Current Protein Identity:P09064
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3J81 CryoEM structure of a partial yeast 48S preinitiation complex Deposited 2014-08-29 | Assembly 1 Protein–RNA Heteromer;Protein × 39 PDB declaration: 42-meric(42) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 3 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT;pH 6.5;20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 seconds before plunging;120 K;Cryogen ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I)
|
Resolution 4.00 Å |
| 3JAP Structure of a partial yeast 48S preinitiation complex in closed conformation Deposited 2015-06-18 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 4 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT;pH 6.5;20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 to 3 seconds before plunging;120 K;Cryogen ETHANE;Blot for 2.5 to 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
|
Resolution 4.90 Å |
| 6FYX Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1) Deposited 2018-03-12 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 117 ZN ZINC ION × 5 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6FYY Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C2) Deposited 2018-03-12 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 117 ZN ZINC ION × 5 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 6GSM Structure of a partial yeast 48S preinitiation complex in open conformation. Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain l
127–270(144 aa)
|
Not recorded | 7NO [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{S})-2-azanyl-4-methylsulfanyl-butanoate × 1 MG MAGNESIUM ION × 82 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.15 Å |
| 6GSN Structure of a partial yeast 48S preinitiation complex in closed conformation Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain l
127–270(144 aa)
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.75 Å |
| 6I3M eIF2B:eIF2 complex, phosphorylated on eIF2 alpha serine 52. Deposited 2018-11-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 16 PDB declaration: hexadecameric(16) Consistent with protein count |
Chain M
1–285(285 aa)
Chain N
1–285(285 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions for sample preparation were made fresh, filter sterilized, and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE;Blot for 2 seconds before plunging
|
Resolution 3.93 Å |
| 6I7T eIF2B:eIF2 complex Deposited 2018-11-17 | Assembly 1 Protein heterocomplex Heteromer;Protein × 16 PDB declaration: hexadecameric(16) Consistent with protein count |
Chain M
1–285(285 aa)
Chain N
1–285(285 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;Solutions for sample preparation were made fresh, filter sterilized, and degassed.
cryo-EM vitrification conditions
Cryogen ETHANE;Blot for 1 second before plunging
|
Resolution 4.61 Å |
| 6QG0 Structure of eIF2B-eIF2 (phosphorylated at Ser51) complex (model 1) Deposited 2019-01-10 | Assembly 1 Protein heterocomplex Heteromer;Protein × 16 PDB declaration: hexadecameric(16) Consistent with protein count |
Chain O
1–285(285 aa)
Chain P
1–285(285 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.15 Å |
| 6QG1 Structure of eIF2B-eIF2 (phosphorylated at Ser51) complex (model 2) Deposited 2019-01-10 | Assembly 1 Protein heterocomplex Heteromer;Protein × 16 PDB declaration: hexadecameric(16) Consistent with protein count |
Chain O
1–285(285 aa)
Chain P
1–285(285 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.25 Å |
| 6QG2 Structure of eIF2B-eIF2 (phosphorylated at Ser51) complex (model A) Deposited 2019-01-10 | Assembly 1 Protein heterocomplex Heteromer;Protein × 16 PDB declaration: hexadecameric(16) Consistent with protein count |
Chain O
1–285(285 aa)
Chain P
1–285(285 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.55 Å |
| 6QG3 Structure of eIF2B-eIF2 (phosphorylated at Ser51) complex (model B) Deposited 2019-01-10 | Assembly 1 Protein heterocomplex Heteromer;Protein × 16 PDB declaration: hexadecameric(16) Consistent with protein count |
Chain O
1–285(285 aa)
Chain P
1–285(285 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.40 Å |
| 6QG5 Structure of eIF2B-eIF2 (phosphorylated at Ser51) complex (model C) Deposited 2019-01-10 | Assembly 1 Protein heterocomplex Heteromer;Protein × 16 PDB declaration: hexadecameric(16) Consistent with protein count |
Chain O
1–285(285 aa)
Chain P
1–285(285 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.10 Å |
| 6QG6 Structure of eIF2B-eIF2 (phosphorylated at Ser51) complex (model D) Deposited 2019-01-10 | Assembly 1 Protein heterocomplex Heteromer;Protein × 16 PDB declaration: hexadecameric(16) Consistent with protein count |
Chain O
1–285(285 aa)
Chain P
1–285(285 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.40 Å |
| 8RW1 Structure of a yeast 48S-AUC preinitiation complex in closed conformation Deposited 2024-02-02 | Assembly 1 Protein–RNA Heteromer;Protein × 38 PDB declaration: 41-meric(41) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 115 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.35 Å |
| 8S8D Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-2) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 38 PDB declaration: 41-meric(41) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 116 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.45 Å |
| 8S8E Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-3.1) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 40 PDB declaration: 43-meric(43) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 116 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.85 Å |
| 8S8F Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-3.2) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 39 PDB declaration: 42-meric(42) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 116 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.95 Å |
| 8S8G Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-2.1) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 38 PDB declaration: 41-meric(41) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 116 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8S8H Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-2.2) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 38 PDB declaration: 41-meric(41) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 116 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8S8I Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-eIF1) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 39 PDB declaration: 42-meric(42) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 117 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8S8J Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-eIF5) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 39 PDB declaration: 42-meric(42) Consistent with all polymers |
Chain l
1–285(285 aa)
|
Not recorded | MG MAGNESIUM ION × 115 ZN ZINC ION × 4 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 8S8K Structure of a yeast 48S-AUC preinitiation complex in swivelled conformation (model py48S-AUC-swiv-eIF1) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 40 PDB declaration: 43-meric(43) Consistent with all polymers |
Chain l
1–270(270 aa)
|
Not recorded | MG MAGNESIUM ION × 96 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 9F79 Crystal structure of the S. cerevisiae eIF2beta N-terminal tail bound to the C-terminal domain of eIF5 Deposited 2024-05-03 | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric(6) Consistent with protein count |
Chain C
39–106(68 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;283 K;0.4 M (NH4)2SO4, 0.08 M Li2SO4
|
Resolution 2.00 Å R-free 0.228 |