Current Protein Identity：P04166
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AWP RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 1997-10-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
13–103（91 aa）
Fragment：WATER SOLUBLE DOMAIN
Chain B
13–103（91 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Mutation：V45L, V61L Mutation：V45L, V61L | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å R-free 0.225 |
| 1B5M RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 1996-11-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
19–102（84 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
vapor diffusion with slow evaporation；pH 6.5；CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION TOGETHER WITH SLOW EVAPORATION USING 20% PEG 8000 IN 0.1M SODIUM CACODYLATE (PH = 6.5 WITH 0.2 M MAGNESIUM ACETATE., vapor diffusion with slow evaporation
|
Resolution 2.70 Å |
| 1EUE RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 2000-04-19 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
6–91（86 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Mutation：V45I, V61I | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；20% PEG 8000, 0.2 M magnesium acetate, 0.1 M PIPES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 1.80 Å R-free 0.232 |
| 1EUE RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 2000-04-19 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
6–91（86 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Mutation：V45I, V61I | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；20% PEG 8000, 0.2 M magnesium acetate, 0.1 M PIPES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 1.80 Å R-free 0.232 |
| 1ICC RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 2001-03-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
17–103（87 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Mutation：A18S, I32L, L47R | MG MAGNESIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000 Magnesium Acetate PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.240 |
| 1ICC RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 2001-03-30 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
17–103（87 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Mutation：A18S, I32L, L47R | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000 Magnesium Acetate PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.240 |
| 1ICC RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 2001-03-30 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
17–103（87 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Mutation：A18S, I32L, L47R | MG MAGNESIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000 Magnesium Acetate PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.240 |
| 1ICC RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 2001-03-30 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
17–103（87 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Mutation：A18S, I32L, L47R | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000 Magnesium Acetate PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.240 |
| 1ICC RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5 Deposited 2001-03-30 | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
17–103（87 aa）
Fragment：WATER SOLUBLE DOMAIN
Chain C
17–103（87 aa）
Fragment：WATER SOLUBLE DOMAIN
|
Mutation：A18S, I32L, L47R Mutation：A18S, I32L, L47R | MG MAGNESIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000 Magnesium Acetate PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.240 |
| 1LJ0 Structure of quintuple mutant of the rat outer mitocondrial cytochrome b5. Deposited 2002-04-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
12–103（92 aa）
Fragment：water soluble domain
Chain B
12–103（92 aa）
Fragment：water soluble domain
|
Mutation：A18S, I25L, I32L, L47R, L71S Mutation：A18S, I25L, I32L, L47R, L71S | MG MAGNESIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000, MG ACETATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.251 |
| 1LJ0 Structure of quintuple mutant of the rat outer mitocondrial cytochrome b5. Deposited 2002-04-18 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
12–103（92 aa）
Fragment：water soluble domain
Chain C
12–103（92 aa）
Fragment：water soluble domain
|
Mutation：A18S, I25L, I32L, L47R, L71S Mutation：A18S, I25L, I32L, L47R, L71S | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000, MG ACETATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.251 |
| 1LJ0 Structure of quintuple mutant of the rat outer mitocondrial cytochrome b5. Deposited 2002-04-18 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
12–103（92 aa）
Fragment：water soluble domain
Chain D
12–103（92 aa）
Fragment：water soluble domain
|
Mutation：A18S, I25L, I32L, L47R, L71S Mutation：A18S, I25L, I32L, L47R, L71S | MG MAGNESIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000, MG ACETATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.251 |
| 1LJ0 Structure of quintuple mutant of the rat outer mitocondrial cytochrome b5. Deposited 2002-04-18 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
12–103（92 aa）
Fragment：water soluble domain
|
Mutation：A18S, I25L, I32L, L47R, L71S | MG MAGNESIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000, MG ACETATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.251 |
| 1LJ0 Structure of quintuple mutant of the rat outer mitocondrial cytochrome b5. Deposited 2002-04-18 | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
12–103（92 aa）
Fragment：water soluble domain
Chain C
12–103（92 aa）
Fragment：water soluble domain
|
Mutation：A18S, I25L, I32L, L47R, L71S Mutation：A18S, I25L, I32L, L47R, L71S | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000, MG ACETATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.251 |
| 1LJ0 Structure of quintuple mutant of the rat outer mitocondrial cytochrome b5. Deposited 2002-04-18 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
12–103（92 aa）
Fragment：water soluble domain
|
Mutation：A18S, I25L, I32L, L47R, L71S | MG MAGNESIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；278 K；PEG 8000, MG ACETATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.251 |
| 2I89 Structure of septuple mutant of Rat Outer Mitochondrial Membrane Cytochrome B5 Deposited 2006-09-01 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
12–103（92 aa）
Fragment：Water Soluble Domain
|
Mutation：R15H/A18S/E20S/I25L/I32L/L47R/L71S | MG MAGNESIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；278 K；PEG8K 30%, O.2M MgAc, 0.1M Pipes, VAPOR DIFFUSION, HANGING DROP, temperature 278K, pH 6.80
|
Resolution 2.10 Å R-free 0.278 |
| 2I89 Structure of septuple mutant of Rat Outer Mitochondrial Membrane Cytochrome B5 Deposited 2006-09-01 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
12–103（92 aa）
Fragment：Water Soluble Domain
|
Mutation：R15H/A18S/E20S/I25L/I32L/L47R/L71S | MG MAGNESIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；278 K；PEG8K 30%, O.2M MgAc, 0.1M Pipes, VAPOR DIFFUSION, HANGING DROP, temperature 278K, pH 6.80
|
Resolution 2.10 Å R-free 0.278 |
| 2I89 Structure of septuple mutant of Rat Outer Mitochondrial Membrane Cytochrome B5 Deposited 2006-09-01 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
12–103（92 aa）
Fragment：Water Soluble Domain
|
Mutation：R15H/A18S/E20S/I25L/I32L/L47R/L71S | MG MAGNESIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；278 K；PEG8K 30%, O.2M MgAc, 0.1M Pipes, VAPOR DIFFUSION, HANGING DROP, temperature 278K, pH 6.80
|
Resolution 2.10 Å R-free 0.278 |
| 2I89 Structure of septuple mutant of Rat Outer Mitochondrial Membrane Cytochrome B5 Deposited 2006-09-01 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
12–103（92 aa）
Fragment：Water Soluble Domain
|
Mutation：R15H/A18S/E20S/I25L/I32L/L47R/L71S | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；278 K；PEG8K 30%, O.2M MgAc, 0.1M Pipes, VAPOR DIFFUSION, HANGING DROP, temperature 278K, pH 6.80
|
Resolution 2.10 Å R-free 0.278 |
| 2I89 Structure of septuple mutant of Rat Outer Mitochondrial Membrane Cytochrome B5 Deposited 2006-09-01 | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
12–103（92 aa）
Fragment：Water Soluble Domain
Chain C
12–103（92 aa）
Fragment：Water Soluble Domain
|
Mutation：R15H/A18S/E20S/I25L/I32L/L47R/L71S Mutation：R15H/A18S/E20S/I25L/I32L/L47R/L71S | MG MAGNESIUM ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；278 K；PEG8K 30%, O.2M MgAc, 0.1M Pipes, VAPOR DIFFUSION, HANGING DROP, temperature 278K, pH 6.80
|
Resolution 2.10 Å R-free 0.278 |
| 3MUS 2A Resolution Structure of Rat Type B Cytochrome b5 Deposited 2010-05-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
17–102（86 aa）
Fragment：UNP residues 17-102
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；277 K；30%PEG8K, 0.2M MgAc, 0.1M Hepes, pH 6.8, EVAPORATION, temperature 277K
|
Resolution 2.00 Å R-free 0.223 |
| 3MUS 2A Resolution Structure of Rat Type B Cytochrome b5 Deposited 2010-05-03 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
17–102（86 aa）
Fragment：UNP residues 17-102
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；277 K；30%PEG8K, 0.2M MgAc, 0.1M Hepes, pH 6.8, EVAPORATION, temperature 277K
|
Resolution 2.00 Å R-free 0.223 |
| 4HIL 1.25A Resolution Structure of Rat Type B Cytochrome b5 Deposited 2012-10-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
17–102（86 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；293 K；30% (w/v) PEG 8000, 100 sodium cacodylate, 200 mM sodium acetate, pH 6.5, vapor diffusion, temperature 293K
|
Resolution 1.25 Å R-free 0.152 |
| 4HIL 1.25A Resolution Structure of Rat Type B Cytochrome b5 Deposited 2012-10-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
17–102（86 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；293 K；30% (w/v) PEG 8000, 100 sodium cacodylate, 200 mM sodium acetate, pH 6.5, vapor diffusion, temperature 293K
|
Resolution 1.25 Å R-free 0.152 |