当前蛋白身份：P06621
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1CG2 CARBOXYPEPTIDASE G2 提交 1996-12-20 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
23–415（393 aa）
链 B
23–415（393 aa）
链 C
23–415（393 aa）
链 D
23–415（393 aa）
|
未记录 | ZN ZINC ION × 13 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；291 K；HANGING DROPS WERE FORMED BY MIXING 4 MICROLITERS OF PROTEIN SOLUTION AT 16-20 MG/ML WITH 4 MICROLITERS OF RESERVOIR SOLUTION CONTAINING 10-12% PEG 4000, 0.2M TRIS (PH 7.2), 0.2M ZINC ACETATE 10% GLYCEROL. CRYSTALS GREW AT 18-20 DEGREES WITHIN A FEW DAYS., vapor diffusion - hanging drop, temperature 291K
|
分辨率 2.50 Å R-free 0.224 |
| 6XJ5 Carboxypeptidase G2 modified with a versatile bioconjugate for metalloprotein design 提交 2020-06-22 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
24–415（392 aa）
链 B
24–415（392 aa）
|
突变：S203C 突变：S203C 非标准单体：是（mmCIF未提供具体位点） | ZN ZINC ION × 12 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Reservoir consisted of 750 uL of 0.2 M Tris pH 7.5, 10% PEG 3350, and 5% glycerol. 2 uL of reservoir solution was mixed with 2 uL of protein solution, consisting of 4.7 mg/mL CPG2 in 50 mM Tris 100 mM NaCl pH 7.4 supplemented with 0.1 M ZnSO4
|
分辨率 3.11 Å R-free 0.309 |
| 6XJ5 Carboxypeptidase G2 modified with a versatile bioconjugate for metalloprotein design 提交 2020-06-22 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
24–415（392 aa）
链 D
24–415（392 aa）
|
突变：S203C 突变：S203C 非标准单体：是（mmCIF未提供具体位点） | ZN ZINC ION × 14 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Reservoir consisted of 750 uL of 0.2 M Tris pH 7.5, 10% PEG 3350, and 5% glycerol. 2 uL of reservoir solution was mixed with 2 uL of protein solution, consisting of 4.7 mg/mL CPG2 in 50 mM Tris 100 mM NaCl pH 7.4 supplemented with 0.1 M ZnSO4
|
分辨率 3.11 Å R-free 0.309 |
| 6XJ5 Carboxypeptidase G2 modified with a versatile bioconjugate for metalloprotein design 提交 2020-06-22 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
24–415（392 aa）
链 F
24–415（392 aa）
|
突变：S203C 突变：S203C 非标准单体：是（mmCIF未提供具体位点） | ZN ZINC ION × 17 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Reservoir consisted of 750 uL of 0.2 M Tris pH 7.5, 10% PEG 3350, and 5% glycerol. 2 uL of reservoir solution was mixed with 2 uL of protein solution, consisting of 4.7 mg/mL CPG2 in 50 mM Tris 100 mM NaCl pH 7.4 supplemented with 0.1 M ZnSO4
|
分辨率 3.11 Å R-free 0.309 |
| 6XJ5 Carboxypeptidase G2 modified with a versatile bioconjugate for metalloprotein design 提交 2020-06-22 | Assembly 4 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 G
24–415（392 aa）
链 H
24–415（392 aa）
|
突变：S203C 突变：S203C 非标准单体：是（mmCIF未提供具体位点） | ZN ZINC ION × 13 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Reservoir consisted of 750 uL of 0.2 M Tris pH 7.5, 10% PEG 3350, and 5% glycerol. 2 uL of reservoir solution was mixed with 2 uL of protein solution, consisting of 4.7 mg/mL CPG2 in 50 mM Tris 100 mM NaCl pH 7.4 supplemented with 0.1 M ZnSO4
|
分辨率 3.11 Å R-free 0.309 |
| 7M6U Crystal structure of a circular permutation and computationally designed pro-enzyme of carboxypeptidase G2 提交 2021-03-26 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
89–415（327 aa）
链 A
25–88（64 aa）
链 C
89–415（327 aa）
链 C
25–88（64 aa）
|
突变：K177A 突变：K177A 突变：K177A 突变：K177A | ZN ZINC ION × 11 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；Protein was prepared to a concentration of 19 mg/mL in 50 mM Tris 100 mM NaCl pH 7.4 0.2 mM ZnSO4. Reservoirs containing 750 uL of 20 mM Tris pH 8.0, 10% glycerol, and 10% PEG 3350 were prepared in 24 well hanging drop vapor diffusion plates. Equal volumes of protein solution and reservoir solution were mixed on a cover slip and suspended over the reservoir
|
分辨率 2.59 Å R-free 0.279 |
| 7M6U Crystal structure of a circular permutation and computationally designed pro-enzyme of carboxypeptidase G2 提交 2021-03-26 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
89–415（327 aa）
链 B
25–88（64 aa）
链 D
89–415（327 aa）
链 D
25–88（64 aa）
|
突变：K177A 突变：K177A 突变：K177A 突变：K177A | ZN ZINC ION × 9 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；Protein was prepared to a concentration of 19 mg/mL in 50 mM Tris 100 mM NaCl pH 7.4 0.2 mM ZnSO4. Reservoirs containing 750 uL of 20 mM Tris pH 8.0, 10% glycerol, and 10% PEG 3350 were prepared in 24 well hanging drop vapor diffusion plates. Equal volumes of protein solution and reservoir solution were mixed on a cover slip and suspended over the reservoir
|
分辨率 2.59 Å R-free 0.279 |