIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；PEG 4000 25%, 0.1M SODIUM ACETATE, PH 4.6, 0.2M AMMONIUM SULFATE
Resolution 2.30 Å R-free 0.271
1QJG Crystal structure of delta5-3-ketosteroid isomerase from Pseudomonas testosteroni in complex with equilenin Deposited 1999-06-24 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：YES Mutation：YES EQU EQUILENIN × 2 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；PEG 4000 25%, 0.1M SODIUM ACETATE, PH 4.6, 0.2M AMMONIUM SULFATE
Resolution 2.30 Å R-free 0.271
1QJG Crystal structure of delta5-3-ketosteroid isomerase from Pseudomonas testosteroni in complex with equilenin Deposited 1999-06-24 Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain E 1–125（125 aa）
Chain F 1–125（125 aa）
Mutation：YES Mutation：YES EQU EQUILENIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；PEG 4000 25%, 0.1M SODIUM ACETATE, PH 4.6, 0.2M AMMONIUM SULFATE
Resolution 2.30 Å R-free 0.271
3M8C Crystal Structure of Ketosteroid Isomerase D99N from Pseudomonas Testosteroni (tKSI) with Equilenin Bound Deposited 2010-03-17 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：D99N Mutation：D99N GOL GLYCEROL × 2 SO4 SULFATE ION × 7 EQU EQUILENIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions sitting drop；pH 7.2；298 K；1.4 M ammonium sulfate, 40 mM potassium phosphate, 1 mM EDTA, 2 mM DTT, pH 7.2, sitting drop, temperature 298K
Resolution 2.10 Å R-free 0.222
3M8C Crystal Structure of Ketosteroid Isomerase D99N from Pseudomonas Testosteroni (tKSI) with Equilenin Bound Deposited 2010-03-17 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：D99N Mutation：D99N SO4 SULFATE ION × 6 EQU EQUILENIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions sitting drop；pH 7.2；298 K；1.4 M ammonium sulfate, 40 mM potassium phosphate, 1 mM EDTA, 2 mM DTT, pH 7.2, sitting drop, temperature 298K
Resolution 2.10 Å R-free 0.222
3MHE Crystal Structure of Ketosteroid Isomerase P39A from Pseudomonas Testosteroni (tKSI) Deposited 2010-04-07 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：P39A Mutation：P39A SO4 SULFATE ION × 7 POL N-PROPANOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；2.0 M ammonium sulfate, 5% propanol, 20 mM potassium phosphate, 1 mM EDTA, 2 mM DTT, pH 7.2, vapor diffusion, sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP
Resolution 1.72 Å R-free 0.233
3MKI Crystal Structure of Ketosteroid Isomerase D38ED99N from Pseudomonas Testosteroni (tKSI) Deposited 2010-04-14 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：D38E, D99N Mutation：D38E, D99N SO4 SULFATE ION × 11 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；1.2 M ammonium sulfate, 20 mM potassium phosphate, 1 mM EDTA, 2 mM DTT, pH 7.2, vapor diffusion, sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP
Resolution 2.00 Å R-free 0.213
3MKI Crystal Structure of Ketosteroid Isomerase D38ED99N from Pseudomonas Testosteroni (tKSI) Deposited 2010-04-14 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：D38E, D99N Mutation：D38E, D99N SO4 SULFATE ION × 10 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；1.2 M ammonium sulfate, 20 mM potassium phosphate, 1 mM EDTA, 2 mM DTT, pH 7.2, vapor diffusion, sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP
Resolution 2.00 Å R-free 0.213
3MKI Crystal Structure of Ketosteroid Isomerase D38ED99N from Pseudomonas Testosteroni (tKSI) Deposited 2010-04-14 Assembly 3 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：D38E, D99N Mutation：D38E, D99N Mutation：D38E, D99N Mutation：D38E, D99N SO4 SULFATE ION × 42 GOL GLYCEROL × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；1.2 M ammonium sulfate, 20 mM potassium phosphate, 1 mM EDTA, 2 mM DTT, pH 7.2, vapor diffusion, sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP
Resolution 2.00 Å R-free 0.213
3MYT Crystal structure of Ketosteroid Isomerase D38HD99N from Pseudomonas testosteroni (tKSI) Deposited 2010-05-11 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain C 1–125（125 aa）
Mutation：D38HD99N Mutation：D38HD99N SO4 SULFATE ION × 6 EQU EQUILENIN × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions sitting drop；pH 7.2；298 K；1.2 M ammonium sulfate, 40 mM potassium phosphate, 1 mM EDTA, 2mM DTT, 1.8mM equilenin, pH 7.2, sitting drop, temperature 298K
Resolution 1.96 Å R-free 0.282
3MYT Crystal structure of Ketosteroid Isomerase D38HD99N from Pseudomonas testosteroni (tKSI) Deposited 2010-05-11 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain B 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：D38HD99N Mutation：D38HD99N SO4 SULFATE ION × 9 EQU EQUILENIN × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions sitting drop；pH 7.2；298 K；1.2 M ammonium sulfate, 40 mM potassium phosphate, 1 mM EDTA, 2mM DTT, 1.8mM equilenin, pH 7.2, sitting drop, temperature 298K
Resolution 1.96 Å R-free 0.282
3MYT Crystal structure of Ketosteroid Isomerase D38HD99N from Pseudomonas testosteroni (tKSI) Deposited 2010-05-11 Assembly 3 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：D38HD99N Mutation：D38HD99N Mutation：D38HD99N Mutation：D38HD99N SO4 SULFATE ION × 30 EQU EQUILENIN × 6 GOL GLYCEROL × 8 X-RAY DIFFRACTION
X-ray crystallization conditions sitting drop；pH 7.2；298 K；1.2 M ammonium sulfate, 40 mM potassium phosphate, 1 mM EDTA, 2mM DTT, 1.8mM equilenin, pH 7.2, sitting drop, temperature 298K
Resolution 1.96 Å R-free 0.282
3MYT Crystal structure of Ketosteroid Isomerase D38HD99N from Pseudomonas testosteroni (tKSI) Deposited 2010-05-11 Assembly 4 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：D38HD99N Mutation：D38HD99N Mutation：D38HD99N Mutation：D38HD99N SO4 SULFATE ION × 15 EQU EQUILENIN × 3 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions sitting drop；pH 7.2；298 K；1.2 M ammonium sulfate, 40 mM potassium phosphate, 1 mM EDTA, 2mM DTT, 1.8mM equilenin, pH 7.2, sitting drop, temperature 298K
Resolution 1.96 Å R-free 0.282
3NBR Crystal Structure of Ketosteroid Isomerase D38NP39GD99N from Pseudomonas Testosteroni (tKSI) with 4-Androstene-3,17-dione Bound Deposited 2010-06-03 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Mutation：D38NP39GD99N SO4 SULFATE ION × 6 ASD 4-ANDROSTENE-3-17-DIONE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；2 M ammonium sulfate, 20 mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, vapor diffusion, sitting drop, temperature 298K
Resolution 1.73 Å R-free 0.265
3NHX Crystal Structure of Ketosteroid Isomerase D99N from Pseudomonas Testosteroni (tKSI) with 4-Androstene-3,17-dione Bound Deposited 2010-06-14 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Mutation：D99N ASD 4-ANDROSTENE-3-17-DIONE × 2 SO4 SULFATE ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；298 K；2 M ammonium sulfate, 100 mM Tris-HCl, 1 mM EDTA, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.59 Å R-free 0.219
3NM2 Crystal Structure of Ketosteroid Isomerase D38EP39GV40GS42G from Pseudomonas Testosteroni (tKSI) Deposited 2010-06-21 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Not recorded SO4 SULFATE ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；2.0 M ammonium sulfate, 20 mM potassium phosphate, 1 mM EDTA, 1 mM DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.89 Å R-free 0.254
3NUV Crystal structure of ketosteroid isomerase D38ND99N from Pseudomonas testosteroni (tKSI) with 4-Androstene-3,17-dione Bound Deposited 2010-07-07 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：D38N, D99N Mutation：D38N, D99N ASD 4-ANDROSTENE-3-17-DIONE × 2 SO4 SULFATE ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；298 K；1.8 M ammonium sulfate, 100 mM Tris-HCl, 1 mM EDTA, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.76 Å R-free 0.198
3NUV Crystal structure of ketosteroid isomerase D38ND99N from Pseudomonas testosteroni (tKSI) with 4-Androstene-3,17-dione Bound Deposited 2010-07-07 Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：D38N, D99N Mutation：D38N, D99N ASD 4-ANDROSTENE-3-17-DIONE × 4 SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；298 K；1.8 M ammonium sulfate, 100 mM Tris-HCl, 1 mM EDTA, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.76 Å R-free 0.198
3NXJ Crystal Structure of Ketosteroid Isomerase D99N from Pseudomonas Testosteroni (tKSI) Deposited 2010-07-13 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：D99N Mutation：D99N SO4 SULFATE ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；2.0 M ammonium sulfate, 100 mM Tris-HCl, 1 mM EDTA, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.97 Å R-free 0.314
3NXJ Crystal Structure of Ketosteroid Isomerase D99N from Pseudomonas Testosteroni (tKSI) Deposited 2010-07-13 Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：D99N Mutation：D99N SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；2.0 M ammonium sulfate, 100 mM Tris-HCl, 1 mM EDTA, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.97 Å R-free 0.314
3OV4 Crystal Structure of Ketosteroid Isomerase P39GV40GS42G from Pseudomonas Testosteroni (tKSI) bound to Equilenin Deposited 2010-09-15 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：P39G V40G S42G Mutation：P39G V40G S42G SO4 SULFATE ION × 7 EQU EQUILENIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；10mg/ml protein, 1.8 M ammonium sulfate, 40mM potassium phosphate , 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.83 Å R-free 0.228
3OV4 Crystal Structure of Ketosteroid Isomerase P39GV40GS42G from Pseudomonas Testosteroni (tKSI) bound to Equilenin Deposited 2010-09-15 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：P39G V40G S42G Mutation：P39G V40G S42G SO4 SULFATE ION × 6 EQU EQUILENIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；10mg/ml protein, 1.8 M ammonium sulfate, 40mM potassium phosphate , 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.83 Å R-free 0.228
3T8U Crystal structure of ketosteroid isomerase Y14AY55FD99A from Pseudomonas testosteroni Deposited 2011-08-01 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：Y14A, Y55F, D99A Mutation：Y14A, Y55F, D99A SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；2.0 M ammonium sulfate, 40 mM potassium phosphate, 1 mM EDTA, 2 mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.50 Å R-free 0.300
3T8U Crystal structure of ketosteroid isomerase Y14AY55FD99A from Pseudomonas testosteroni Deposited 2011-08-01 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：Y14A, Y55F, D99A Mutation：Y14A, Y55F, D99A SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；2.0 M ammonium sulfate, 40 mM potassium phosphate, 1 mM EDTA, 2 mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.50 Å R-free 0.300
3UNL Crystal structure of ketosteroid isomerase F54G from Pseudomonas testosteroni Deposited 2011-11-15 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：F54G Mutation：F54G SO4 SULFATE ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；1.4 M ammonium sulfate, 20 mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.52 Å R-free 0.268
3UNL Crystal structure of ketosteroid isomerase F54G from Pseudomonas testosteroni Deposited 2011-11-15 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain D 1–125（125 aa）
Mutation：F54G Mutation：F54G SO4 SULFATE ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；1.4 M ammonium sulfate, 20 mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.52 Å R-free 0.268
4L7K Crystal Structure of Ketosteroid Isomerase D38E from Pseudomonas Testosteroni (tKSI) Deposited 2013-06-14 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Chain B 1–125（125 aa）
Mutation：D38E Mutation：D38E GOL GLYCEROL × 1 SO4 SULFATE ION × 11 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；5mg/ml protein, 1.6 M ammonium sulfate, 40mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.354
4L7K Crystal Structure of Ketosteroid Isomerase D38E from Pseudomonas Testosteroni (tKSI) Deposited 2013-06-14 Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain C 1–125（125 aa）
Chain J 1–125（125 aa）
Mutation：D38E Mutation：D38E GOL GLYCEROL × 1 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；5mg/ml protein, 1.6 M ammonium sulfate, 40mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.354
4L7K Crystal Structure of Ketosteroid Isomerase D38E from Pseudomonas Testosteroni (tKSI) Deposited 2013-06-14 Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain D 1–125（125 aa）
Chain I 1–125（125 aa）
Mutation：D38E Mutation：D38E GOL GLYCEROL × 1 SO4 SULFATE ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；5mg/ml protein, 1.6 M ammonium sulfate, 40mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.354
4L7K Crystal Structure of Ketosteroid Isomerase D38E from Pseudomonas Testosteroni (tKSI) Deposited 2013-06-14 Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain E 1–125（125 aa）
Chain G 1–125（125 aa）
Mutation：D38E Mutation：D38E GOL GLYCEROL × 1 SO4 SULFATE ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；5mg/ml protein, 1.6 M ammonium sulfate, 40mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.354
4L7K Crystal Structure of Ketosteroid Isomerase D38E from Pseudomonas Testosteroni (tKSI) Deposited 2013-06-14 Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain F 1–125（125 aa）
Chain K 1–125（125 aa）
Mutation：D38E Mutation：D38E GOL GLYCEROL × 1 SO4 SULFATE ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；5mg/ml protein, 1.6 M ammonium sulfate, 40mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.354
4L7K Crystal Structure of Ketosteroid Isomerase D38E from Pseudomonas Testosteroni (tKSI) Deposited 2013-06-14 Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain H 1–125（125 aa）
Chain O 1–125（125 aa）
Mutation：D38E Mutation：D38E SO4 SULFATE ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；298 K；5mg/ml protein, 1.6 M ammonium sulfate, 40mM potassium phosphate, 1 mM EDTA, 2mM DTT, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.10 Å R-free 0.354
5DRE Crystal Structure of Ketosteroid Isomerase D38GP39GD99N mutant from Pseudomonas Testosteroni (tKSI) Deposited 2015-09-15 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Mutation：D38G, P39G, D99N No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；298 K；Potassium Phosphate 40 mM, Ammonium Sulfate 0.6 M
Resolution 2.15 Å R-free 0.268
5UGI Crystal Structure of Ketosteroid Isomerase D38GF54A mutant from Pseudomonas Testosteroni (tKSI) bound to Equilenin Deposited 2017-01-08 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Mutation：D38G, F54A EQU EQUILENIN × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；298 K；40 mM Potassium Phosphate, 1.0 M Ammonium Sulfate, 0.25 M Trimethyl acetate
Resolution 1.80 Å R-free 0.227
8CHO CRYSTAL STRUCTURE OF DELTA5-3-KETOSTEROID ISOMERASE FROM PSEUDOMONAS TESTOSTERONI Deposited 1998-01-06 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 1–125（125 aa）
Not recorded P4C O-ACETALDEHYDYL-HEXAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.30 Å R-free 0.271

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.