当前蛋白身份：P07906
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1C21 E. COLI METHIONINE AMINOPEPTIDASE: METHIONINE COMPLEX 提交 1999-07-22 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–264（263 aa）
片段：METHIONINE
|
突变：R175Q | CO COBALT (II) ION × 2 NA SODIUM ION × 1 MET METHIONINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；K2SO4, NaCl, methionine, N-octanoyl sucrose, PEG 4000, HEPES, CoCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.80 Å |
| 1C22 E. COLI METHIONINE AMINOPEPTIDASE: TRIFLUOROMETHIONINE COMPLEX 提交 1999-07-22 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–264（263 aa）
|
突变：R175Q | CO COBALT (II) ION × 2 NA SODIUM ION × 1 MF3 2-AMINO-4-TRIFLUOROMETHYLSULFANYL-BUTYRIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；HEPES, CoCl2, K2SO4, Methionine, PEG4000, NaCl, N-octanoyl sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.75 Å |
| 1C23 E. COLI METHIONINE AMINOPEPTIDASE: METHIONINE PHOSPHONATE COMPLEX 提交 1999-07-22 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–264（263 aa）
|
突变：R175Q | CO COBALT (II) ION × 2 NA SODIUM ION × 1 MPH (1-AMINO-3-METHYLSULFANYL-PROPYL)-PHOSPHONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；Hepes, NaCl, K2SO4, CoCl2, PEG 4000, methionine, N-octanoyl sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 2.00 Å |
| 1C24 E. COLI METHIONINE AMINOPEPTIDASE: METHIONINE PHOSPHINATE COMPLEX 提交 1999-07-22 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–264（263 aa）
片段：METHIONINE PHOSPHINATE
|
突变：R175Q | CO COBALT (II) ION × 2 NA SODIUM ION × 1 MPJ (1-AMINO-3-METHYLSULFANYL-PROPYL)-PHOSPHINIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；Hepes, NaCl, CoCl2, K2SO4, PEG 4000, N-octanoyl sucrose, methionine, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.70 Å |
| 1C27 E. COLI METHIONINE AMINOPEPTIDASE:NORLEUCINE PHOSPHONATE COMPLEX 提交 1999-07-22 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–264（263 aa）
片段：NORLEUCINE PHOSPHONATE
|
突变：R175Q | CO COBALT (II) ION × 2 NA SODIUM ION × 1 NLP (1-AMINO-PENTYL)-PHOSPHONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；Hepes, NaCl, CoCl2, K2SO4, methionine, N-octanoyl sucrose, PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.95 Å |
| 1MAT STRUCTURE OF THE COBALT-DEPENDENT METHIONINE AMINOPEPTIDASE FROM ESCHERICHIA COLI: A NEW TYPE OF PROTEOLYTIC ENZYME 提交 1992-12-02 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–264（264 aa）
|
未记录 | CO COBALT (II) ION × 2 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.40 Å |
| 1XNZ Crystal Structure of Mn(II) form of E. coli. Methionine Aminopeptidase in complex with 5-(2-chlorophenyl)furan-2-carboxylic acid 提交 2004-10-05 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–264（264 aa）
|
未记录 | MN MANGANESE (II) ION × 2 NA SODIUM ION × 1 FCD 5-(2-CHLOROPHENYL)FURAN-2-CARBOXYLIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.5；292 K；15% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, temperature 292K
|
分辨率 1.52 Å R-free 0.250 |
| 1YVM E. coli Methionine Aminopeptidase in complex with thiabendazole 提交 2005-02-16 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–264（264 aa）
|
突变：R175Q | CO COBALT (II) ION × 4 NA SODIUM ION × 1 TMG 2-(1,3-THIAZOL-4-YL)-1H-BENZIMIDAZOLE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.1；293 K；PEG 4000, cobalt chloride, hepes, methionine, sodium chloride, potassium chloride, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 1.60 Å R-free 0.233 |
| 2BB7 Mn Form Of E. coli Methionine Aminopeptidase In Complex With a quinolinyl sulfonamide inhibitor 提交 2005-10-17 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–264（264 aa）
|
未记录 | MN MANGANESE (II) ION × 3 NA SODIUM ION × 1 QMS N-(QUINOLIN-8-YL)METHANESULFONAMIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 1.70 Å R-free 0.233 |
| 2MAT E.COLI METHIONINE AMINOPEPTIDASE AT 1.9 ANGSTROM RESOLUTION 提交 1999-03-29 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–264（264 aa）
|
突变：R175Q | CO COBALT (II) ION × 3 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.1；CRYSTALS OF THE CO(II)-SUBSTITUTED ENZYME
WERE GROWN AT ROOM TEMPERATURE BY VAPOR
DIFFUSION IN 20-30 UL SITTING DROPS AFTER
MIXING THE PROTEIN, 12 MG/ML SOLUTION IN
STORAGE BUFFER(25 MM HEPES PH 6.8, 25 MM
K2SO4, 100 MM NACL, 1 MM COCL2, 15 MM
METHIONINE),CONTAINING 48.8 MM N-OCTANOYL
SUCROSE, 1:1 WITH WELL SOLUTIONS (24-26% PEG4000, 0.1M HEPES PH7.0-7.2,FRESH 2 MM COCL2)., pH 7.1, VAPOR DIFFUSION, HANGING DROP
|
分辨率 1.90 Å |
| 3MAT E.COLI METHIONINE AMINOPEPTIDASE TRANSITION-STATE INHIBITOR COMPLEX 提交 1999-03-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–264（264 aa）
|
突变：R175Q | CO COBALT (II) ION × 2 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.1；CRYSTALS OF THE CO(II)-SUBSTITUTED ENZYME
WERE GROWN AT ROOM TEMPERATURE BY VAPOR
DIFFUSION IN 20-30 UL SITTING DROPS AFTER
MIXING THE PROTEIN, 12 MG/ML SOLUTION IN
STORAGE BUFFER(25 MM HEPES PH 6.8, 25 MM
K2SO4, 100 MM NACL, 1 MM COCL2, 15 MM
METHIONINE),CONTAINING 48.8 MM N-OCTANOYL
SUCROSE, 1:1 WITH WELL SOLUTIONS (24-26%
PEG4000, 0.1M HEPES PH7.0-7.2,FRESH
2 MM COCL2). CRYSTALS WERE OBTAINED OF THE
INHIBITOR COMPLEX BY INCUBATING THE PROTEIN
AS ABOVE AT ROOM TEMPERATURE FOR 5 MIN WITH
A 20-FOLD MOLAR EXCESS OF THE INHIBITOR DISSOLVED
IN DMSO. THE FINAL INHIBITOR:ENZYME RATIO
WAS 10:1 (1% DMSO) AFTER MIXING THE PREFORMED
COMPLEX WITH WELL SOLUTION (0.1M MES PH 6.1,
|
分辨率 2.00 Å |
| 4MAT E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT 提交 1999-03-29 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–264（264 aa）
|
突变：R175Q, H79A | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；Crystals of the HIS79ALA mutant were obtained by mixing the apoenzyme
retaining the C-terminal HISs-tag (6.7 mg/ml, 20 mM DTT, 25 mM HEPES
pH 6.8, 25 mM K2SO4, 100 mM NaCl) with an equal volume of well solution
(22-27 % PEG 3400, 0.1 M HEPES pH 7.0, 200 mM NaCl). Diffraction quality
crystals were obtained after macroseeding into 20 uL hanging drops.
|
分辨率 2.00 Å |