Current Protein Identity：P04745
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1C8Q STRUCTURE SOLUTION AND REFINEMENT OF THE RECOMBINANT HUMAN SALIVARY AMYLASE Deposited 2000-06-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
16–511（496 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；273 K；10 mM Tris- HCL containing 5 mM CaCl2, 44% MPD, protein concentration 20 mg/ml, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K
|
Resolution 2.30 Å R-free 0.216 |
| 1JXJ Role of mobile loop in the mechanism of human salivary amylase Deposited 2001-09-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
16–511（496 aa）
|
Mutation：W58L Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；MPD, calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, temperature 298.0K
|
Resolution 1.99 Å R-free 0.198 |
| 1JXK Role of ethe mobile loop in the mehanism of human salivary amylase Deposited 2001-09-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
16–511（496 aa）
Fragment：lacking the loop residues 306-310
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；MPD, calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP at 298K
|
Resolution 1.90 Å R-free 0.200 |
| 1MFU Probing the role of a mobile loop in human salivary amylase: Structural studies on the loop-deleted mutant Deposited 2002-08-13 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
17–511（495 aa）
|
Mutation：deletion of residues 306 through 310 Non-standard monomer：Yes (specific site not provided by mmCIF) | HMC 5-HYDROXYMETHYL-CHONDURITOL × 4 GLC alpha-D-glucopyranose × 1 CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
soaking with acarbose at 1 mM concentration for 24 hours；pH 9；298 K；40% mpd, pH 9.0, soaking with acarbose at 1 mM concentration for 24 hours, temperature 298K
|
Resolution 2.00 Å R-free 0.201 |
| 1MFV Probing the role of a mobile loop in human slaivary amylase: Structural studies on the loop-deleted enzyme Deposited 2002-08-13 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
17–511（495 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | HMC 5-HYDROXYMETHYL-CHONDURITOL × 2 CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
vapordiffusion, combined with soaking with inhibitor at 1 mM concentration；pH 9；298 K；40% MPD, pH 9.0, vapordiffusion, combined with soaking with inhibitor at 1 mM concentration, temperature 298K
|
Resolution 2.00 Å R-free 0.195 |
| 1NM9 Crystal structure of recombinant human salivary amylase mutant W58A Deposited 2003-01-09 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
16–511（496 aa）
|
Mutation：W58A Non-standard monomer：Yes (specific site not provided by mmCIF) | HMC 5-HYDROXYMETHYL-CHONDURITOL × 1 GLC alpha-D-glucopyranose × 1 CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；323 K；MPD, Calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 323K
|
Resolution 2.10 Å R-free 0.196 |
| 1Q4N Structural studies of Phe256Trp of human salivary alpha-amylase: implications for the role of a conserved water molecule and its associated chain in enzyme activity Deposited 2003-08-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
16–511（496 aa）
|
Mutation：F256W Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 TAM TRIS(HYDROXYETHYL)AMINOMETHANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；MPD, Calcium Chloride, Tris, pH 9.00, VAPOR DIFFUSION, HANGING DROP, temperature 100K
|
Resolution 2.07 Å R-free 0.206 |
| 1SMD HUMAN SALIVARY AMYLASE Deposited 1996-01-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
17–511（495 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.60 Å |
| 1XV8 Crystal Structure of Human Salivary Alpha-Amylase Dimer Deposited 2004-10-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
16–511（496 aa）
Fragment：HSA
Chain B
16–511（496 aa）
Fragment：HSA
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；0.2 M Ca acetate, 0.1 M Na cacodylate (pH 6.5), 18% PEG 8K, VAPOR DIFFUSION, HANGING DROP, temperature 100K
|
Resolution 3.00 Å R-free 0.271 |
| 1Z32 Structure-function relationships in human salivary alpha-amylase: Role of aromatic residues Deposited 2005-03-10 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
16–511（496 aa）
|
Mutation：Y151M Non-standard monomer：Yes (specific site not provided by mmCIF) | GLC alpha-D-glucopyranose × 1 AGL 4-amino-4,6-dideoxy-alpha-D-glucopyranose × 1 HMC 5-HYDROXYMETHYL-CHONDURITOL × 1 CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；MPD 40%, pH 9.0, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.60 Å R-free 0.192 |
| 3BLK Role of aromatic residues in starch binding Deposited 2007-12-11 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
16–511（496 aa）
|
Mutation：W316A Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 HMC 5-HYDROXYMETHYL-CHONDURITOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；45% MPD, 0.1M Tris.HCl, 10 mM calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.215 |
| 3BLP Role of aromatic residues in human salivary alpha-amylase Deposited 2007-12-11 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain X
16–511（496 aa）
|
Mutation：W388A Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 HMC 5-HYDROXYMETHYL-CHONDURITOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；45% MPD, 0.1M Tris.HCl, 10 mM calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.205 |
| 3DHP Probing the role of aromatic residues at the secondary saccharide binding sites of human salivary alpha-amylase in substrate hydrolysis and bacterial binding Deposited 2008-06-18 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
16–511（496 aa）
|
Mutation：W134A,W203A,Y276A,W284A,W316A,W388A Non-standard monomer：Yes (specific site not provided by mmCIF) | GLC alpha-D-glucopyranose × 1 CA CALCIUM ION × 1 CL CHLORIDE ION × 1 HMC 5-HYDROXYMETHYL-CHONDURITOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
hanging drop；pH 9；298 K；MPD, pH 9.0, hanging drop, temperature 298K
|
Resolution 1.50 Å R-free 0.186 |